Differences in the liability to self-administer intravenous cocaine between C57BL/6 x SJL and BALB/cByJ mice

Application of animal models of psychostimulant abuse for experimentation in mice is becoming increasingly important for studying the contribution of genetic differences, as well as the roles of selected (targeted) genes, in specific behaviors. The purpose of this study was to investigate strain differences in cocaine self-administration behavior between C57BL/6 x SJL hybrid mice and BALB/cByJ mice. These two strains were chosen because BALB/cByJ mice have a well-developed behavioral pharmacological profile, and hybrid strains on a C57BL/6 background are commonly used for generating transgenic expressing and knockout mutant mice. C57BL/6 x SJL mice dose-dependently acquired cocaine self-administration (1.0 mg/kg/injection but not 0.25 mg/kg/injection) by responding selectively in the active nose-poke hole and maintaining stable levels of daily drug intake; they also exhibited a characteristic inverted-U-shaped cocaine dose-effect function. BALB/cByJ mice failed to acquire cocaine self-administration at either dose under the same test conditions. The strain differences observed in self-administration did not seem to be attributed to other behavioral differences because the two strains exhibited similar amounts of spontaneous nose-poking in the absence of reinforcers, and BALB/cByJ mice responded more than C57BL/6 x SJL mice in a food-reinforced nose-poke operant task. Importantly, the dose-effect function for the motor stimulating effects of cocaine (3.8-30 mg/kg intraperitoneally) suggests enhanced sensitivity but reduced efficacy of cocaine in stimulating motor activity in BALB/cByJ mice relative to the C57BL/6 x SJL hybrid mice. These results indicate that the decreased liability of BALB/cByJ mice to acquire cocaine self-administration is not the result of differences in spontaneous activity or performance, but may reflect different sensitivities to the reinforcing, or rate-disrupting, properties of cocaine. The data support an influence of genetic background in the liability to self-administer cocaine. Thus, a hypothesis is proposed that the decreased liability of BALB/cByJ mice to acquire cocaine self-administration is related to differences in brain monoamine systems linked to the high "emotionality" profile of BALB/c mice in novel or fearful situations, including perhaps cocaine administration.     

Serendipity in diagnostic imaging: magnetic resonance imaging of the breast

BACKGROUND: Magnetic resonance imaging (MRI) of the breast has been proposed as a noninvasive diagnostic test for evaluation of suspicious ("index") lesions noted on mammography and/or clinical breast examination (CBE). However, women may have incidental ("serendipitous") lesions detected by MRI that are not found on mammography or CBE. To understand better whether or not biopsy procedures should be performed to evaluate serendipitous lesions, we estimated the breast cancer risk for women with this type of lesion. METHODS: A decision analysis model was used to estimate the positive predictive value (i.e., the chance that a woman with a serendipitous lesion has cancer) of MRI for serendipitous lesions in women who had an abnormal mammogram and/or CBE suspicious for cancer (where a biopsy procedure is recommended). We restricted the analysis to data from women whose index lesions were noncancerous and used meta-analysis of published medical literature to determine the likelihood ratios (measures of how test results change the probability of having cancer) for MRI and the combination of CBE and mammography. The positive predictive value of MRI was calculated using the U.S. population prevalence of cancer (derived from registry data) and the likelihood ratios of the diagnostic tests. RESULTS: Under a wide variety of assumptions, the positive predictive value of MRI was extremely low for serendipitous lesions. For instance, assuming sensitivity and specificity values for MRI of 95.6% and 68.6%, respectively, approximately four of 1000 55- to 59-year-old women with serendipitous lesions would be expected to have cancer (positive predictive value = 0.44%, 95% confidence interval = 0.24%-0.67%). CONCLUSION: In women with a suspicious lesion discovered by mammography and/or CBE that is found to be benign, serendipitous breast lesions detected by MRI are extremely unlikely to represent invasive breast cancer. Immediate biopsy of such serendipitous lesions may, therefore, not be required.     

