枝江县沮漳河滩钉螺及感染性钉螺分布的研究

采用系统抽样法调查湖沼型洲垸亚型流行区的沮漳河西岸枝江段面河滩钉螺及感染性钉螺的分布状况,结果河滩有螺面积214万m~2,共调查9247框,活螺框出现率14.40％,活螺密度0.68只/0.11m~2,感染螺框出现率2.54％,感染螺密度0.042只/0.11m~2,钉螺感染率6.13％。不同环境、不同段面螺情各异。96.89％的钉螺和96.13％的感染性钉螺集中分布在距河堤150m以内的洲滩。从而明确了区域性灭螺的范围。     

肝硬化患者PiM亚型等位基因频率的分布及其临床意义

肝硬化患者ＰｉＭ亚型等位基因频率的分布及其临床意义中国医科大学第一临床学院消化内科（１１０００１）姜敏，姜若兰，刘金兰，黄玉红中国医科大学法医学系血清教研室铁坚本实验就血清中具有遗传多态性的ＰＩＭ进行聚丙烯酰胺凝胶等电聚焦法的谱型分析。Ｐｉ（α１－抗...     

血浆交换中控制研究的评价观察

这项研究试图解释文献中报道的血浆交换(PE)中控制试验的不佳结果和未控制试验的较好结果。这项工作是通过观察血浆交换的各种不同治疗变化,即:治疗的频率和长度、血浆交换的量和类型、设置PE的早与晚和蛋白重组现象的压制来完成的。在慢性进行性多发硬化、风湿性关节炎和急性血管阻     

喉创伤的处理与护理

喉创伤不但战时发病率高,在近年亦有迅速增加的趋势。严重者常因呼吸道梗阻、大血管损伤出血,并发重症感染而死亡。如后遗喉瘢痕狭窄,不仅影响功能,且处理也非常棘手。为此,本病除及时正确的诊治外,整个病程严密地观察和细心地护理亦至为关键。现将我院1962年10月至1990年10月收治的38例喉创伤护理体会介绍如下。临床资料患者共38例,男31例,女7例。年龄最小的2岁,最大的86岁,平均28岁。闭合性喉损伤24例,除喉外软组织挫伤,15例有喉内会厌处粘膜充血气肿、瘀血,9例有皮下气肿,2例有环构关节脱位。就诊时14例有不同程度的呼吸费力症状。开放性损伤14例。其中切割伤12例,弹片贯通伤2     

联合化疗引起肝肾功能和心肌损害51例临床分析

５１例恶性肿瘤和白血病化疗后出现肝功能损害２０例，肾功能损害２例，心肌损害３１例，受损病例总数为３６例。     

Selection and their antitumor activity of antisense oligonucleotides targeting messenger RNA of vascular endothelial growth factor receptor 2

Objective： To select the antisense oligonucleotides （asONs） which hybridize with the mRNA of vascular endothelial growth factor receptor2 （VEGFR2, also named as kinase insert domain-containing receptor：KDR） in an effective and specific way, and to investigate their antitumor activity in MCF-7 cells. Methods： The effective antisense oligonucleotides were chosen by computer prediction combined with oligonucleotide microarrays. The inhibition effect on MCF-7 cells proliferation was measured by MTT; and VEGFR2 expression was surveyed by Western-blotting and RT- PCR. Results： Using predicting secondary structure of VEGFR2 mRNA with RNA folding program, computer prediction designed 30 antisense oligonucleotide probes that were directed to local loose regions of RNA structure. In 30 probes, 4（4/30, 13.33%） antisense oligonucleotides showed strong hybridization intensities in oligonucleotide microarrays test and were selected. All these antisense oligonucleotides targeting 4 different sites of VEGFR2 mRNA lowered the level of VEGFR2 mRNA and protein present in MCF-7 cells. Proliferation of MCF-7 cells was reduced by 4 antisense oligonucleotides, respectively, in which asON1 was the most effective, with the inhibitory rates being 53.06% at 0.8 I.tmol/L. Conclusion： Combination of computer prediction with oligonucleotide microarrays is an effective way in selecting optimal antisense oligonucleotides. The antisense oligonucleotides showed good correlation between their antitumor activity and the hybridization intensities. The antisense oligonucleotides targeting VEGFR2 mRNA demonstrated prominent antitumor role in vitro.     

国产要素饮食治愈消化道瘘4例报告

国产要素饮食系我院与上海东海制药厂协作以酶法水解鱼蛋白制成,它是一种平衡、全面、配比适当的营养物,含有18种必需和非必需氨基酸及部分短肽,40%脂肪乳剂由大豆油与大鲨鱼肝油乳化而成,并加添葡萄糖、多种维生素、无机盐等。于1979年6月～     

多媒体技术在脑电图继续教育中的应用

在脑电图继续教育中借助多媒体技术 ,利用其图像、声音、动画能完美结合的优势 ;利用其"海纳百川"的独特库容量 ,不仅能克服传统教学方法中抽象、呆板等诸多不足 ,还能将临床上最新发现直观、生动、深入浅出地传授出去 ,明显提高了教学效果。