农村防保所存在问题及对策探讨

通过实地走访、召开座谈会、下发调查表等形式,对金湖县乡镇防保所现况及存在问题进行了调查分析,并对深化农村防保体制改革作了初步探讨.     

盐酸恩丹西酮、地塞米松和胃复安对抗肿瘤药物所致呕吐的治疗作用

在肿瘤的化疗过程中,许多抗肿瘤药如：顺铂、氮芥、环磷酰胺、阿霉素、5-氟尿嘧啶、氨甲喋呤、丝裂霉素、长春新碱等在抑制生长或杀伤肿瘤细胞的同时，对机体内继续繁殖的正常细胞同样有毒害作用，其代谢产物直接刺激延髓呕吐感受区，同时胃肠道粘膜上皮细胞也遭受毒害．导致一系列胃肠道症状如食欲不振、恶心、呕吐等。作者对12例不同的癌症患者在化疗的同时加用盐酸恩丹西酮（On－dansetron）、地塞米松和胃复安控制呕吐在应．取得满意的效果，观介绍如下。1．治疗方法：在化疗药物治疗当天，在给药前40min予他塞米松10mg静脉注射．在给…     

Construction of Antisense Transforming Growth Factorβ_1 Gene and Its Effect on the Proliferation by Expression in Osteosarcoma Cells

Summary:To construct the antisense transforming growth factorβ1(TGFβ1)gene and investigatethe effect of TGFβ1 autocrine loop blockage on the proliferation of osteosarcoma cells.TGFβ1 cDNAwas cloned by RT-PCR from human osteosarcoma cells(MG-63)and inserted into pcDNA_3 to con-struct an antisense expression vector,which was dubbed pcDNA_3-TGFβ1(-).MTT was used to de-tect the proliferation of osteosarcoma cells transfected by antisense TGFβ1 gene.Our results showedthat the proliferation of the transfected osteosarcoma cells was suppressed markedly.It is concludedthat TGFβ1 autocrine loop blockage in osteosarcoma cells could inhibit cell proliferation,which mightbe helpful for gene therapy of osteosarcoma.     

The Effect of Aging on Erectile Function Induced by Apomorphine and Electric Field Stimulation to Rat

Population aging has become a world wide tendency and according to the UNproject,the proportion of population above60 years old would exceed the proportionof children below1 5years old by2 0 50 .Erectile dysfunction(ED) seriously affectsthe quality of lif…     

Effects of basic fibroblast growth factor on biological characteristics of osteoblasts

Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro. Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF ( 5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 ( TGF-β1 )was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Resu/ts: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β1 mRNA increased significantly, but the intracellular ALP content decreased. Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.     

癌基因c—erb B—2在结肠血吸虫病并发肠癌和肠息肉中的表达及AgNOR观察

探讨肠道血吸虫病与肠癌发生的关系。应用免疫组织化学ＬＳＡＢ法结肠血吸虫病并发结肠癌，肠息肉和无血吸虫病的肠部，肠息肉行ｃ－ｅｒｂＢ－２标记并ＡｇＮＯＲ染色。结果；肿瘤细胞中癌基因ｃ－ｅｒｂＢ－２表达呈明显异质性；血吸虫病并发肠癌与无血吸虫病的肠癌表达阳性率，两者之间无明显；血吸虫病肠息肉的表达明显高于无血吸虫病的肠息肉。     

带锁髓内钉固定并植骨治疗陈旧性股骨干骨折

目的 评价带锁髓内钉并植骨治疗股骨干陈旧性骨折的临床疗效和优点。方法 对1996年2月～2003年11月收治的47例陈旧性股骨干骨折患者进行回顾性分析。其中植骨组28例，非植骨组19例。采用切开复位，带锁髓内钉扩髓，静力型固定，根据骨折部位生长情况改为动力型固定。结果 术后随访39例，随访lO～25个月，平均随访时间14．2个月。植骨组骨折愈合率为95．6％(22／23)，感染率为4．3％(1／23)，骨折平均愈合时间为6．3个月，非植骨组则分别为62.5%(10／16)，6．25％(1／16)和9．7个月。临近关节功能达正常，无感染、断钉等并发症。结论 带锁髓内钉静力固定能有效控制有害的剪应力，同时对骨折有一定的加压作用，可提供早期坚强的内固定，同时，植骨后骨折愈合快，并发症少，是治疗陈旧性骨折的理想方法。     

