燥湿和胃方对脾胃湿热证大鼠HPA轴、炎症因子及免疫功能的影响

目的 探讨燥湿和胃方对脾胃湿热证大鼠HPA轴、炎症因子及免疫功能的影响。方法 采用"环境因素+肥甘饮食+致炎因子"的方法复制脾胃湿热证动物模型。SPF级SD大鼠，♂，随机分为6组，即正常组，模型组，三九胃泰组，燥湿和胃方高、中、低剂量组（4，2，1 g·kg^-1），每组10只。采用放射免疫法检测促肾上腺皮质激素释放激素（corticotropin releasing homone，CRH）、促肾上腺皮质激素（adrenocorticotropic hormone，ACTH）、皮质醇（cortisol，Cor）的含量；采用免疫组化法检测大鼠胃黏膜组织中环氧合酶-2（cyclooxygenase，COX-2）表达水平；采用ELISA法检测肿瘤坏死因子-α（TNF-α）、白介素1β（IL-1β）、干扰素-γ（interferen-gamma，IFN-γ）、白介素4（IL-4）含量。结果 与正常组相比，模型组大鼠血清中CRH、ACTH、Cor、TNF-α、IL-1β、IFN-γ、IL-4、IFN-γ/IL-4值明显升高（P<0.05）。与模型组比较，燥湿和胃方各剂量组均显著降低大鼠血清中上述指标的含量（P<0.05）。与燥湿和胃方高剂量组相比，低剂量组大鼠血清中CRH、ACTH、Cor含量明显较高（P<0.05）；中剂量组大鼠血清CRH、ACTH、Cor含量稍高，但差异没有统计学意义，IL-1β、IL-4与其相当，差异没有统计学意义，TNF-α、COX-2的改善程度明显较弱（P<0.05），IFN-γ、IFN-γ/IL-4值均明显偏高（P<0.05）。结论 燥湿和胃方能有效改善脾胃湿热模型大鼠的疾病活动状态，该作用的发挥可能与调整HPA轴CRH、ACTH、Cor的含量有关，可能通过降低HPA轴相关指标，降低机体炎症反应，改善免疫状态，恢复免疫系统Th1/Th2的动态平衡。     

儿童专科医院护士执业环境测评与特征分析

目的 调查三级儿童专科医院护士执业环境感知现状,并分析其特征,从而能够进一步了解儿童专科医院护士执业环境,为改进儿科护士执业环境提供参考依据.方法 于2019年12月依托国家护理质量数据平台,应用"护士执业环境测评量表"对重庆市某三级儿童专科医院1013名护士进行整群抽样横断面调查.结果 护士执业环境测评平均值为77....     

基于高可靠性组织理论的儿科住院患者意外伤害事件管理方法的实施及成效

目的:引入高可靠性组织理论对儿科住院患者意外伤害不良事件进行规范化管理,降低儿科住院患者意外伤害事件发生率.方法:基于高可靠性组织理论构建儿科住院患者意外伤害不良事件管理体系并实施管理.结果:应用高可靠性组织理论对儿科住院患者意外伤害事件进行管理后,意外伤害的发生率降低0.061‰;儿科住院患者意外伤害预防措施落实率及...     

