Housing quality and resilience in New Zealand

There is extensive research on the negative health impacts of poor housing quality. However, little is known about the potential health benefits of high-quality housing in poor neighbourhoods. Neighbourhoods with unexpectedly good health outcomes despite high levels of deprivation have been deemed resilient places and housing quality in these areas may be a contributor to this resilience. This study aimed to evaluate whether an indicator of neighbourhood housing quality was associated with a previously quantified resilience index (RINZ) in New Zealand. It was found that areas with high housing quality tended to have higher median income, greater proportions of partnered people and shorter-term residents, and very low proportions of Māori. A positive association was found between housing quality and resilience, after adjustment for deprivation. There was no indication of differences by heterogeneity in housing quality within the aggregate unit of analysis. These findings pose the hypothesis that improving housing quality in similarly deprived areas that have poor health outcomes could potentially boost health. To extend this understanding, further development of a more sophisticated housing quality indicator is recommended.     

Somatotropic axis and concentrate supplementation in grazing dairy cows of genetically diverse origin

Supplementing pasture-fed dairy cows with concentrates in early lactation was hypothesized to result in an earlier postpartum recoupling of the somatotropic axis in New Zealand (NZ)-type Holstein-Friesian dairy cows than in North American (NA)-type cows. To test this hypothesis, NA (n = 30) and NZ (n = 30) cows were allocated to 1 of 3 supplementation strategies (0, 3, or 6 kg of dry matter concentrate/d) for the first 12 wk of lactation in a completely randomized design and a 2 × 3 factorial arrangement. Production traits and characteristics of the somatotropic axis were studied at phenotypic, hormonal, and gene expression levels. Milk production and plasma metabolite concentrations were measured weekly, and liver was biopsied in wk 1, 4, 8, and 12 postcalving. North American cows produced more milk and displayed a larger degree of somatotropic axis uncoupling than did NZ cows. This was evident in strain differences in body condition score, blood growth hormone, and insulin-like growth factor-1 concentrations, and hepatic expression of growth hormone receptor-1a. No strain × diet interactions were observed for any characteristic of the somatotropic axis at either the blood metabolite or gene expression level; however, blood insulin concentrations during wk 7 to 11 postpartum increased with concentrate supplementation in NZ but not NA cows. These results demonstrate that feeding supplements does not result in an earlier recoupling of the somatotropic axis; however, the greater blood insulin concentrations with concentrate feeding in NZ cows from wk 7 may result in an earlier recoupling in this genetic strain, after the period investigated in this study. Further research is required to understand differences in insulin control between these genetic strains.     

“二奢”更香

真金换旧爱,二手奢侈品为啥这么火?近期,部分奢侈品品牌开启新一轮涨价热潮,奢侈品门店外的队伍越排越长,门店内配货的盒子越垒越高,与此相映成趣的是疫情下"叫卖声"火热的二手奢侈直播间。包括寺库、只二、胖虎、红布林及妃鱼在内的国内二手奢侈品平台均入局直播电商,实体门店顾客群更是在线下人流受阻的情况下积极拥抱线上直播.     

Hepatotoxicity of Drugs Used in Multiple Sclerosis,Diagnostic Challenge,and the Role of HLA Genotype Susceptibility

Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system and the association with other autoimmune diseases is well-documented. There are many therapeutic options for the treatment of MS. Most of the available drugs cause drug-induced liver injury (DILI) to variable extents with heterogeneous clinical and biological manifestations, including liver injury with or without signs of hypersensitivity and autoimmunity. The diagnosis of DILI may be particularly difficult because MS is frequently associated with idiopathic autoimmune hepatitis. Recent advances suggest that MS and immune-mediated DILI could be promoted by genetic factors, including HLA genotype. In addition, some of these drugs may promote hepatitis B virus reactivation. This review explores the potential hepatotoxicity of drugs used to treat MS and the criteria to distinguish DILI from idiopathic autoimmune hepatitis associated with MS. The role of susceptible genes both promoting MS and causing the hepatotoxicity of the drug used for MS treatment is also discussed.     

Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression

The LH surge initiates the luteinization of preovulatory follicles and causes hormonal and structural changes that ultimately lead to ovulation and the formation of corpora lutea. The objective of the study was to examine gene expression in ovarian follicles (n = 11) collected from pigs (Sus scrofa domestica) approaching estrus (estrogenic preovulatory follicle; n = 6 follicles from two sows) and in ovarian follicles collected from pigs on the second day of estrus (preovulatory follicles that were luteinized but had not ovulated; n = 5 follicles from two sows). The follicular status within each follicle was confirmed by follicular fluid analyses of estradiol and progesterone ratios. Microarrays were made from expressed sequence tags that were isolated from cDNA libraries of porcine ovary. Gene expression was measured by hybridization of fluorescently labeled cDNA (preovulatory estrogenic or -luteinized) to the microarray. Microarray analyses detected 107 and 43 genes whose expression was decreased or increased (respectively) during the transition from preovulatory estrogenic to -luteinized (P<0.01). Cells within preovulatory estrogenic follicles had a gene-expression profile of proliferative and metabolically active cells that were responding to oxidative stress. Cells within preovulatory luteinized follicles had a gene-expression profile of nonproliferative and migratory cells with angiogenic properties. Approximately, 40% of the discovered genes had unknown function.     