经肝动脉与门静脉同时插管灌注化疗治疗晚期肝癌

<正> 1988年5月至2000年5月我们对不能手术切除的31例晚期肝癌患者经肝动脉与门静脉同时插管、间断灌注化疗,临床观察治疗效果尚满意,现总结报告如下。 1 临床资料 1.1 一般资料:31例中男27例,女4例,年龄最小37岁,最大68岁,平均年龄54例。原发性肝癌24例,弥漫性肝癌7例,均经病理诊断证实。 1.2 辅助治疗:为完成整个治疗过程,减少化疗药物抑制造血系统的毒性作用,保护消化功能。本组在化疗灌注的前、中、后同时应用以下辅助治疗:①聚肌胞2ml肌注或静滴2～3次/周。②干扰素1支,肌注1～3次/周。③华蟾素10～30mg     

跨供区髂骨皮瓣一期修复肢体大面积骨与软组织缺损

Objective To study the clinical effect of iliac flap straddled donor area to primary repair large defect of bone and soft tissue. Methods Through the anatomical study about iliac flap, the new oper-ation method about iliac flap was designed, which straddled donor area and used one blood vessel stem (deep iliae circumflex artery) and two branch blood vessel(deep iliac circumflex artery and superficial iliac circum-flex artery compounding in series) for blood supply. Blood supply area of the analogical operation was stud-ied by irrigating fresh cadaver with Indian ink. Thirty-two patients with large defect of bone and soft tissue (20 males, 12 females, 17-68 years old, average 36 years old) were repaired by this method, and the hemo-dynamics was studied by laser Doppler flowmetry. Results It was feasible to connect superficial iliac cir-cumflex artery with the branch of deep iliac circumflex artery. Indian ink irrigating fresh cadaver simulate operation showed that area scope dyed by Indian ink: in ilium, Indian ink was found in the bone which was (15.8±3.2) cm away from anterior superior iliac spine. In skin flap, Indian ink was found (8.6±2.4) cm superi-or, (5.4±2.6) cm inferior, (5.8±2.6) cm internal and (24.6±5.8) cm external from the anterior superior iliac spine. Laser Doppler flowmetry showed that the peripheral blood flow of skin flap increased as time passed, the increase speed began to tardy at the third day, then ingress the platform stage. Thirty-two cases were all succeed with functional rehabilitation and perfect shape. Thirty-one cases got completely bone healing; bone healing time ranged from 9 to 19 months, with an average of 12 months. One case turned to chronic os-teomyelitis, and had to suffer operation again, the bone healing time was 22 months. Conclusion As the ili-ae flap straddled donor area has sufficient blood supply, it can repair large defect of bone and soft tissue in one stage.     

足背动脉跨区供血的足背及足底内侧联合皮瓣的解剖研究与临床应用

目的探讨应用同一血管蒂（足背动脉）跨供区（足背与足底内侧）联合皮瓣游离移植的可行性。方法对足背和足底内侧2个皮瓣进行解剖学研究。采用10只成人新鲜足标本分别从足背动脉（5只）、足底内侧动脉（5只）加压灌注碳素墨汁甲醛。在足底和足背不同部位取全层皮肤做脱水透明标本处理，在显微镜下对0．3-0．5mm厚铺片进行观察，以确定不同动脉的供血范围。设计以足背动脉供血的跨足背与足底内侧2个皮瓣供区的新皮瓣移植术式，并用来修复手部大面积皮肤缺损。结果足背皮瓣与足底内侧皮瓣存在丰富的交通血供区，从足背动脉灌注碳素墨汁甲醛，其分布范围除整个足背外，在足底分布如下：以足底中线纵轴为基线可及中线外侧（1．7±0．5）cm；以足底中线横轴为基线，上可及中线上（4．5±1．5）cm，下可及中线下（3．8±0．9）cm。从足底内侧动脉灌注碳素墨汁甲醛，其分布范围除整个足底内侧区域外，在足背区可及躅长伸肌外侧（0．6±0．5）cm。以足背动脉供血的跨区联合皮瓣血供丰富，临床应用12例全部成功。结论以足背动脉供血的跨区联合皮瓣切取面积大，血供可靠，可以用来修复手部大面积皮肤缺损。