Site‐specific accumulation and dynamic change of flavonoids in Apocyni Veneti Folium

Site‐specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin‐HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured using HPLC method. LSCM observation showed that flavonoids are accumulated in cuticle of epidermal cells and vessel walls, especially in protoplasts and nucleolus of the collenchyma cells and the epidermal cells. Catechins are localized in the palisade parenchyma cells and vessel walls, particularly in the laticifers found in the phloem. On the basis of the difference of the maximal emission wavelength between quercetin and kaempferol derivatives which have fluorescence behavior by appropriate treatment, kaempferol and its derivatives are localized exclusively in the cuticle. Results showed that the content of astragalin in Apocyni Veneti Folium from different parts revealed the decreasing trend, while hyperin and isoquercitrin were higher in June and July analyzed by HPLC. In summary, the site‐specific accumulation of flavonoids in Apocyni Veneti Folium can be determined by LSCM and vanillin‐HCl staining. The contents of flavonoids in Apocyni Veneti Folium are correlated with harvest times and growth parts.     

基于径向基网络的变速箱载荷识别研究

提出了一种基于径向基网络的识别变速箱载荷的新方法,该方法应用径向基函数网络建立了箱体表面振动响应信号与轴承激励之问的非参数映射模型。通过变速箱在随机激励下的载荷识别结果表明,这种利用径向基网络识别变速箱载荷的方法是可行的,并且具有识别精度高、运算速度快及稳定性好等优点。     

高分辨率傅里叶变换透镜

在研究傅里叶变换透镜理论的基础上,根据某微粒子观测实验的要求和几何光学像差理论,得出了高分辨率傅里叶透镜的初始参数,分析了系统的消像差方法,并应用ZEMAX软件设计了满足要求的工作波长为532 nm的傅里叶变换透镜。在光机结构设计中,为减少外界环境对成像质量的影响,在频谱面处设计了光阑和低真空腔。工程分析和实验结果表明,抽真空引起的光学间距变化对该系统的成像质量基本没有影响,用目视显微镜法检测系统的分辨率为2.81 μm。该系统可以保持12 h的低真空(100 Pa以下),并且已成功应用于某微粒子观测实验。     

测量微悬臂梁曲率的相移显微干涉法

提出了一种基于显微干涉和有限差分法在微悬臂梁上实现曲率精确测量的方法.该方法将使用相移显微干涉法测得的微悬臂梁表面弯曲信息与用有限差分法解析的弯曲量进行对比,再运用拟牛顿算法或最小二乘法得到曲率的最佳匹配值.实验结果表明:使用该方法可获得弯曲量测量值和解析值之间的均方根差值在1.5 nm以内的精确曲率值,并且一定的像素偏移带来的误差对曲率测量的结果影响很小.由于方法保留了光学干涉法高分辨率及高精度等优点,并考虑了非理想边界条件的影响,在MEMS残余应力和应力梯度测量中具有较大实用价值.     

基于MEMS的人工视网膜微电极阵列设计仿真

本文介绍的是一种基于微机电系统（MEMS）技术的表面型人工视网膜微电极阵列设计与仿真。电脉冲通过微电极刺激视网膜神经细胞,在大脑皮层视觉区域引起对应的特征电位反应,部分恢复患者的视觉。为研究表面型人工视网膜微电极阵列在人眼压力环境下的位移,特别设计了多种微电极阵列,进行了应力仿真。     

定向斜井套管下入摩阻探讨

套管下入过程中,套管与井壁的摩阻可能会造成套管下入困难,影响油井的正常生产。因此,为高效开发油田,对套管下入摩阻问题进行分析,如果能够事先预测套管下入遇阻位置,并提出防治对策,有着重要意义。定向斜井井壁的弯曲和下入过程中套管柱的变形和受力是力学计算的难点,考虑大斜度井井身参数的实际情况,借用修改的别斯良克公式,由实测大钩载荷反演分段摩擦系数,并对某地区的一口井进行了试算。     

硅微机械扭转微镜的研究进展

硅微机械扭转微镜在国防和民用领域都有着重要的应用.根据致动机理的不同,从压电驱动方式、电磁驱动方式、电热驱动方式和静电驱动方式等方面系统地阐述了硅微机械扭转微镜的研究现状与发展方向,同时重点讨论了硅微机械扭转微镜在医疗成像和生物技术等领域中的具体应用.     

Investigation on infrared laser desorption of solid matrix using scanning electron microscope and fast photography

Infrared light from a pulsed optical parametric oscillator laser system was used to irradiate succinic acid (SA), a usual solid matrix used in matrix‐assisted laser desorption ionization, under vacuum. Ablated SA particles were trapped using a silica plate mounted 3.0 mm above and parallel to the sample surface. The morphology and particle size of ablated particles at different laser fluences were investigated using a scanning electron microscope (SEM). The dynamics of plume propagation for SA desorption process was studied with fast photography at atmospheric pressure. Plume expanding at 1.12 J/cm² laser fluence was recorded using a high‐speed CMOS camera and corresponding propagation distance was measured. The solid matrix desorption was driven by phase explosion according to plume model fitting, which was consistent with the results of SEM. Microsc. Res. Tech. 76:744–750, 2013. © 2013 Wiley Periodicals, Inc.     

江阴大跨越输电塔模型试验研究

本文基于江阴大跨越输电塔模型试验工作 ,对试验模型设计与制作、加载及反力装置的设计、试验工况及测点布置等进行了研究。结合塔架模型试验和高强度螺栓受剪连接试验结果 ,验证了大跨越的设计是安全可靠的。文中还对塔架计算模型、杆件次应力、高强度螺栓群连接极限承载力等问题进行了讨论 ,并得出一些有益结论供工程设计参考。     

干熄焦节能效果及对焦炭质量的影响分析

针对干熄焦工艺特点，评价节能和环保效果，分析了焦炭质量变化机理和干熄焦在焦化行业的应用前景。