云南高原平坝血吸虫病重流行区春秋季查螺效果比较

在云南高原血吸虫病流行区 ,查螺是消灭血吸虫病的关键措施之一 ,为了解什么季节查螺最好 ,查出的钉螺密度高 ,阳性钉螺多 ,查多大范围方能达到理想的效果 ,是一个值得探讨的问题 ,笔者在中和试区进行了探索 ,现将结果报告如下。1　试区概况中和行政村位于洱海南端的邓川盆地内 ,面积约3 km2 ,地势西北高东南低 ,为亚热带季风气候 ,年均气温1 3 .7℃ ,降雨量 71 7mm。全村 4个自然村 ,1 2个农业社 ,5 60户 ,2 2 78人 ,有黄牛 2 5 0头。该村是洱源县血吸虫病高度流行区 ,历史累计钉螺面积 46.43万 m2 ,病人 1 1 96人。自 1 993年以来实行人畜…     

麻醉深度监测及麻醉质量控制

麻醉工作主要任务之一是为手术病人提供无痛、安全、良好的手术条件。麻醉医师需从各种监测反颌信息中，分析、综合和判断病人的各项生命机能指标，并按需进行适时的调整和干预，以使各项生命体征尽可能保持在正常或接近正常的生理状态。由于病人存在着各种各样的个体差异性，麻醉药物种类日渐增多，     

孕妇传染病血清流行病学监测报告

孕妇传染病血清流行病学监测报告郝宗宇，张涛，吕家锐，张爱梅，付玉生，武林，姜艳玲，王华钦，孟淑霞，王广科，徐曼莉，郝应平，芦素莲，赵红英，赵妮出生缺陷是世界各国政府和人民都极为重视的一大社会问题，优生优育是提高国民素质的重要保证。但许多病原生物因素无...     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

硬膜外腔注射生理盐水对剖宫产术患者腰麻效果的影响

目的探讨硬膜外腔注射生理盐水对剖宫产术患者腰麻效果的影响。方法择期行子宫下段剖宫产术患者60例,年龄24～30岁,体重59～73 kg,随机分为2组,每组30例,A组蛛网膜下腔注射规定剂量的0.75%布比卡因后硬膜外腔注射生理盐水5 ml;B组蛛网膜下腔注射0.75%布比卡因。按序贯法进行试验,设定布比卡因的起始剂量为9 mg,剂量梯度为1.5 mg,若上一例有效,则下一例递减一个剂量梯度,若无效则下一例递增一个剂量梯度,蛛网膜下腔阻滞有效的标准为注射布比卡因后20 min内阻滞上平面达T5。采用概率单位法计算ED50。结果A组布比卡因的ED50（5.8 mg）低于B组（8.1 mg）,两组比值为0.72,95%置信区间为0.27～0.98,区间范围不包括1,差异有统计学意义（P〈0.05）。结论硬膜外腔注射生理盐水可增强剖宫产术患者腰麻的效果。     

胆系感染中毒性休克

胆系感染中毒性休克新泰市中医院（２７１２００）韩庆琏，张涛，王渊患者女，５５岁。１９７４年春以胆系感染中毒性休克急症入镇医院外科治疗，给予抗生素、升压药物、维生素、输液等对症治疗３天，病情无好转，外科无手术指征而请中医会诊。患者呈中度昏迷状态，全身黄...     

肾盂输尿管连接部梗阻手术治疗

肾盂输尿管连接部梗阻手术治疗燕东亮，周荣祥，杨存让，侯成玉，孟凡学，迟玉友，尹洪山（滨州市附属医院泌尿外科，滨州市２５６６０３无棣县医院外科）关键词尿路梗阻；肾盂成形术；手术治疗我院１９８８～１９９５年收治肾盂输尿管连接部梗阻（Ｐｅｌｖｉｃ－Ｕｒｅｔ...     

神经元性一氧化氮合酶活性在app/ps1双转基因AD模型小鼠皮质和海马的分布

本实验用 4～ 5月龄和 15～ 16月龄的 app/ ps1dtg小鼠各 5只 ,同龄野生型小鼠每个年龄组各 5只 ,用β-NADPH组织化学染色显示神经元一氧化氮合酶活性 ,刚果红组织学染色显示神经炎性斑 ,无偏性体视学计量皮质和海马神经炎斑的总体积 ,研究神经元一氧化氮合酶活性在 app/ ps1双转基因 (app/ ps1dtg) AD模型小鼠大脑皮质和海马的异常分布 ,探讨其在该模型小鼠及 AD患者脑内病理改变中的作用。结果显示 ,n NOS阳性神经元在各组小鼠皮质和海马内的分布没有区别 ,4～ 5月龄 app/ps1dtg小鼠皮质和海马可见神经炎斑 (NP)和营养不良性 NOS阳性神经突 (DTN) ;15～ 16月龄 app/ ps1dtg小鼠皮质和海马内NP的体积分别是 4～ 5月龄 app/ ps1dtg小鼠皮质 NP的 5倍 (P<0 .0 0 5 )和 8倍 (P<0 .0 0 1) ;15～ 16月龄 app/ ps1dtg小鼠皮质内 DTN的体积明显增大 (P<0 .0 1) ;且伴有 NOS阳性神经元形态的改变及海马 CA1 区 NOS阳性神经元数量的减少 (P<0 .0 5 ) ,DTN的形成与 NP呈正相关关系 (r=0 .85 ,P<0 .0 5 )。本实验结果表明 ,app/ ps1dtg小鼠能够模拟 AD患者脑内 NOS阳性神经元的病理改变 ,DTN的形成可能与 Aβ的毒性作用有关 ,DTN产生的 NO可能参与 app/ ps1dtg小鼠和 AD的神经病理过程。