Proteinase activity in the isolates of Trichomonas vaginalis according to their pathogenicity

Ten axenic isolates of Trichomonas vaginalis were subcutaneously injected to the BALB/c mice in order to assess their pathogenicity by means of so-called "mouse assay" method. All the isolates revealed neutral and acid proteinase activities both in their lysates and in culture media, but the specific activities of both proteinases in the severely pathogenic group were significantly higher than the mildly pathogenic group (p < 0.05). In the SDS-PAGE system in which the electrophoretic gels contained 0.4% gelatin as the substrate, five different banding patterns of trichomonal proteinases were detected, and the patterns were closely related with the pathogenicity of the isolates of T. vaginalis. All five bands might be regarded as cysteine proteinases group in the inhibitor assays. The cytotoxicity of the lysates of T. vaginalis to the target Chinese hamster ovarian (CHO) cell line was also significantly different according to the pathogenicity of the isolates, and generally lower in the lysates treated with cysteine proteinase inhibitors than in the control lysates. In summarizing the results, it might be considered that the proteinases of T. vaginalis showing five electrophoretic banding patterns are closely related with the pathogenicity and cytotoxicity of the isolates of T. vaginalis.     

Sequence-based differentiation of strains in the Mycobacterium avium complex

The complete 16S-23S rDNA internal transcribed spacer (ITS) was sequenced in 35 reference strains of the Mycobacterium avium complex. Twelve distinct ITS sequences were obtained, each of which defined a "sequevar"; a sequevar consists of the strain or strains which have a particular sequence. ITS sequences were identified which corresponded to M. avium (16 strains, four ITS sequevars) and Mycobacterium intracellulare (12 strains, one ITS sequevars). The other seven M. avium complex strains had ITS sequences which varied greatly from those of M. avium and M. intracellulare and from each other. The 16S-23S rDNA ITS was much more variable than 16S rDNA, which is widely used for genus and species identification. Phylogenetic trees based on the ITS were compatible with those based on 16S rDNA but were more detailed and had longer branches. The results of ITS sequencing were consistent with the results of hybridization with M. avium and M. intracellulare probes (Gen-Probe) for 30 of 31 strains tested. Serologic testing correlated poorly with ITS sequencing. Strains with the same sequence were different serovars, and those of the same serovar had different sequences. Sequencing of the 16S-23S rDNA ITS should be useful for species and strain differentiation for a wide variety of bacteria and should be applicable to studies of epidemiology, diagnosis, virulence, and taxonomy.     

Definition of the Linear Region of X-Ray -Induced Cable Response

Cable response to X-rays is linear with incident fluence, provided the deposited charge in cable dielectrics is directly proportional to the X-ray flux. To estimate the level at which the linear region ends, we discuss three nonlinear processes that modify the deposited charge profile in a hypothetical cable model: field-limiting in vacuum gaps, ionization effects in air-filled gaps, and radiation-induced dielectric conductivity. The exact level at which limiting of the Norton driver in an elemental length of cable begins depends on the cable geometry and the X-ray source. Estimates of the onset of nonlinearities caused by field-limiting and by dielectric conductivity are found in terms of cable and source parameters. With air-filled gaps, the Norton driver is always nonlinear. In addition to limiting of the Norton drivers, the load response of a long cable may be limited because propagating currents are attenuated by the induced conductivity of the bulk of the dielectric.     

A-IMilano apoprotein. Decreased high density lipoprotein cholesterol levels with significant lipoprotein modifications and without clinical atherosclerosis in an Italian family

Significant hypertriglyceridemia with a very marked decrease of high density lipoproteins (HDL)-cholesterol levels (7-14 mg/dl) was detected in three members (father, son, and daughter) of an Italian family. The three affected individuals did not show any clinical signs of atherosclerosis, nor was the atherosclerotic disease significantly present in the family. Lipoprotein lipase and lecithin:cholesterol acyltransferase activites were normal or slightly reduced. Morphological and compositional studies of HDL in the subjects showed a significant enlargement of the lipoprotein particles (approximately 120 vs. approximately 94 A for control HDL) and a concomitant increase in the triglyceride content. Analytical isoelectric focusing of HDL apoproteins provided evidence for multiple isoproteins in the apoprotein(apo)-A-I range, with nine different bands being detected instead of the usual four bands observed in normal subjects. Two-dimensional immunoelectrophoresis against apo-A antiserum indicated a clear reduction of apo-A in the alpha electrophoretic region, with splitting of the protein "peak." The observation in otherwise clinically healthy subjects of hypertriglyceridemia, reduced HDL-cholesterol, and marked apoprotein abnormalities, without a significant incidence of atherosclerotic disease in the family suggests this is a new disease entity in the field of lipoprotein pathology, very probably related to an altered amino acid composition of the apo-A-I protein (see Weisgraber et al. 1980. J. Clin. Invest. 66: 901-907).     

