Linkage map of Escherichia coli K-12, edition 10: the physical map

A physical map, EcoMap10, of the now completely sequenced Escherichia coli chromosome is presented. Calculated genomic positions for the eight restriction enzymes BamHI, HindIII, EcoRI, EcoRV, BglI, KpnI, PstI, and PvuII are depicted. Both sequenced and unsequenced Kohara/Isono miniset clones are aligned to this calculated restriction map. DNA sequence searches identify the precise locations of insertion sequence elements and repetitive extragenic palindrome clusters. EcoGene10, a revised set of genes and functionally uncharacterized open reading frames (ORFs), is also depicted on EcoMap10. The complete set of unnamed ORFs in EcoGene10 are assigned provisional names beginning with the letter "y" by using a systematic nomenclature.     

Serum from diabetic BB/W rats enhances calcium currents in primary sensory neurons

We examined the hypothesis that exposure of nondiabetic rat dorsal root ganglion (DRG) neurons to sera from diabetic BB/W rats would produce an increase in calcium currents associated with impaired regulation of the inhibitory G protein-calcium channel complex. Acutely dissociated rat DRGs were incubated for 18-24 h in medium supplemented with sera (10% vol/vol) from either diabetic rats with neuropathy or age-matched, nondiabetic controls. Exposure of DRG neurons to sera from diabetic BB/W rats resulted in a surface membrane immunofluorescence pattern when treated with an anti-rat light-chain antibody that was not observed in neurons exposed to control sera. Calcium current density (IDCa) was assessed with the use of the whole cell variation of the patch-clamp technique. IDCa in neurons exposed to diabetic sera was significantly increased compared with neurons exposed to control sera. Guanine nucleotide-binding (G) protein regulation of calcium channel function was examined with the use of a two-pulse "facilitation" or IDCa enhancement protocol in the presence of activators [guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S)] or antagonists [guanosine 5'-O-(2-thiodiphosphate) (GDP beta S) and pertussis toxin (PTX)] of G protein function. Facilitation was significantly decreased in neurons exposed to diabetic sera. Intracellular diffusion of neurons with GDP beta s blocked facilitation, whereas dialysis with GTP gamma s increased facilitation to a similar magnitude in neurons exposed to either diabetic or control sera. Treatment with PTX resulted in a significant increase in IDCa and approximately 50% decrease in facilitation in neurons treated with control sera but no significant changes in neurons exposed to diabetic sera. We conclude that serum from diabetic BB/W rats with neuropathy contains an autoimmune immunoglobulin that impairs regulation of the inhibitory G protein-calcium channel complex, resulting in enhanced calcium influx. Regulation of the inhibitory G protein-calcium channel complex involves PTX-sensitive and -insensitive G proteins.     

Ecstasy--cool dope with long-lasting effects?

The amphetamine derivative MDMA (3,4-methylenedioxymethamphetamine) was first synthesised in 1914 as an appetite suppressant, but was never used as such. MDMA is commonly known as "ecstasy" and has become a popular recreational drug of abuse at dance-clubs and rave parties, where it is combined with all-night dancing, crowded conditions, poor hydration and loud sound. This combination is probably the main reason why we have seen an upsurge in toxicity problems at rave parties, since all these factors are thought to promote or increase the toxicity of MDMA. The desired effects of MDMA are euphoria, increased energy and enhanced communication with others. Adverse effects are hyperthermia, rhabdomyolysis, acute renal failure, hepatotoxicity, depression and psychosis.     

Profibus-DP在连铸辊道控制系统上的应用

介绍攀钢1#方坯连铸后区辊道控制的基本原理,概述西门子S7-400PLC与M440变频器通过现场总线Profibus-DP怎样连接起来,阐述PLC硬件组态如何进行、变频器相应参数如何设置,并提出PLC和变频器之间进行数据交换的方法。     

USB移动存储设备的惟一性识别方法研究

人员和设备的认证识别是信息系统安全首要解决的问题,根据涉密存储介质保密管理的要求,论述了移动存储设备惟一性标识的必要性.为了更加安全可靠地做到移动存储设备的惟一性识别,研究了移动存储设备的惟一标识管理技术,以U盘为例概括了几种不同的USB移动存储设备的标识方法.根据惟一性标识码的选择和存放要求,对这几种方法的存在问题及优缺点进行分析比较,提出了一种基于特殊命令请求的惟一性识别方法.     

多小波基多尺度多传感器数据融合

多传感器数据融合技术已经被广泛应用在多个领域,小波多尺度分解对数据的分析具有独特的优点,小波基的选择对数据融合结果也起着关键的作用.提出一种新的基于多个小波基的数据融合算法,先对含有噪声的传感器信号进行多个不同小波基的多尺度分解,然后对相同小波基分解的信号在多尺度上实施加权数据融合算法,之后进行不同小波基的逆变换得到的重构信号,最后将基于不同小波基的重构信号做最终的融合.实验结果表明:数据融合技术可以从多个方面多个层面以及多种融合原则来考虑,从而融合众多的因素得到最优的结果.     

基于无线通信的设备点检系统

针对传统设备点检系统工作效率低、劳动强度大、准确性低、实时性差等问题,提出了一种基于无线通信的设备点检系统。该系统结合了单片机、无线通信和计算机信息管理等技术,具有多元化通信方式,包括无线射频(RF)通信、GSM通信和Internet通信,能够在线检测现场设备的振动、温度、压力等主要参数,并能根据环境情况进行预警,同时具有远程监测功能。测试结果表明,该系统稳定性强,显著提高了设备点检系统的操作管理品质。     

中国传统符号的演变——门

人类学会构筑房屋并为自己的进出留下一个进出方便的口,门就产生了。门是一个极为抽象的形态,一个变化万千的符号,这个古老的符号——门的形态变化一直在随着科技的发展而演变,无论是古人还是后人都在其中精心地演绎着中国的传统民族文化。     

具有多维特征的WSN路由协议研究

为实现不同地理维数数据类型的合理融合,提出一种基于通用搜索树(GiST)的分簇拓扑结构。利用叶节点的位置信息建立簇,通过GiST体系结构算法对区域内的所有簇进行分块组织,建立树形拓扑结构。仿真结果表明,GiST体系结构算法具有良好的多维特性,可快速进行不同维数结构间的融合。建立的结构与其他拓扑结构相比,网络传输效率较高,网络生命周期较长。