细胞色素P450 CYP1A1基因多态与鼻咽癌易感性的关联分析

Objective To investigate the association between CYP1A1 gene polymorphisms and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families through family-based association study. Methods A total of 457 Cantonese nuclear families, consisting of 2134 members, were recruited as subjects. Each family included two parents and at least one offspring with nasopharyngeal carcinoma. Two single nucleotide polymorphisms (SNP) in CYP1A1 named m1 (rs4646903) and m2 (rs1048943) , were genotyped by PCR-RFLP assay and verified by directly sequencing. The genotype data were analyzed with family-based association test (FBAT) software to check the linkage and association between the two genetic markers and susceptibility of nasopharyngeal carcinoma. Results FBAT analysis showed that the minor allele frequencies (MAF) of the two SN P were 0. 442 (C) and 0. 339 (G) respectively. For m1 polymorphism in CYP1A1 gene was not significantly associated with nasopharyngeal carcinoma in our study population whether stratified with VCA-IgA or not (without stratification : X2=2. 399, P=0. 301 ; with stratification : Iow-titer group (VCA-IgA<1 : 80), MAF=0. 457 (C), X2=1.221, P=0.543 ; high-titer group (VCA-IgA ≥1 : 80), MAF=0. 427 (C), X2=2. 832, P=0. 243). For m2 polymorphism, when VCA-IgA<1 : 80, the G allele showed decreased transmission under additive and dominant model (MAF=0. 347 (G) ; Zadditive=-2. 120,Padditive=0. 034;Zdominant=-2. 303,Pdominant=0.021)and a boundary P value was got with global statistic (X2=5. 394, P=0. 067). Haplotype TG (0. 057), constructed by ml and m2, might decrease nasophargneal carcinoma risk (Z=-2. 002,P=0. 045). A boundary P value was also got with global statistic (X2=7. 067 ,P=0. 070). Conclusion There was no statistical significance between ml polymorphism and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families. And this study showed that m2 polymorphism might associated with the decrease of nasopharyngeal carcinoma in Cantonese nuclear families.     

细胞色素P450 CYP1A1基因多态与鼻咽癌易感性的关联分析

Objective To investigate the association between CYP1A1 gene polymorphisms and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families through family-based association study. Methods A total of 457 Cantonese nuclear families, consisting of 2134 members, were recruited as subjects. Each family included two parents and at least one offspring with nasopharyngeal carcinoma. Two single nucleotide polymorphisms (SNP) in CYP1A1 named m1 (rs4646903) and m2 (rs1048943) , were genotyped by PCR-RFLP assay and verified by directly sequencing. The genotype data were analyzed with family-based association test (FBAT) software to check the linkage and association between the two genetic markers and susceptibility of nasopharyngeal carcinoma. Results FBAT analysis showed that the minor allele frequencies (MAF) of the two SN P were 0. 442 (C) and 0. 339 (G) respectively. For m1 polymorphism in CYP1A1 gene was not significantly associated with nasopharyngeal carcinoma in our study population whether stratified with VCA-IgA or not (without stratification : X2=2. 399, P=0. 301 ; with stratification : Iow-titer group (VCA-IgA<1 : 80), MAF=0. 457 (C), X2=1.221, P=0.543 ; high-titer group (VCA-IgA ≥1 : 80), MAF=0. 427 (C), X2=2. 832, P=0. 243). For m2 polymorphism, when VCA-IgA<1 : 80, the G allele showed decreased transmission under additive and dominant model (MAF=0. 347 (G) ; Zadditive=-2. 120,Padditive=0. 034;Zdominant=-2. 303,Pdominant=0.021)and a boundary P value was got with global statistic (X2=5. 394, P=0. 067). Haplotype TG (0. 057), constructed by ml and m2, might decrease nasophargneal carcinoma risk (Z=-2. 002,P=0. 045). A boundary P value was also got with global statistic (X2=7. 067 ,P=0. 070). Conclusion There was no statistical significance between ml polymorphism and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families. And this study showed that m2 polymorphism might associated with the decrease of nasopharyngeal carcinoma in Cantonese nuclear families.     