Effects of mulch and mineral fertilizer on crop, weevil and soil quality parameters in highland banana

The decline of cooking banana production in parts of East Africa hasbeen associated with a loss of soil fertility and increased pest pressure.Previous work indicated that the use of mineral fertilizers at recommendedratesis not financially viable on plantations where pest pressure is high. Theobjectives of this study were to determine the effects of half the recommendedrates of mineral fertilizers and organic mulch on banana yield, soil and foliarnutrient status, soil water and pest damage in the central region of Uganda.Thestudy was conducted in a four-year old banana plantation infested with weevils.Treatments consisted of mineral fertilizer alone, mulch alone, a combination ofmulch with fertilizer, and a control (no mulch from outside the plot, ormineralfertilizer applied). Fruit yield was generally low (mean of 7.4 Mgha^–1 yr^–1) and increases above thecontrol ranged from 1.1 to 2.0 Mg ha^–1 yr^–1. Banana yield was significantly higher in the solemulch treatment than in the control. However, there were no significantdifferences in yield among the treatments that received external inputs. Thus,there was no demonstrable yield advantage of combining mineral fertilizer withmulch. At the end of the trial, soil K concentrations in the two treatmentsthatwere mulched, were almost twice those of the control, but this difference wasnotstatistically significant. Concentrations of Ca and Mg did not differ withtreatment. Soil available P significantly increased when mineral fertilizer andorganic mulch were combined. Treatments that received organic mulch were lowerin foliar concentrations of N and Mg, and higher in K. Soil water content wasgreater in the surface soil layer in the treatments that received mulch,presumably due to less surface run-off and evaporation, since there was nochangein surface soil porosity. The effect of organic and inorganic amendments had noimpact on weevil damage, which remained above 5% at the end of the trial. Weconclude that where weevil damage is over 5% of the cross section near thecollar, banana production may not be economically increased through use ofeither mineral fertilizer and/or organic mulch.     

Cardiac pacing--from then to now

Progress in both the health sciences and engineering sciences has been necessary for the development of the cardiac pacemaker. From the invention of the vacuum tube triode amplifier in 1906 to that of the oscillator just a few years later; from the early electrocardiograph in 1906 and the first stimulation of a dog's heart in 1927 to the application of electricity to the heart of a stillborn infant only 2 years later, engineering and medicine have progressed together to solve problems in pacemakers. The semiconductor transistor emerged in 1948, and in 1950 work was published on the open heart resuscitation of dogs with voltage pulses to the heart, work whose principle was applied soon after in a human patient with complete heart block. The development of the modern pacemaker has run an exciting course, including such phenomena as one engineer helping to design his own pacemaker. In the past 20 years, science has change the package, the power source, pacing mode, electrodes, and leads of pacemakers; it has improved their reliability and longevity, their programming, telemetry, and instrumentation.     

氰化工艺过程中金属存量计算方法探讨

为搞好金属平衡，减少理论与实际差值，依据某黄金矿山氰化工艺流程特点，对脱药用单层浓密切、漫出槽、三层洗涤浓密机三个作业中的金属存量的计算方法进行了探讨。     

Single amino acid substitutions can further increase the stability of a thermophilic L-lactate dehydrogenase

