NanoPaint: A Tool for Rapid and Dynamic Imaging of Membrane Structural Plasticity at the Nanoscale

Single‐particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano‐resolved population dynamics of QDs is exploited to reconstruct the topography and structural changes of the cell membrane surface with high temporal and spatial resolution. For this proof‐of‐concept study, bright, small, and stable biofunctional QD nanoconstructs are utilized recognizing the endogenous neuronal cannabinoid receptor 1, a highly expressed and fast‐diffusing membrane protein, together with a commercial point‐localization microscope. Rapid QD diffusion on the axonal plasma membrane of cultured hippocampal neurons allows precise reconstruction of the membrane surface in less than 1 min with a spatial resolution of tens of nanometers. Access of the QD nanoconstructs to the synaptic cleft enables rapid 3D topological reconstruction of the entire presynaptic component. Successful reconstruction of membrane nano‐topology and deformation at the second time‐scale is also demonstrated for HEK293 cell filopodia and axons. Named “nanoPaint,” this super‐resolution imaging technique amenable to any endogenous transmembrane target represents a versatile platform to rapidly and accurately reconstruct the cell membrane nano‐topography, thereby enabling the study of the rapid dynamic phenomena involved in neuronal membrane plasticity.     

Localization and speciation of chromium in subterranean clover using XRF, XANES, and EPR spectroscopy

Optimization of phytoremediation and assessment of potential health hazards from metals in the environment requires an understanding of absorption, localization, and transport of the target metal by plants. The objectives of this study were to localize Cr and determine the oxidation state and possible complexation mode of Cr in intact plant tissue by means of XANES, synchrotron XRF microprobe spectroscopy, and EPR spectroscopy. Subterranean clover (Trifolium brachycalycinum) was grown hydroponically with Cr(VI) (0.04-2.0 mmol L(-1)) and compared with plants grown without Cr and with inorganic Cr(III) and various Cr(III)-organic sources. The uptake, translocation, and form of Cr in the plant were dependent on the form and concentration of supplied Cr. Chromium was found predominately in the +3 oxidation state, regardless of the Cr source supplied to the plant, though at high Cr(VI) treatment concentrations, Cr(VI) and Cr(V) were also observed. At low Cr(VI) concentrations, the plant effectively reduced the toxic Cr(VI) to less toxic Cr(III), which was observed both as a Cr(III) hydroxide phase at the roots and as a Cr(III)-organic complex in the roots and shoots. At low Cr(VI) treatment concentrations, Cr in the leaves was observed predominately around the leaf margins, while at higher concentrations Cr was accumulated at leaf veins.     

Synergy of combining sonolysis and photocatalysis in the degradation and mineralization of chlorinated aromatic compounds

Merits of using advanced oxidation processes such as sonolysis and photocatalysis as well as a combination of the two have been explored using model herbicides such as 2,4-dichlorophenoxy acetic acid and 2,4-dichlorophenoxypropionic acid and the chlorinated phenols 2,4-dichlorophenol and 2,4,6-trichlorophenol. Whereas sonolysis is quite effective in the initial degradation of chlorinated aromatic molecules, complete mineralization is difficult to achieve. Photocatalysis is selective toward the degradation of polar compounds but causes the build up of undesirable chemical intermediates. In contrast to sonolytic degradation, photocatalysis is very effective toward achieving complete mineralization. By simultaneously carrying out high-frequency sonolysis and photocatalysis we have succeeded in achieving faster and complete mineralization with no build up of toxic intermediates even at very low catalyst loadings. The synergy of combining the two advanced oxidation processes is discussed.     

Stability and bioaccessibility of different forms of carotenoids and vitamin A during in vitro digestion

Vitamin A deficiency is a public health issue in developing countries and promoting dietary carotenoids as precursors is a promising strategy. However, carotenoids present in numerous fruits and vegetables are unstable and poorly bioaccessible. This study evaluated these two parameters during in vitro digestion of carotenoids and retinoids from carrot juice, raw and cooked spinach, micronutrient-fortified flour and standards without food matrix. Standards were unstable whereas vitamin A from fortified flour and native food carotenoids were generally better protected by the food matrix (30–100% remaining versus 7–30% for standards). Hydrothermal cooking did not influence spinach carotenoid digestive stability but decreased their contents, phenomenon compensated by a significantly better micellarisation from 15-fold for β-carotene to 72-fold for lutein. Finally, carrot juice provided the greatest amount of bioaccessible provitamin A with 1850 μg/100 g dry matter (DM) versus 790 and 80 μg/100 g DM in cooked and raw spinach, respectively.     

