一氧化碳中毒迟发性脑病38例临床观察

急性一氧化碳（CO）中毒迟发性脑病（delayed encephalopathy after acute carbon monoxide poisoning,DEACMP）是指急性一氧化碳中毒经抢救病情已好转或恢复,历时数日或数周表现正常或接近正常的“假愈期”,以后再次出现以神经精神症状为主的广泛性脑功能障碍,属临床常见病。现总结我院2004年12月至2007年4月收治的38例DEACMP患者,对其临床特点、发病机制及治疗进行分析如下。     

I-2190A Is a Potent Immunosuppressive Drug for Vascularized Heart Transplantation in Rats

I-2190AisamacrocyclictrieneantibioticthatwasdiscoveredinfermentationbrothoftheStreptomyceshygrocopicus.Intermsofphysicochemicalproperties,itisconfirmedthatI--2190AisthesameasSirolimus(Rapamycin),whichhasbeenprovedtohavepotentimmunosuppressiveactivity['.'].AsbothI--2190Aandsirolimusaremacrolidesandstructurallyhomologous,I--2190Awasinvestigatedtodetermineifithasimmunosuppressiveeffectlikesirolimus.Thepresentexperimentexaminedtheimmunosuppressiveeffectofi2190Aonvascularizedheartallograftsinra…     

非创伤性脑内血肿术后脑水肿影响因素分析

脑内血肿术后脑水肿是神经外科影响预后的重要因素 ,而脑水肿受多方面因素的影响。本文的目的在于总结脑水肿的影响因素。我科自 1996年至 1999年收治非创伤性脑内血肿病人 4 0例 ,现报告如下。1　临床资料与方法1·1　一般资料　男 2 8例 ,女 12例 ,年龄 4 0～ 70岁 ,平均年龄 (5 5 3± 6 75 )岁。入院时ＧＣＳ评分 :≤ 7分 16例 ,8～ 12分 2 4例。高血压性脑出血 32例 ,原因不明5例 ,多发动脉瘤合并动静脉畸形 1例 ,动静脉畸形 1例 ,硬脑膜动静瘘 1例。主要症状 :头痛 4 0例 ,呕吐31例 ,偏瘫 32例 ,抽搐 5例 ,躁动不安 12例 ,眩晕 2例…     

LPS通过p38MAPK-CBP诱导巨噬细胞RAW264.7表达和释放HMGB1

 目的 检测LPS刺激后小鼠腹腔巨噬细胞HMGB1和相关信号分子p38MAPK、NF-κB、CBP的表达,探讨脓毒症时巨噬细胞表达和释放HMGB1的信号传导机制。 方法 采用LPS刺激RAW264.7细胞,在不同的时间点用免疫细胞化学、激光共聚焦显微镜观察细胞内相关信号分子p38MAPK、NF-κB、CBP的变化,ELISA检测培养上清HMGB1的含量,Real-time PCR检测培养细胞HMGB1的mRNA水平,Western blot检测胞浆和胞核内HMGB1的含量。 结果 随着LPS的刺激,细胞浆内p38MAPK的绿色荧光逐渐增强,NF-κB的绿色荧逐渐减弱,而细胞核内NF-κB绿色荧光逐渐增强,CBP的绿色荧光逐渐增强,三者均于刺激后6 h达高峰。LPS刺激后12-48 h培养细胞胞浆和上清中HMGB1蛋白含量逐渐增加,而12-24 h胞核内HMGB1含量逐渐减少,36 h后又逐渐增多,各不同时间点差异具有显著性(P<0.01);而细胞内HMGB1 mRNA表达在LPS刺激后0-12 h无明显变化, 24 h、36 h和48 h明显增高,与0h相比差异有显著性(P<0.01)。 结论 LPS通过依次激活巨噬细胞内信号分子p38MAPK、NF-κB及CBP来诱导HMGB1的合成、转位和释放表达的。     

浅谈抗感冒药的应用及不良反应

抗感冒药多为非处方药,大病去医院,小病去药店,已被越来越多的患者所认同,如今感冒药成为许多家庭的必备药,而且往往不止一种.但由于相当数量的人缺乏相应医药知识,一旦患感冒后急于求成,常常几种感冒药同时服用,造成了如今抗感冒药在很大程度上的滥用.殊不知最常用的药品也可能造成严重的不良反应.若两种以上感冒药同时服用等于加大药物剂量.     

