神经干细胞移植延缓失神经肌萎的作用机制

Objective To study the effect and mechanism of transplanting spinal fetal neural stem cells (NSCs) into the peripheral nerve for delaying muscle denervation atrophy. Methods Spinal. fetal NSCs were separated from spinal cord of enceinte 10 to 12 days SD rats, cultured and purified. After three passages, the formed NSC spheres were blew into single cell suspension ( 106/μl×5 μl) and transplanted into the distal part of the transected tibial nerve. 5 μl of cell culture medium was injected into the distal tibial nerve in the control group. Three and 5 months after the transplantation, the distal part of the tibial nerve and the triceps suraes were harvested and identified with specific markers, by means of indirect immunofluorescent staining to evaluate survival and differentiation of transplanted NSCs in the nerve, and to observe the neuromuscular junctions.Results Compare to the control group, atrophy of the triceps suraes muscle was less severe 3 and 5 months after NSCs transplantation. Postsynaptic membrane was also better preserved in NSCs transplanted group. Five months after NSCs transplantation, new synapses (neuromuscular junction) formed in the denervated muscle.Conclusion NSCs transplantation can delay atrophy denervated muscles. NSCs transplantation can not only maintain the structure of postsynaptic membrane, but also form new synapse with the denervated muscle.     

CHARACTERIZATION OF VPI AS IMMUNODOMINANTANTIGEN OF ENTEROVIRUS TYPE 70 ABID ANTIGENICANALYSIS OF VIRUS STRAINS BY MONOCLONAL ANTIBODJES

The neutralizing antigens of eliterovirus type70(E70) are characterized immunochemically. It wasfound that all of the 7 neutralizing McAbs testedcould cambine with both naturally-occurring topcomponents (NTC) and virions in immunodot test,five of them could combine with VPl in the irmnuno-hlot system, and patient sera and antibodies fromimmune and hyperimmune ammals couId only cam-bine with VPl. Furthermore. 10 McAbs (includingthe mentioned 7) were used to analyse the antigenic differences of 8 E70 strains isolated in China in 1985 and the prototype virus J670/71. The results indicate that the 10 McAbs can neutralize all 9 viruses almostequally. The data indicate that VPl is immuno-dominant ancl stable enough to keep ycutralizingtem.     

STUDIES ON PERIPHERAL T LYMPHOCYTE SUBSETS IN PATIENTS WITH AUTOIMMUNE THYROID DISEASES

Peripheral T lymphocyte subsets in 60 cases of Graves disease (GD) and 16 cases of Hashimoto’s thyroiditis (HT) were determined by the use of OKT monoclonal antibodies (McAb). The results showed that in both diseases OKT3 cells (total T cells ) and OKT8 cells（suppressor T cells, TS cells ） were lower and the ratio of LKT4 cells (helper T cells, TH cells )/ OKT8 cells was much higher than those in normal controls. GD patients who were treated with antithyroid drugs (ATD) and whose serum T3, T4 recovered to normal, could be further divided into subgroups of high OKT4 / OKT8 cell ratio and of normal ratio. The pathogenesis, treatment and relapse of autoimmune thyroid diseases (AITD) were discussed in this paper on the basis of the results.     

生脉饮对肺纤维化模型大鼠支气管肺泡灌洗液中谷胱甘肽含量的影响

1　材料和方法1 1　药物与试剂　平阳霉素为天津河北制药厂产品 ,批号 980 6 0 8;生脉饮口服液为北京同仁堂制药二厂产品 ,批号 82 6 0 2 6 8;还原型谷胱甘肽 ,Ｓｉｇｍａ公司出品 ,上海华美公司分装 ,批号 991110。1 2　动物分组及处理方法　实验动物为河南医科大学实验动物中心提供的Ｗｉｓｔａｒ雌性健康大鼠 ,体重 2 0 0ｇ左右 ,随机分为①正常对照组 ,②模型对照组 ,③用药组。正常对照组不做任何处理 ,模型组及用药组按文献方法[1 ] 气管内滴入平阳霉素 ,用量为 5ｍｇ/ｋｇ体重 ,造模后第 2ｄ开始用药 ,用药组按 2ｍｌ/ 10 0ｇ体重用…     

绝对期青光眼自发眼球破裂1例

患者男,７１岁。因右眼间歇性胀痛３天突发剧痛出血１天,于１９９９年１月６日１１∶００急诊入院。患者曾于１９９６年２月在我院诊断为右眼闭角型青光眼而采用常规抗青光眼药物治疗,症状好转出院。出院后视力渐降。１９９７年６月右眼视力完全丧失,因感右眼胀痛诊断为“右眼绝对期青光眼”再次住院治疗。常规抗青光眼药物治疗后,症状缓解,视力无改善出院。自行间断滴用抗青光眼眼药水。１９９９年１月５日晚睡觉时,突觉右眼剧烈疼痛,右眼球不断向外涌出大量鲜血（约５０ｍｌ）。于１９９９年１月６日诊断为“右眼角膜葡萄肿”收住…     