Lactate dehydrogenases are of considerable interest as stereospecificcatalysts in the chemical preparation of enantiomerically pure-hydroxyacid synthons. For such applications in synthetic organicchemistry it would be desirable to have enzymes which tolerateelevated temperatures for prolonged reaction times, to increaseproductivity and to extend then applicability to poor substrates.Here, two examples are reported of significant thermostabilizations,induced by sitedirected mutagenesis, of an already thermostableprotein, the L-lactate dehydrogenase (EC 1.1.1.27 [EC] , 35 kDa permonomer subunit) from Bacillus stearothermophilus. Thermal inactivationof this enzyme is accompanied by irreversible unfolding of thenative protein structure. The replacement of Argl71 by Tyr stabilizesthe enzyme against thermal inactivation and unfolding. Thisstabilizing effect appears to be based on improved interactionsbetween the subunits in the core of the active dimeric or tetramericforms of the enzyme. The thermal stability of L-lactate dehydrogenasevariants with an active site Arg residue, either in the 171(wild-type) or in the 102 position, is further increased bysulfate ions. The two stabilizing effects are additive, as foundfor the Argl71Tyr/ Gln1O2Arg double mutant, for which the stabilityof the protein in 100 mM sulfate solution reaches that of L-lactatedehydrogenases from extreme thermophiles. All mutant proteinsretain significant catalytic activity, both in the presenceand absence of stnhilfoing salts, and are viable catalysts inpreparative scale reactions.     

The Potential of Asiatic Acid in the Reversion of Cyclophosphamide-Induced Hemorrhagic Cystitis in Rats

The purpose of this study was to determine if asiatic acid may act efficiently in the model of cyclophosphamide (CYP)-induced cystitis in rats. We performed experiments after administration of CYP (single dose 200 mg/kg, intraperitoneally), asiatic acid (30 mg/kg/day for 14 consecutive days, by oral gavage), or CYP plus asiatic acid, during which conscious cystometry, measurements of urothelium thickness and bladder edema, as well as selected biomarkers analyses were conducted. In rats that received asiatic acid together with CYP, a drop in bladder basal pressure, detrusor overactivity index, non-voiding contraction amplitude, non-voiding contraction frequency, and the area under the pressure curve were observed, when compared to the CYP group. Furthermore, a significant increase in threshold pressure, voided volume, intercontraction interval, bladder compliance, and volume threshold to elicit NVC were found in that group accordingly. Administration of the asiatic acid successfully restored concentrations of biomarkers both in bladder urothelium (BDNF, CGRP, OCT-3, IL-1β, IL-6, NGF, nitrotyrosine, malondialdehyde, TNF-α, SV2A, SNAP23, SNAP25, PAC-1, ORM1, occludin, IGFBP-3, HB-EGF, T–H protein, Z01, and HPX) and detrusor muscle (Rho kinase and VAChT) in CYP-treated rats. Finally, asiatic acid significantly decreased urothelium thickness and bladder oedema. Asiatic acid proved to be a potent and effective drug in the rat model of CYP-induced cystitis.     

Review of Generalized anxiety disorders across the lifespan: An integrative approach.

Reviews the book Generalized anxiety disorder across the lifespan: An integrative approach (see record 2009-04840-000) by Michael E. Portman. Generalized Anxiety Disorder (GAD) perhaps might be construed to be the overlooked and neglected stepchild of the various anxiety disorders within the current psychopathological and psychotherapeutic literatures. Portman’s book is an important and largely successful attempt to fill this gap in the literature. In it, the author describes the clinical presentation and phenomenology of the disorder, considers the history of the anxiety disorders that were precursors to the current diagnostic category, reviews the process of, and criteria for, accurate diagnosis of GAD, and concisely summarizes the most important theoretical explanations of the etiology, structure, and reviews the most important and frequently cited treatments for GAD. This book would be a useful and important addition to the library of any graduate student or established researcher who is considering conducting research on any facet of GAD. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The Journal of Psychotherapy Integration at 20: An introduction to the special section.

Discusses the contents of this special issue. With this issue, the Journal of Psychotherapy Integration closes its 20th volume and therefore its 20th year of publication. The editor provides a history of some of the most memorable moments of the journal. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

开放式系统管理

开放式系统除具备已有的优势外,也构成了重大的信息技术挑战。终端用户只能过多地依靠自动化供应商协助他们保持过程控制网络的安全性。事实上,关于Et趋复杂的开放式自动化构架和专业化信息技术支持的工作正在由厂房工程师来担当。