Strategic piggybacking--a self-subsidization strategy for nonprofit institutions

Nonprofit institutions often find that their socially worthwhile primary missions generate deficits. The author proposes a strategy for funding these shortfalls that is a synthesis of the specialization and diversified portfolio strategies. Following the method of strategic piggybacking, an organization should acquire or develop a business that is new for the institution and that may be unrelated to the institution's primary mission. The purpose of this new activity is to subsidize, at least in part, the deficit-producing primary mission.     

Grafting of enzymes on collagen films using Woodward''s reagent "K" and a water-soluble carbodiimide derivative

Two new methods of activation were developed to graft enzymes on collegen films. They involved chemical modifications of surface groups of collagen either by Woodward's reagent "K" or by EDC, a water-soluble derivative of carbodiimide. EDC was a better coupling agent and a detailed study was conducted with this agent. It could be used either in a global method of activation and coupling, or in a two-step procedure of activation of collagen, followed by spontaneous coupling of enzyme. All enzymes tested were successfully bound: malate dehydrogenase, lactate dehydrogenase, aspartate aminotransferase, urease, creatine kinase, hexokinase. The influence on the yield of grafted enzyme, of pretreatment of films, time and temperature of EDC activation, concentration of EDC and enzyme, protecting agents was studied. Stability of enzyme activity on storage was greatly increased after grafting. A co-grafted dual system creatine kinase/heoxkinase, was achieved which exhibited a good efficiency. A striking renaturing process at 0-4degreesC after thermal denaturation, was observed with hexokinase.     

In-network performance of handheld mobile terminals

This paper considers a realistic evaluation of the power mobile handsets are able to transmit and receive. It has been suggested to use the so-called total radiated power (TRP) and the total isotropic sensitivity (TIS) for the uplink and downlink, respectively, which may be seen as special cases of the general mean-effective-gain (MEG) measure. These measures are computed from the spherical radiation pattern of the handset and the different measures are obtained by using different models of the mobile propagation environment. In this paper, the results obtained via the spherical radiation patterns are compared with the equivalent performance obtained in a live Global System for Mobile Communications (GSM) network using data from the Abis network interface. This method does not require altering of the handsets and the testing uses normal calls in the network. The investigation is based on measurements with four different commercially available handsets carried out in two different indoor environments and involving 22 test users. In addition, a series of measurements were also made with a phantom simulating the handset user, allowing a test of how well the phantom represents the average user.     

On-Line Edge-Coloring with a Fixed Number of Colors

We investigate a variant of on-line edge-coloring in which there is a fixed number of colors available and the aim is to color as many edges as possible. We prove upper and lower bounds on the performance of different classes of algorithms for the problem. Moreover, we determine the performance of two specific algorithms, First-Fit and Next-Fit . Specifically, algorithms that never reject edges that they are able to color are called fair algorithms. We consider the four combinations of fair/ not fair and deterministic/ randomized. We show that the competitive ratio of deterministic fair algorithms can vary only between approximately 0.4641 and 1/2, and that Next-Fit is worst possible among fair algorithms. Moreover, we show that no algorithm is better than 4/7-competitive. If the graphs are all k -colorable, any fair algorithm is at least 1/2-competitive. Again, this performance is matched by Next-Fit while the competitive ratio for First-Fit is shown to be k/(2k-1) , which is significantly better, as long as k is not too large.     

Optimization of practical trusses with constraints on eigenfrequencies, displacements, stresses, and buckling

In this paper we consider the optimization of general 3D truss structures. The design variables are the cross-sections of the truss bars together with the joint coordinates, and are considered to be continuous variables. Using these design variables we simultaneously carry out size optimization (areas) and shape optimization (joint positions). Topology optimization (removal and introduction of bars) is only considered in the sense that bars of minimum cross-sectional area will have a negligible influence on the performance of the structure. The structures are subjected to multiple load cases and the objective of the optimizations is minimum mass with constraints on (possibly multiple) eigenfrequencies, displacements, and stresses. For the case of stress constraints, we deal differently with tensile and compressive stresses, for which we control buckling on the element level. The stress constraints are imposed in correlation with industrial standards, to make the optimized designs valuable from a practical point of view. The optimization problem is solved using SLP (Sequential Linear Programming).