非穿透性小梁手术与小梁切除术治疗青光眼的远期疗效对照研究

Objective To evaluate clinical outcomes of non-penetrating trabecular surgery (NPTS)and trabeculectomy surgery (TS) in the treatment of primary open angle glaucoma (POAG). Methods It was a case-control study.A total of 63 patients (63 eyes) with POAG were observed retrospectively.Thirty one eyes and 32 eyes underwent NPTS and TS,respectively.Intraocular pressure(IOP),filtration bleb,visual field and post-operative complications were observed for 6-60 months.The CMH X2 test was used to analyse the difference of them.Results After operation.the IOP in the NPTS group were from(13.87±4.88)mm Hg(1 mm Hg=0.133 kPa) to (24.01±6.55)mm Hg,the IOP in the TS group were from(11.90±4.92)mm Hg to(19.10±7.43)mm Hg.The IOP in the NPTS group was significantly higher than that in the TS group (F=5.137,P<0.05).The ratio of sustained filtration bleb of NPTS group after surgery was 25/31(80.6%),while 6/31 were flat filtration bleb.There were statistically significant difference in the rate of disappearance of filtration bleb between these two groups(X2=8.129,P<0.05).The difference of visual field loss postoperatively between these two groups was not statistically significant.The incidence rate of newly developed cataract after NPTS and TS was 6/31 and 12/32.respectively.The difierence of rate of complication between these two groups was statistically non-significant(X2=3.797,P<0.05).The successful rate after NPTS and TS was 61.54%(16/26)and 14.29%(4/28),respectively.The difference of successful rate between these two groups was statistically significant(X2=14.463,P<0.05).Conclusions Both NPTS and TS are effective methods for the treatment of POAG.Postoperative complications after NPTS are less than those of TS.But patients with TS could maintain a lower IOP than those with NPTS.Long-term efficacy of NPTS is uncertain.it's important to choose the suitable surgery to gain a high success rate.     

人眼虹膜色素上皮细胞体外培养、冻存与复苏

目的：建立人眼虹膜色素上皮细胞体外培养并对其进行冻存与复苏。方法：直接刮取虹膜色素上皮细胞组织碎屑进行培养,光镜观察生长特性及形态特点,透射电镜观察其超微结构。根据慢冻速融的细胞冻存原则,定期收集细胞进行液氮冻存,至少2个月后进行复苏。结果：人眼虹膜色素上皮细胞体外培养成功,原代细胞在光镜下呈多角形单层生长,胞浆内有丰富的色素颗粒;电镜下见胞浆富含色素、细胞器丰富、细胞膜有明显微绒毛、微丝、相监细胞之间可见桥粒连接。共冻存6批细胞,进行4次复苏实验均成功,每镒复苏细胞存活为90％。结论：人眼虹膜色素上皮细胞体外培养的建立及冻存、复苏的成功,为研究某些疾病提供有利的基础。