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Drinking water biofilm formation on polyvinyl chloride (PVC), cross-linked polyethylene (PEX), high density polyethylene (HDPE) and polypropylene (PP) was followed in three different reactors operating under stagnant or continuous flow regimes. After one week, a quasi-steady state was achieved where biofilm total cell numbers per unit surface area were not affected by fluctuations in the concentration of suspended cells. Metabolically active cells in biofilms were around 17-35% of the total cells and 6-18% were able to form colony units in R(2)A medium. Microbiological analysis showed that the adhesion material and reactor design did not affect significantly the biofilm growth. However, operating under continuous flow (0.8-1.9 Pa) or stagnant water had a significant effect on biofilm formation: in stagnant waters, biofilm grew to a less extent. By applying mass balances and an asymptotic biofilm formation model to data from biofilms grown on PVC and HDPE surfaces under turbulent flow, specific growth rates of bacteria in the biofilm were found to be similar for both materials (around 0.15 day(-1)) and much lower than the specific growth rates of suspended bacteria (around 1.8 day(-1)). 相似文献
94.
Dr. Erik Strandberg Dr. Parvesh Wadhwani Dr. Jochen Bürck Dr. Patrick Anders Dr. Christian Mink Dr. Jonas van den Berg Raffaele A. M. Ciriello Dr. Manuel N. Melo Prof. Dr. Miguel A. R. B. Castanho Prof. Dr. Eduard Bardají Prof. Dr. Jakob P. Ulmschneider Prof. Dr. Anne S. Ulrich 《Chembiochem : a European journal of chemical biology》2023,24(4):e202200602
BP100 is a cationic undecamer peptide with antimicrobial and cell-penetrating activities. The orientation of this amphiphilic α-helix in lipid bilayers was examined under numerous conditions using solid-state 19F, 15N and 2H NMR. At high temperatures in saturated phosphatidylcholine lipids, BP100 lies flat on the membrane surface, as expected. Upon lowering the temperature towards the lipid phase transition, the helix is found to flip into an upright transmembrane orientation. In thin bilayers, this inserted state was stable at low peptide concentration, but thicker membranes required higher peptide concentrations. In the presence of lysolipids, the inserted state prevailed even at high temperature. Molecular dynamics simulations suggest that BP100 monomer insertion can be stabilized by snorkeling lysine side chains. These results demonstrate that even a very short helix like BP100 can span (and thereby penetrate through) a cellular membrane under suitable conditions. 相似文献
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FE Alexander 《Canadian Metallurgical Quarterly》1999,32(1):63-64
In India, cholera is endemic and affects usually the 3 to 5-year-old age group. There have been occasional reports in the neonatal period with Vibrio cholerae O139 Bengal. We report here a case of Vibrio cholerae O1 diarrhea in a 2-day-old, breastfed male, who had been delivered in the hospital and developed severe dehydration. 相似文献
97.
Borges C.L.T. Falcao D.M. Mello J.C.O. Melo A.C.G. 《Power Systems, IEEE Transactions on》2001,16(2):203-209
This paper describes two parallel methodologies for composite reliability evaluation using sequential Monte Carlo simulation. The methodologies are based on coarse grain asynchronous implementations. In the first methodology, a complete simulation year is analyzed on a single processor and the many simulated years necessary for convergence are analyzed in parallel. In the second methodology, the adequacy analysis of the system operating states within the simulated years is performed in parallel and the convergence is checked on one processor at the end of each simulated year. The methodologies are implemented on a 10 nodes IBM RS/6000 SP scalable distributed memory parallel computer and on a network of 8 IBM RS/6000 43P workstations. The results obtained in tests with actual power system models showed high speedup and efficiency on both parallel platforms 相似文献
98.
Different past experiences are likely to influence current preferences and consumption through learning processes, such as evaluative conditioning, leading to different flavour preference developments. The ability to measure past experience of alcoholic beverage consumption would be useful in order to understand the drivers of current wine consumption. The developed and validated tool provides reliable data for both past alcohol consumption recall and current wine consumption. Intraclass correlation coefficients (ICC) and F-tests indicated reliability, specifically, high internal consistency (lifetime drinking history and current wine consumption), internal validity and acceptable limits of agreement (current wine consumption relative to a 7-day diary), especially for the most frequently consumed wine (red), demonstrating that the measures taken were reproducible over time. In contrast to previous lifetime alcohol consumption studies, the present study included measures of type of wine consumed. Results justify and encourage the administration of the developed tool on a larger sample, aiming to seek associations between ‘drinking histories’ in order to understand current wine consumption. 相似文献
99.
Surgical correction of unroofed coronary sinus, left superior vena cava, dextrocardia, and situs solitus in a 4-month-old infant consisted of reroofing the coronary sinus by means of a left atrial flap while redirecting the left superior vena cava to the right atrium. Excellent access to the left side of the left atrium was afforded by the associated dextrocardia. 相似文献
100.
The effectiveness of insulin replacement therapy in the prevention of salt-induced hypertension in diabetes mellitus was examined using Alloxan diabetic rats. Early daily (eight units/day) treatment with insulin prevented the development of high blood pressure after six weeks of high-salt feeding. The mean arterial pressure (MAP) for the early insulin-treated and salt-fed group (DET-SF) was 123.37 +/- 6.37 mmHg which was close to the value for normal (control) rats 128.17 +/- 4.84 mmHg, but significantly (p < 0.001) less than that of the untreated diabetic salt-fed group (DSF) which was 164.58 +/- 8.33 mmHg. The nondiabetic salt-fed (NDSF) group had MAP of 150.27 +/- 4.24 mmHg. Late commencement of insulin therapy did not significantly affect the sensitivity of the diabetic rats to high-salt diet. The results suggest that early commencement of insulin therapy could prevent the development of high blood pressure in diabetic rats. 相似文献