护理学本科健康评估课程思政建设的教学设计与实施

目的 探讨护理本科健康评估课程思政建设的教学设计的方法和效果。方法 选取2021年9月—2022年1月重庆医科大学护理本科二年级515名学生作为研究对象,明确课程思政目标,依托重庆医科大学课程思政资源库,集结课程相关思政元素,确定授课模式,通过多种教学方法实现思政元素的融入,同时辅以各类活动及竞赛将思政元素内化。通过量性评价与质性评价结合的方法明确思政教学效果。结果 5名重庆医科大学教学督导专家对健康评估课程思政的整体评价较好;所有学生对本课程教学改革的满意度为90%及以上;在整个课程思政过程中师生得到了共同成长。结论 健康评估课程思政教学改革的可取之处在于对思政资源库的建设,促进思政资源的优化与共享;选择适宜的教学方法;共筑“师生共同体”,促进师生全面发展。     

2019年聊城市流行性感冒流行病学特征及时空聚集性分析

目的 了解聊城市流行性感冒流行病学特征及时空聚集性特征,为流感的防控提供科学依据.方法 描述性流行病学方法 分析2019年全市流行性感冒疫情资料三间(人间、时间、空间)分布;使用SaTScanv9.6进行时空聚集性分析.结果 2019年聊城市报告流行性感冒病例10 160例,年发病率为167.26/10万.报告病例居前...     

手术室全员参与护理管理的做法与体会

成立行政组、质量控制组、教研组 ,实现了手术室全员参与的管理模式 ,从而使手术室的管理工作制度化、标准化、规范化 ,充分发掘了各级护理人员的潜能 ,增强了手术室管理的顺从性 ,提高了手术配合质量     

胸腔镜下心脏瓣膜置换术的护理

自1997年Chitwood首次成功完成胸腔镜下二尖瓣手术以来[1],胸腔镜瓣膜置换术在全球范围内得到蓬勃发展,被认为是体外循环问世以来心脏外科又一次重大的技术革命.胸腔镜下行心脏手术具有创伤小、出血少、疼痛轻、并发症少、恢复快、治疗费用低等优点[2],是心血管外科领域发展的一项很有前景的新技术[3].围术期的护理是提高手术成功的关键因素,本文就胸腔镜瓣膜置换手术适应证、术前护理、术后护理进行阐述.     

细胞色素P450 CYP1A1基因多态与鼻咽癌易感性的关联分析

Objective To investigate the association between CYP1A1 gene polymorphisms and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families through family-based association study. Methods A total of 457 Cantonese nuclear families, consisting of 2134 members, were recruited as subjects. Each family included two parents and at least one offspring with nasopharyngeal carcinoma. Two single nucleotide polymorphisms (SNP) in CYP1A1 named m1 (rs4646903) and m2 (rs1048943) , were genotyped by PCR-RFLP assay and verified by directly sequencing. The genotype data were analyzed with family-based association test (FBAT) software to check the linkage and association between the two genetic markers and susceptibility of nasopharyngeal carcinoma. Results FBAT analysis showed that the minor allele frequencies (MAF) of the two SN P were 0. 442 (C) and 0. 339 (G) respectively. For m1 polymorphism in CYP1A1 gene was not significantly associated with nasopharyngeal carcinoma in our study population whether stratified with VCA-IgA or not (without stratification : X2=2. 399, P=0. 301 ; with stratification : Iow-titer group (VCA-IgA<1 : 80), MAF=0. 457 (C), X2=1.221, P=0.543 ; high-titer group (VCA-IgA ≥1 : 80), MAF=0. 427 (C), X2=2. 832, P=0. 243). For m2 polymorphism, when VCA-IgA<1 : 80, the G allele showed decreased transmission under additive and dominant model (MAF=0. 347 (G) ; Zadditive=-2. 120,Padditive=0. 034;Zdominant=-2. 303,Pdominant=0.021)and a boundary P value was got with global statistic (X2=5. 394, P=0. 067). Haplotype TG (0. 057), constructed by ml and m2, might decrease nasophargneal carcinoma risk (Z=-2. 002,P=0. 045). A boundary P value was also got with global statistic (X2=7. 067 ,P=0. 070). Conclusion There was no statistical significance between ml polymorphism and susceptibility of nasopharyngeal carcinoma in Cantonese nuclear families. And this study showed that m2 polymorphism might associated with the decrease of nasopharyngeal carcinoma in Cantonese nuclear families.