Comparative characterization and cytotoxicity study of TAT-peptide as potential vectors for siRNA and Dicer-substrate siRNA

Recently, a newly discovered Dicer-substrate siRNA (DsiRNA) demonstrates higher potency in gene silencing than siRNA but both suffer from rapid degradation, poor cellular uptake and chemical instability. Therefore, Tat-peptide was exploited to protect and facilitate their delivery into cells. In this study, Tat-peptide was complexed with siRNA or DsiRNA through simple complexation. The physicochemical properties (particle size, surface charge and morphology) of the complexes formed were then characterized. The ability of Tat-peptide to carry and protect siRNA or DsiRNA was determined by UV-Vis spectrophotometry and serum protection assay, respectively. Cytotoxicity effect of these complexes was assessed in V79 cell line. siRNA-Tat complexes had particle size ranged from 186?±?17.8 to 375?±?8.3?nm with surface charge ranged from ?9.3?±?1.0 to +13.5?±?1.0?mV, depending on the Tat-to-siRNA concentration ratio. As for DsiRNA-Tat complexes, the particle size was smaller than the ones complexed with siRNA, ranging from 176?±?8.6 to 458?±?14.7?nm. Their surface charge was in the range of +27.1?±?3.6 to +38.1?±?0.9?mV. Both oligonucleotide (ON) species bound strongly to Tat-peptide, forming stable complexes with loading efficiency of more than 86%. These complexes were relatively non cytotoxic as the cell viability of ～90% was achieved. In conclusion, Tat-peptide has a great potential as siRNA and DsiRNA vector due to the formation of stable complexes with desirable physical characteristics, low toxicity and able to carry high amount of siRNA or DsiRNA.     

Optimization of biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent using response surface methodology

Clostridium butyricum EB6 successfully produced hydrogen gas from palm oil mill effluent (POME). In this study, central composite design and response surface methodology were applied to determine the optimum conditions for hydrogen production (P_c) and maximum hydrogen production rate (R_max) from POME. Experimental results showed that the pH, temperature and chemical oxygen demand (COD) of POME affected both the hydrogen production and production rate, both individually and interactively. The optimum conditions for hydrogen production (P_c) were pH 5.69, 36 °C, and 92 g COD/l; with an estimated P_c value of 306 ml H₂/g carbohydrate. The optimum conditions for maximum hydrogen production rate (R_max) were pH 6.52, 41 °C and 60 g COD/l; with an estimated R_max value of 914 ml H₂/h. An overlay study was performed to obtain an overall model optimization. The optimized conditions for the overall model were pH 6.05, 36 °C and 94 g COD/l. The hydrogen content in the biogas produced ranged from 60% to 75%.     

New Synthetic Control Charts for Monitoring Process Mean and Process Dispersion

A statistical quality control chart is widely recognized as a potentially powerful tool that is frequently used in many manufacturing and service industries to monitor the quality of the product or manufacturing processes. In this paper, we propose new synthetic control charts for monitoring the process mean and the process dispersion. The proposed synthetic charts are based on ranked set sampling (RSS), median RSS (MRSS), and ordered RSS (ORSS) schemes, named synthetic‐RSS, synthetic‐MRSS, and synthetic‐ORSS charts, respectively. Average run lengths are used to evaluate the performances of the control charts. It is found that the synthetic‐RSS and synthetic‐MRSS mean charts perform uniformly better than the Shewhart mean chart based on simple random sampling (Shewhart‐SRS), synthetic‐SRS, double sampling‐SRS, Shewhart‐RSS, and Shewhart‐MRSS mean charts. The proposed synthetic charts generally outperform the exponentially weighted moving average (EWMA) chart based on SRS in the detection of large mean shifts. We also compare the performance of the synthetic‐ORSS dispersion chart with the existing powerful dispersion charts. It turns out that the synthetic‐ORSS chart also performs uniformly better than the Shewhart‐R, Shewhart‐S, synthetic‐R, synthetic‐S, synthetic‐D, cumulative sum (CUSUM) ln S², CUSUM‐R, CUSUM‐S, EWMA‐ln S², and change point CUSUM charts for detecting increases in the process dispersion. A similar trend is observed when the proposed synthetic charts are constructed under imperfect RSS schemes. Illustrative examples are used to demonstrate the implementation of the proposed synthetic charts. Copyright © 2014 John Wiley & Sons, Ltd.     

Survey on communication architectures for wind energy integration with the smart grid

Communication systems are needed to integrate generated power from wind farms with the electrical grid. This paper provides a comprehensive review of available communication technologies, protocols and objectives related to wind energy and electrical grid integration. This paper summarizes the communication system solutions for wind generation. A major obstacle is an absence of unified communication architectures and standards.     

