Light atom derivatives of structure-preserving sugars are unconventional negative stains

Although glucose and certain other sugars are known to greatly reduce distortion and denaturation of proteins during drying, use of this monosaccharide as an experimental negative stain does not permit imaging of lattice periodicities in test specimens of thin catalase crystals. However, the potassium and sodium salts of several forms of monophosphorylated glucose (200 mM), diphosphorylated glucose, monosulfated glucose, maltose-1-phosphate, and trehalose-6-phosphate, all dry into a glassy layer and scatter transmitted electrons sufficiently to show the 86 A major periods in catalase crystals. Glucose-6-phosphate provides sufficient image contrast at concentrations from 2 mM (=0.067%) to 500 mM (= 16.8%). Underfocusing increases visualization of the periodic lattice, indicating a large contribution of phase contrast to these images. Upon exposure to the electron beam, thicker regions of derivatized saccharides or pure glucose develop bubbling; this redistribution of dried stain largely can be precluded by imaging with low-dose exposures. Power spectra of images of catalase crystals contained within 200 mM disodium glucose-6-phosphate show that periodic information can be recorded to 21 A; some individual features of dipotassium glucose-6-phosphate distribution within the protein lattice have a measured width of around 5 A. The experimental results demonstrate that structure-preserving mono- and di-saccharides also serve successfully as negative stains after they are coupled to light atom scatterers.     

Design of low-cost 183 GHz subharmonic mixers for commercial applications

Techniques are presented for designing fixed-tuned millimetre-wave components with the least number of parts to minimise the cost and maximise the potential for volume manufacture. Design techniques for millimeter-wave mixer components targeted at potentially high volume applications, such as medical and security screening and nondestructive testing, require certain compromises in the use of CAD tools. The compromise techniques are demonstrated using commercially available foundry diodes in the design of a 183 GHz subharmonic mixer for earth observation applications. The resulting mixer exhibits 6.85 dB double sideband conversion loss and a mixer temperature of 988 K using 5 mW of local oscillator power at 92 GHz     

Broad-area InAs/GaAs quantum dot lasers incorporating intermixed passive waveguide

InAs/GaAs quantum dot lasers incorporating passive waveguide created by post-growth intermixing processing have been studied. Emission wavelength of the passive section shows relative blueshift as high as 135 nm with respect to the emission wavelength of the active section. Intrinsic losses in the section formed by the intermixing are very small. Broad-area lasers with 100 mum stripe width incorporating intermixed section have demonstrated improvements in far-field pattern under both pulsed and continuous wave pumping current     

Effects of phentermine on responding maintained under multiple fixed-ratio schedules of food and cocaine presentation in the rhesus monkey

Drugs that decrease drug-maintained responding at doses that do not decrease other behaviors in animals may be suitable candidates for development as medications to treat drug abuse in humans. The present study examined whether this effect could be obtained with phentermine, a drug that has been reported to decrease cocaine intake in humans. Rhesus monkeys were trained under multiple fixed-ratio 30-response schedules of food and i.v. cocaine delivery. Phentermine was always given as a slow, i.v. infusion. Acute treatment with phentermine (0.3-10 mg/kg) decreased cocaine-maintained responding at doses that did not decrease, or decreased less, food-maintained responding for each of three unit doses of cocaine (10-100 microg/kg/injection). Subacute treatment with phentermine (3 or 5.6 mg/kg, daily) also decreased cocaine-maintained responding more than food-maintained responding. After subacute treatment was terminated, rates of cocaine-maintained responding generally recovered to levels comparable to those seen during untreated control sessions. Phentermine (0.3-3 mg/kg) did not generally increase responding associated with a very low (1 microg/kg/injection) unit dose of cocaine, suggesting that the decrease in cocaine-maintained responding at higher unit doses was not the result of a leftward shift in the cocaine unit dose-effect function. Phentermine (0.1-3 mg/kg) decreased responding maintained by 1-[2-[bis(4-fluorophenyl) methoxy]ethyl]-4-[3-phenylpropyl] piperazine (GBR 12909) (30 microg/kg/injection) at doses similar to those that decreased food-maintained responding. These results show that phentermine is effective in decreasing cocaine self-administration and suggest that it may be an effective medication for cocaine abuse.     

Future Directions for Data Compilations

As the world's supply of thermophysical property data that emerges from the laboratory increases, users of data become more dependent on evaluated compilations. However, the cost of producing and maintaining such compilations by traditional methods is becoming prohibitively expensive. The traditional compiler searches the literature, extracts, evaluates, and analyzes pertinent data and gathers it into a document or electronic database that reflects the state of knowledge of a particular subject at a particular time. Because clef the inherent time lag, it never catches up to the current state. The attempt to catch up requires that the whole procedure be repeated at intervals, with greater cost for each cycle. A more cost-effective procedure, called dynamic compilation is described. Here the user produces a compilation to-order at the time of need. It uses a suitable archive of experimental data maintained up-to-date, an automated procedure for extracting and selecting the best pertinent data, and procedures for fitting the pieces to suitable models that furnishes parameters for internally consistent data sets. With proper design of components this procedure is more economical than and superior to the traditional static compilations.     

Use of poly(tetrafluoroethylene)s as a sample support for the MALDI-TOF analysis of DNA and proteins

A proline rich antigen (PRA), which protects mice against Coccidioides immitis, has been analyzed for differential expression and variation among isolates. Northern blots of RNA from different stages of growth were probed with previously cloned cDNA and showed that mRNA for PRA increased as spherules transformed and matured from mycelia. This pattern corresponds to the relative potency of whole cell vaccines from similar preparations. The PRA gene was then cloned from a genomic library of the Silveira strain of C. immitis and its sequence analyzed. Eight other coccidioidal isolates, selected for diversity in geographic origin and resulting clinical disease, demonstrated heterogeneity in Southern blots and in sequences of polymerase chain reaction products. Silveira differed from other California isolates at 33 of 555 bases, whereas it differed from non-California isolates by <=2 bases. Only one of these substitutions affected protein sequence. Thus, tests or vaccines based on this gene are likely to cover most isolates.