Lipo-PGE1治疗家兔肠系膜静脉血栓模型的实验研究

目的 探讨脂质微球化前列腺素E1(Lipo-PGE1)治疗家兔肠系膜静脉血栓(MVT)的可行性.方法 30只家兔随机平均分为3组,A、B两组建立MVT模型.A组(模型治疗组)采取帕肝素+Lipo-PGE1治疗,B组(模型对照组)单用帕肝素治疗,C组(非模型空白对照组)给予帕肝素治疗.分别于5个时间点,建模手术前(T1)...     

肝再生增强因子,细胞色素P450与人肝癌细胞在体外耐药的关系

目的:研究肝癌顺铂耐药细胞HepG2/cDDP中人肝再生增强因子(Human augmenter of liver regeneration,hALR)对细胞色素P450(Cytochrome P450,CYP450)表达的影响,观察ALR是否通过作用CYP450与肝癌细胞耐药的形成有关.方法:建立体外肝癌顺铂耐药细胞...     

凋亡抑制蛋白survivin在活化T细胞中的表达及其意义

目的: 探讨survivin在活化T细胞的表达及其意义。方法: 经丝裂原与同种抗原刺激激活T细胞, 免疫细胞化学染色后观察survivin和增殖细胞核抗原(PCNA)的表达。用流式细胞术检测CD3、CD25、Survivin的表达及细胞凋亡。给BALB/c裸鼠输注C57BL/6小鼠脾细胞后第 4~7天, 观察肝脏内T细胞浸润和survivin的表达。结果: ConA刺激后培养的脾细胞可表达survivin(表达不局限于G2 /M期 )。survivin 细胞为T细胞, T淋巴母细胞可同时表达CD25和survivin。survivin 细胞中仅部分细胞表达CD25。ConA刺激淋巴细胞后第 6天, 凋亡细胞的百分率明显增加 ( 22. 90% vs5. 23%P<0. 001)。于BALB/c裸鼠输注C57BL/6小鼠的脾细胞后,于第 4~7天, 在BALB/c裸鼠肝脏中可观察到T细胞浸润并表达Survivin。结论: T细胞激活后可表达survivin, 故可作为活化T细胞的标志。T细胞持续表达survivin, 需要在体内与抗原持续接触。     

结缔组织生长因子诱导人肾小管上皮细胞向间充质细胞转化的实验研究

Objective To investigate the role of connective tissue growth factor ( CTGF) in epithelial mesenchymal transition of HK-2 cells in vitro.Methods HK-2 cells were randomly divided into two groups; (1) control group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum only; and (2) experimental group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum and recombinant CTGF at a final concentration of 5 p-g/L The cells were collected at 72 h time points.Direct immunoiluorescence staining and immunohistochemistry were used to evaluate the E-cadherin,Vimentin,α-SMA and ERK2 in cells.Western-blotting was used to detect the E-cadherin,Vimentin and ERK2 protein expression.Boyden Chamber was used to detect the migration of tubular endothelium at 1 d,3 d and S d.Results There were less E-cadherin but more Vimentin expressed in cells of the experimental group.The presence of α-SMA was detected at 48 h with peak at 72 h in the cells of the experimental group.On the first day,the cellular migration in the two groups showed no difference.However,after 3 days,the transformed cells migrated surpassed the control group with peak at the 5th day [ (45.0±1.1) : (14.0±1.2),P < 0.05 ) ].Conclusion Connective tissue growth factor induces mesenchymal transformation of HK-2 cells,in which the ERK2 signaling pathway may play an important role.     

结缔组织生长因子诱导人肾小管上皮细胞向间充质细胞转化的实验研究

Objective To investigate the role of connective tissue growth factor ( CTGF) in epithelial mesenchymal transition of HK-2 cells in vitro.Methods HK-2 cells were randomly divided into two groups; (1) control group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum only; and (2) experimental group including cells cultured in DMEM medium supplemented with 10% fetal bovine serum and recombinant CTGF at a final concentration of 5 p-g/L The cells were collected at 72 h time points.Direct immunoiluorescence staining and immunohistochemistry were used to evaluate the E-cadherin,Vimentin,α-SMA and ERK2 in cells.Western-blotting was used to detect the E-cadherin,Vimentin and ERK2 protein expression.Boyden Chamber was used to detect the migration of tubular endothelium at 1 d,3 d and S d.Results There were less E-cadherin but more Vimentin expressed in cells of the experimental group.The presence of α-SMA was detected at 48 h with peak at 72 h in the cells of the experimental group.On the first day,the cellular migration in the two groups showed no difference.However,after 3 days,the transformed cells migrated surpassed the control group with peak at the 5th day [ (45.0±1.1) : (14.0±1.2),P < 0.05 ) ].Conclusion Connective tissue growth factor induces mesenchymal transformation of HK-2 cells,in which the ERK2 signaling pathway may play an important role.