Exploring the internationalization of Malaysian contractors: the international entrepreneurship dimension

The internationalization of construction companies is a subject of enduring interest among construction management scholars. The internationalization pattern of Malaysian contractors was explored, this time mainly using the international entrepreneurship body of literature as the theoretical basis. In doing so, the focus of the internationalization process was on the entrepreneurs or top management. Data were collected with a postal questionnaire survey and follow‐up semi‐structured interviews. The findings underscore the importance of the entrepreneurs’ decision in the surveyed firms’ internationalization trajectory. Top management’s perception of how their companies might benefit from internationalization was the highest ranked foreign market entry consideration. Top managements’ initial and subsequent motivation also fashioned their companies’ degree of internationalization and tactics for securing contracts. The characteristics of the entrepreneurs which drove their companies to internationalize were also captured. The international entrepreneurship domain continues to expand into previously uncharted areas. As such there is potential to uncover even more interesting findings from future research endeavours on international contractors using the international entrepreneurship approach.     

Investigation of the impact of global warming on precipitation pattern of Saudi Arabia

This study has been carried out to forecast the impact of global warming on the precipitation pattern of Saudi Arabia by the end of year 2100. Simulation has been done using EdGCM model software (with available 8×10 resolution) developed at Columbia University on which there have been produced global precipitation maps for the seasonal and annual averages for the last 5 years (2096–2100). For each map, EdGCM grid values surrounding Saudi Arabia are used as input to one of the tools of Eagle point software called surface modelling (SM). SM is a new approach for downscaling global climate model results. SM software modelled out isohyets at 0.2 mm/day interval. The results indicate that the present pattern of precipitation (more in winter and less in summer) is going to change by almost equal occurrence of precipitation in all seasons for double_CO₂ (2CO₂) experiment. The 2CO₂ experiment indicates an increase of about 16.05% over the annual average precipitation across the country.     

The effect of combined use of chitosan and PIPS on push-out bond strength of root canal filling materials

Adhesion of root canal filling materials to root dentin is important for the long-term success of the treatment. Push-out bond strength test is used to evaluate the adhesion capacity of root canal filling materials to root canal walls. The aim of the present study is to compare the bond strength of root canal filling materials to root dentin after irrigation with EDTA, chitosan and the combination of chitosan and PIPS irridation using push-out bond strength test. Forty-eight extracted teeth were resected until 13-mm long roots were obtained. Root canals were prepared with a size-25 OneShape instrument. Samples were divided into three groups each including 15 roots. Group 1: Canals were rinsed with 0.2% chitosan and subjected to laser irridation with PIPS at the same time. Group 2: Canals were rinsed with 0.2% chitosan. Group 3: Canals were rinsed with EDTA. All canals were filled with .06 tapered gutta-percha and AH-plus sealer. One-mm thick slices were taken from coronal, middle and apical one-thirds of the roots. Push-out bond strength was determined using a Universal Testing Machine. One root from each group was observed under SEM to evaluate the degree of smear removal. Statistical analysis was performed with Kruskall-Wallis test. Results showed that bond strength values were statistically similar in overall evaluation for all groups (p > .05). In segmental evaluation, group 1 revealed the highest bond strength in apical one-third compared to other groups (p < .05).     

Investigation on the effect of spinning conditions on the properties of hollow fiber membrane for hemodialysis application

Polyethersulfone (PES) hollow fiber membranes were fabricated via the dry‐wet phase inversion spinning technique, aiming to produce an asymmetric, micro porous ultrafiltration hollow‐fiber specifically for hemodialysis membrane. The objective of this study is to investigate the effect of spinning conditions on the morphological and permeation properties of the fabricated membrane. Among the parameters that were studied in this work are air gap distance, dope extrusion rate, bore fluid flow rate, and the take‐up speed. The contact angle was measured to determine the hydrophilicity of the fibers. Membrane with sufficient hydrophilicity properties is desired for hemodialysis application to avoid fouling and increase its biocompatibility. The influences of the hollow fiber's morphology (i.e., diameter and wall thickness) on the performance of the membranes were evaluated by pure water flux and BSA rejection. The experimental results showed that the dope extrusion rate to bore fluid flow rate ratio should be maintained at 1:1 ratio to produce a perfectly rounded asymmetric hollow fiber membrane. Moreover, the flux of the hollow fiber spun at higher air gap distance had better flux than the one spun at lower air gap distance. Furthermore, spinning asymmetric hollow fiber membranes at high air gap distance helps to produce a thin and porous skin layer, leading to a better flux but a relatively low percentage of rejection for BSA separation. Findings from this study would serve as primary data which will be a useful guide for fabricating a high performance hemodialysis hollow fiber membrane. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43633.