去端肽胶原介导小干扰RNA抑制大鼠静脉移植物内膜增生

Objective To evaluate the efficacy of using small interfering RNA targeting TF as a therapy for vein graft failure. Methods External jugular vein to carotid artery interposition vein grafts, which were applied to a low flow condition, were made in 120 Sprague-Dawley rats weighing 260 to 300 g. These rats were randomly divided into 4 groups, 30 rats each group. Group A was atelocollagen-TF Stealth<'TM> Select RNAi group. Group B was atelocollagen-TF Stealth<'TM> RNAi group. Group C was atelocollagen group. Group D was control group. Small interfering RNA mixed with atelocollagen was administrated to the external wall of grafted veins. The TF protein expression of vein gratis was analyzed by Western blot at 1, 3, 7, 14, and 28 d postoperatively, and by immunochemistry at 3 d postoperatively. The proliferation index was determined at 14 d postoperatively. Neointimal hyperplasia was evaluated at 28 d postoperatively. BLOCK-iTTM fluorescent oligo was used to confirm its stability and successful transfer into the vein graft wall at 3 and 7 d postoperatively for another group (n = 12). Results Fluorescence of BLOCK-iT<'TM> fluorescent align could be detected in the graft wall even at 7 d postoperatively. Knockdown of the TF expression was achieved by perivascular application of siRNA using atelocollagen. Compared with control group, the intima thickness at 28 d after grafting was significantly reduced (P ＜ 0.05). This phenomenon was preceded by significant reduction of cell proliferation in siRNA-treated grafts at 14 d postoperatively (P ＜0.05). Conclusion The expression of TF in vein grafts can be effectively inhibited by specific siRNAs using a atelocoilagen-based nonviral delivery approach/n vivo, so that the neointimal thickening can be prevented.     

对付脚气办法多

脚癣俗称“脚气”，是由浅部致病真菌引起的，多发生在炎热潮湿的夏季。脚癣看上去不是什么大病。却奇痒无比让人倍感折磨。脚癣病人若不注意个人卫生，这病菌还会到处乱串．感染其它部位，如引起手癣、体癣和甲癣等。更有甚，这脚癣还可传染给他人。因此，有了脚癣可要早点治疗。     

葡萄糖的质量对大输液澄明度的影响

输液剂是供静脉滴注的注射液。输液治疗除能达到高效速效的目的外,从新陈代谢的角度来说,有预防或纠正体液紊乱,以及供给营养物质,促进组织修复,能为病人创造良好的治疗基础,从而提高治愈率。为此,对输液的质量要求就非常严格,除需确保无菌、无热原反应外,尚应保证药液的澄明和微粒减少至最低限度。影响输液的澄明度因素很多。一般多重     

在奎宁存在下四碘荧光素与新洁而灭缔合反应的研究

研究了在奎宁存在下四碘荧光素与新洁而灭的缔合反应。在pH 7.9～8.6范围内新浩而灭与四碘荧光素、奎宁形成稳定的三元离子缔合物(λ_max545 nm)。用1,2-二氯乙烷萃取时e=5.8×10⁴ 1·mol^-1·cm^-1。测出缔合物组成为1∶1∶1。研究了测定微量新浩而灭的条件和方法。2×10^-7～4×10^-6M新洁而灭符合Lambert-Beer定律,多数胺与生物碱不干扰测定。比较了在奎宁存在下17种酸性染料与新洁而灭的萃取—分光光度体系,确定荧光素类显色剂的效果优于类似结构的磺酞类显色剂。     

浙江省医药总公司召开“全省医药系统查处假药、劣药,加强质量管理工作会议”

浙江省医药系统查处假药、劣药,加强医药质量管理工作会议于1985年10月5日在杭州召开,历时六天。省委副书记、副省长吴敏达同志,省人大副主任商景才同志在会上作了重要指示。出席大会的有省府办公厅、省纪律检查委员会、省政协、省计经委、省检察院、省高级人民法院、省体改办、省编委、省公安、卫生、商业等十一个厅局,及     

高屈曲型与传统假体全膝置换对关节活动度影响的Meta分析

[目的]比较高屈曲型和传统假体进行全膝置换(totalkneearthrophsty,TKA)的术后关节活动度的差异.[方法]检索Medline、Embase、Cochranelibrary、CBM等,收集高屈曲型和传统假体TKA的对照试验,系统评价各研究质量,提取关节活动度的量化结果,采用RevMan5.0.18进行Meta分析.[结果]10个研究纳入Meta分析,共1124膝,其中高屈曲型假体组551膝,传统假体组573膝.统计结果显示:5个随机研究组中两组术后ROM无明显差异(P=0.05);非随机研究组中高屈曲的术后ROM略优于传统假体组(WMD=2.82°,95％CI[0.28,5.36],P＝0.03),但也少于5°,缺乏临床意义,并且该结论可能存在一定的发表性偏倚.[结论]目前的证据未能表明高屈曲型假体较传统假体更具提高术后膝ROM的优势.     

基因多态性与膝关节原发性骨性关节炎的相关研究进展

目前骨性关节炎(osteoarthritis,OA)的遗传学研究越来越受到关注,膝关节作为OA最早、最容易受累的关节之一,其研究价值尤为突出。随着对基因多态性的研究深入,越来越多的与膝OA相关的基因研究见诸报道,并证实了多种基因在膝关节原发性骨性关节炎的发生、发展中的重要作用,为膝关节     

晨尿尿蛋白／尿肌酐比值与24小时尿蛋白定量相关性分析

尿蛋白持续增多是肾脏损害的标志,24h尿蛋白定量测定是长期以来的“金指标”,但常常存在收集尿液繁杂、定时和定量不准确、部分尿样丢失、膀胱排空不完全、患者依从性差等问题,影响结果的准确性。为纠正此缺陷,美国肾脏病基金会改善肾脏疾病预后和生存质量的指南（NKFK／DOQI）推荐了尿蛋白／尿肌酐比值法。