葛根素对胰岛素抵抗大鼠骨骼肌中GSK-3表达的影响

目的观察葛根素对高脂高糖饮食诱导的胰岛素抵抗模型大鼠骨骼肌中糖原合成酶激酶-3(GSK-3)表达的影响.方法将30只Wistar大鼠随机分为正常组、模型组和葛根素组,每组10只.模型组和葛根素组均喂以高糖高脂饲料,4周后葛根素组大鼠给予100mg/kg葛根素腹腔注射,连续6周.定期检测其体重、血甘油三酯和胆固醇、空腹血糖和胰岛素水平,并计算胰岛素敏感指数.实验结束后采用Western blot法检测各组大鼠骨骼肌中GSK-3的含量.结果模型组大鼠骨骼肌中GSK-3的含量较正常组增高70.20%(P＜0.01),给予葛根素治疗6周后,大鼠骨骼肌中GSK-3的含量较模型组减少26.54%(P＜0.01),较正常组增高25.02%(P＜0.05).结论葛根素可降低胰岛素抵抗大鼠骨骼肌细胞GSK-3蛋白表达水平,从而加强葡萄糖的摄取和利用,改善胰岛素抵抗.     

静脉注射丙种球蛋白无反应型川崎病合并重症感染1例报告

川崎病（KD）是一种原因不明的以全身中、小血管炎为主要病变的儿童急性发热性疾病。如未经治疗,约20％～25％的KD患儿可发生冠状动脉损害（CAL）。尽管目前标准化方案已广泛应用,但仍有10％～15％的患儿对静脉注射丙种球蛋白无反应,且这部分患儿发生CAL的风险升高。尤其合并重症感染更加重冠状动脉损害的风险,因此,早期识别及有效治疗KD十分重要。     

肱三头肌及指总伸肌电生理支配权重分析在同侧C7神经根移位术中的临床意义

目的 分析在健侧C7神经根移位术中得出的臂丛神经各干对肱三头肌/指总伸肌的电生理支配权重,推断其对于同侧C7神经根移位术的影响,为探讨其安全性和适应证提供电生理依据.方法 随机选择2007年8月-2007年10月15例全臂丛神经根性撕脱伤,行健侧C7神经根移位的患者.男13例,女2例:年龄18～49岁,平均28岁.致伤原因:坠落伤1例,压砸伤2例,余均为车祸伤.左侧8例,右侧7例.术中依次刺激健侧臂丛神经上、中、下干,于肱三头肌/指总伸肌记录复合肌肉动作电位(compound muscle action potential,CMAP),比较各干的CMAP波幅所占百分比,确定臂丛神经各干对肱三头肌,指总伸肌的电生理支配权重.术后6个月内随访肱三头肌/指总伸肌肌力,并复查肌电图了解其自发电活动和主动募集反应情况.结果 15例患者均获随访6个月.肱三头肌的电生理支配权重:上中干型3例(20%),中下干型3例(20%),全干型7例(47%),中干型2例(13%);指总伸肌:中下干型3例(20%),全干型10例(67%),下干型2例(13%).肱三头肌:术后1个月,2例出现肱三头肌肌力4级,募集反应单纯相,至术后3个月恢复正常.余患者术后1个月肌力均达5级,募集反应单纯混合相或混合相.指总伸肌:术后1个月,患者肌力及募集反应均恢复正常.结论 对于各种电生理支配权重的患者,C7神经根的切取均不会造成肱三头肌和指总伸肌的实质性损害,同侧C7神经根移位术是安全可行的;但对于中干支配权重高的患者,采用同侧C7神经根全干移位应慎重,有可能造成肱三头肌短期内的肌力下降.     

高频电针刺激“内关”穴和非穴后对心肌缺血大鼠“内关”“天泉”穴皮肤血流灌注量的影响

目的:研究高频电针刺激"内关"穴和非穴后对大鼠"内关""天泉"穴皮肤血流灌注量的影响。方法:选取30只雄性Wistar大鼠按照随机数字表的方法分成正常组、假手术组、模型组、高频电针"内关"组、高频电针非穴组5组。高频电针"内关"组采取每日电针大鼠左侧"内关"穴,高频电针非穴组采取每日电针非穴。于治疗结束30 min后,应用激光散斑衬比成像技术检测各组大鼠双侧"内关""天泉"穴区域的皮肤血流灌注量。结果:与正常组比较,模型组各穴位血流灌注量均降低(P0.05),且双侧"内关"穴区皮肤血流灌注量显著升高(P0.05)。结论:高频电针"内关"穴组心肌缺血情况有所改善,且心肌处于不同状态时,"内关"穴区皮肤血流灌注量变化显著。