Propionic acid-metabisulphite-Schiff reagent for quick Feulgen staining

This communication presents a method for the preparation of Schiff reagent in which N hydrochloric acid has been replaced by a low concentration of propionic acid. The result of using such a Schiff reagent indicates that the dye-reagent thus prepared is extrafast in action on acid-hydrolysed mammalian tissue sections. The intensity of nuclear colouration is also increased considerably, since the pH of the dye-reagent is 6.0. It is, therefore advocated that this newly developed Schiff reagent be used at 5 degrees C.     

Preparation of Schiff-type dye-SO2 reagents with phosphoric acid and their cytochemical properties

A new method employing phosphoric acid in place of hydrochloric acid for the preparation of azure A-SO2 and toluidine blue O-SO2 has been described in this communication. It has been found that o-phosphoric acid in a weak concentration can be a suitable substitute for hydrochloric acid in the preparation of these dye-SO2 reagents. These dye-reagents produce excellent blue nuclei in human uterine tumour. Toluidine blue O-SO2 fortified with an amino aicd, glycine also produces excellent blue chromosomes in the squash preparations of grasshopper testes fixed in acetic acid-alcohol. The shelf-life of these two dye-reagents has also been presented herein.     

Characterizing and reproducing working set size dynamics

A characterization of program referencing dynamics based on the temporal behavior of memory demand (as represented by the working set size of a program for a given window size) is proposed. A deterministic generative model which produces a references string having a given dynamic characterization is then presented, and its practical implementation is discussed. An experimental study of the accuracy and the viability of such a model is performed, together with a theoretical and empirical investigation of the feasibility of constructing a synthetic program which produces an approximation to that artificial string, thereby exhibiting the given dynamic behavior. The results for working-set-like environments with window sizes larger than or equal to that used in the generation of the artificial string are satisfactory, but those for other types of memory policies reveal that improvements to the string generation algorithm or different characterizations are needed.     

Wire coating by withdrawal from a bath of power law fluid

An analysis for withdrawal of cylinders from power law fluids has been presented for predicting the withdrawal speed for a desired film thickness. The theory has been compared with the previously published photographic and flow rate data of a low viscous Newtonian fluid for three values of N_GO and shows close agreement up to N_CA = 0.7. Comparison of the theory with the published experimental data on only one essentially inelastic fluid (n = 0.73) shows close agreement up to N_CA = 0.3. The disagreement at higher speeds is attributable to shear thinning of polymeric solutions.     

Nonlinear programming solutions for controlling the vibration pattern of stretched strings

The problem of controlling the vibration pattern of a driven string is considered. The basic question dealt with here is to find the control forces which reduce the energy of vibration of a driven string over a prescribed portion of its length while maintaining the energy outside that length above a desired value. The criterion of keeping the response outside the region of energy reduction as close to the original response as possible is introduced as an additional constraint. The slack unconstrained minimization technique (SLUMT) has been successfully applied to solve the above problem. The effect of varying the phase of the control forces (which results in a six-variable control problem) is then studied. The nonlinear programming techniques which have been effectively used to handle problems involving many variables and constraints therefore offer a powerful tool for the solution of vibration control problems.     

In situ demonstration of DNA with Schiff-type dyes prepared with meta-phosphoric or tartaric acid

A new method for the preparation of azure A-SO2 and safranine-SO2 For use in Feulgen procedure has been described herein. The method involves the use of m-phosphoric acid or tartaric acid in place of N HCl in the preparation of these eye-reagents which exhibit enhanced pH producing increased staining intensity of the nuclei as compared with those of the controls, prepared with N HCl. Possible explanation for the increased staining intensity as well as the reason for the shorter shelf-life of these eye-reagents have been offered.     

Alumina Supported Co–K–Mo Based Catalytic Material for Diesel Soot Oxidation

Alumina supported Co–K–Mo based mixed metal oxide type catalytic materials have been prepared by co-impregnation. These catalysts show excellent activity for carbon as well as diesel soot oxidation, which could be due to the redox properties of Mo and Co as well as to a synergistic effect of molybdenum, cobalt, and K contents. The catalyst containing 5 wt% molybdenum shows a lowering of carbon oxidation by about 190 °C under loose contact conditions as compared to the non-catalyzed reaction, as well as to bare alumina. Characterization studies suggest a composite nature of these materials, while thermal stability investigations confirm the stable nature. The selected catalyst has been studied by XPS, however, it is difficult to conclude which are the important factors contributing to the catalytic activity. It appears to be a synergistic effect of Co, K, and Mo components as these catalysts show much improved activity as compared to the individual components in supported and unsupported forms.