Phospholipid bilayer coatings for the separation of proteins in capillary electrophoresis

The double-chained, zwitterionic phospholipid 1,2-dilauroyl-sn-phosphatidylcholine (DLPC, C12) was investigated for its use as a wall coating for the prevention of protein adsorption in capillary electrophoresis. DLPC forms a semipermanent coating at the capillary wall, which allows excess phospholipid to be removed from the capillary prior to electrophoretic separation. A DLPC-coated capillary allowed for the separation of both cationic and anionic proteins with efficiencies as high as 1.4 million plates/m. Migration time reproducibility was less than 1.3% RSD from run to run and less than 4.0% RSD from day to day. Protein recovery was as high as 93%. Cationic and anionic proteins could be separated over a pH range of 3-10, all yielding good efficiencies (N up to 1 million plates/m). The chain length of the phospholipid affected the performance of the wall coating. The C10 analogue of DLPC (DDPC) did not form a coating on the capillary wall while the C14 analogue of DLPC (DMPC) formed a stable coating that prevented protein adsorption to the same extent as its C12 counterpart.     

Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury

qRT-PCR still remains the most widely used method for quantifying gene expression levels, although newer technologies such as next generation sequencing are becoming increasingly popular. A critical, yet often underappreciated, problem when analysing qRT-PCR data is the selection of suitable reference genes. This problem is compounded in situations where up to 25% of all genes may change (e.g., due to leukocyte invasion), as is typically the case in ARDS. Here, we examined 11 widely used reference genes for their suitability in commonly used models of acute lung injury (ALI): ventilator-induced lung injury (VILI), in vivo and ex vivo, lipopolysaccharide plus mechanical ventilation (MV), and hydrochloric acid plus MV. The stability of reference gene expression was determined using the NormFinder, BestKeeper, and geNorm algorithms. We then proceeded with the geNorm results because this is the only algorithm that provides the number of reference genes required to achieve normalisation. We chose interleukin-6 (Il-6) and C-X-C motif ligand 1 (Cxcl-1) as the genes of interest to analyse and demonstrate the impact of inappropriate normalisation. Reference gene stability differed between the ALI models and even within the subgroup of VILI models, no common reference gene index (RGI) could be determined. NormFinder, BestKeeper, and geNorm produced slightly different, but comparable results. Inappropriate normalisation of Il-6 and Cxcl1 gene expression resulted in significant misinterpretation in all four ALI settings. In conclusion, choosing an inappropriate normalisation strategy can introduce different kinds of bias such as gain or loss as well as under- or overestimation of effects, affecting the interpretation of gene expression data.     

Training issues.

This interesting set of articles, taken as a group, suggests that we have come a long way toward the integration of family therapy into the training of psychologists. The question is no longer "Should psychologists be trained in family therapy?" but "How can such training best be accomplished, in what settings, with what approaches, and how related to other elements of the graduate or postdoctoral program?" The articles by Ribordy (see record 1989-13401-001) and by Lebow (see record 1989-13365-001) address the central issues in training from different vantages (the family institute versus the academic program) but converge on many points. I will comment mainly on these two articles, but first I want to take note of some of the ideas raised by Berger and Jurkovic (see record 1989-12730-001). They describe and persuasively illustrate a contextual approach to family work that emphasizes the varying settings in which therapy may occur. Thus they are concerned with a "supersystem" that incorporates these extra therapeutic systems as well as clinicians and family. It will be no great surprise to the readers of this Journal that many of these same points emerge from Ribordy's and Lebow's more general discussions of training issues. They explore the separate territories of the academic graduate program and the family institute as settings for training and ably outline the advantages and disadvantages of each. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A Simple Technique for Measuring Expansion of Methylcellulose Films in Water

A simple and easy to assemble apparatus for the determination of expansion of methylcellulose f ilms is described. The technique involved essentially the projection of the image of the film on to a screen and the measurment of the expansion of the film with time. From statistical analyses of the data obtained for film expansion, the method was found to be reliable. The size, shape and amount of methylcellulose/area of film were considered in the assessment of the technique.     

Adult attachment and the perception of facial expression of emotion.

Adult attachment orientation has been associated with specific patterns of emotion regulation. The present research examined the effects of attachment orientation on the perceptual processing of emotional stimuli. Experimental participants played computerized movies of faces that expressed happiness, sadness, and anger. Over the course of the movies, the facial expressions became neutral. Participants reported the frame at which the initial expression no longer appeared on the face. Under conditions of no distress (Study 1), fearfully attached individuals saw the offset of both happiness and anger earlier, and preoccupied and dismissive individuals later, than the securely attached individuals. Under conditions of distress (Study 2), insecurely attached individuals perceived the offset of negative facial expressions as occurring later than did the secure individuals, and fearfully attached individuals saw the offset later than either of the other insecure groups. The mechanisms underlying the effects are considered. (PsycINFO Database Record (c) 2010 APA, all rights reserved)