Dark-capacitance transients in MIS tunnel diodes

Dark-capacitance transients in MIS tunnel diodes switched from accumulation to deep depletion depend on the interface state occupancy. This time dependent function is derived and its behavior described for electron emission and electron-hole pair generation with tunneling and thermally controlled occupancies. A new method of determining the tunneling relaxation time constant τ_T from the electron emission transient at low temperature is described. Measurements of MIS tunnel diodes with oxide thickness d_ox = 17 to 80 Å agree with theory and the resultant values of τ_T compare with previously reported results from photocapacitance[2].     

Active Packaging Applications for Food

The traditional role of food packaging is continuing to evolve in response to changing market needs. Current drivers such as consumer's demand for safer, “healthier,” and higher‐quality foods, ideally with a long shelf‐life; the demand for convenient and transparent packaging, and the preference for more sustainable packaging materials, have led to the development of new packaging technologies, such as active packaging (AP). As defined in the European regulation (EC) No 450/2009, AP systems are designed to “deliberately incorporate components that would release or absorb substances into or from the packaged food or the environment surrounding the food.” Active packaging materials are thereby “intended to extend the shelf‐life or to maintain or improve the condition of packaged food.” Although extensive research on AP technologies is being undertaken, many of these technologies have not yet been implemented successfully in commercial food packaging systems. Broad communication of their benefits in food product applications will facilitate the successful development and market introduction. In this review, an overview of AP technologies, such as antimicrobial, antioxidant or carbon dioxide‐releasing systems, and systems absorbing oxygen, moisture or ethylene, is provided, and, in particular, scientific publications illustrating the benefits of such technologies for specific food products are reviewed. Furthermore, the challenges in applying such AP technologies to food systems and the anticipated direction of future developments are discussed. This review will provide food and packaging scientists with a thorough understanding of the benefits of AP technologies when applied to specific foods and hence can assist in accelerating commercial adoption.     

The development of an in-process surface roughness adaptive control system in end milling operations

An in-process surface roughness adaptive control (ISRAC) system in end milling operations was researched and developed. A multiple regression algorithm was employed to establish two subsystems: the in-process surface roughness evaluation (ISRE) subsystem and the in-process adaptive parameter control (IAPC) subsystem. These systems included not only machine cutting parameters such as feed rate, spindle speed, and depth of cut, but also cutting force signals detected by a dynamometer sensor. The multiple-regression-based ISRE subsystem predicted surface roughness during the finish cutting process with an accuracy of 91.5%. The integration of the two subsystems led to the ISRAC system. The testing resulted in a 100% success rate for adaptive control, proving that this proposed system could be implemented to adaptively control surface roughness during milling operations. This research suggests that multiple linear regression used in this study was straightforward and effective for in-process adaptive control.     

Bulk nanocrystalline aluminum 5083 alloy fabricated by a novel technique: Cryomilling and spark plasma sintering

Dense, bulk nanocrystalline aluminum 5083 alloy was fabricatedvia a combined technique: cryomilling (mechanical milling at cryogenic temperature) to achieve the nanocrystalline Al 5083 powder and spark plasma sintering (SPS) to consolidate the cryomilled powder. The results of X-ray diffraction analysis indicate that the average grain size in the SPS consolidated material is 51 nm, one of the smallest grain sizes ever reported in bulk Al alloys produced by powder metallurgy derived methods. In contrast, transmission electron microscopy (TEM) analysis revealed a bimodal grain size distribution, with an average grain size of 47 nm in the fine-grained regions and approximately 300 nm in the coarse-grained regions. Nanoindentation was used to evaluate the mechanical properties and the uniformity of the consolidated nanocrystalline Al 5083. The hardness of the material is greatly improved over that of the conventional equivalent, due to the fine grain size. The mechanisms for spark plasma sintering and the microstructural evolution are discussed on the basis of the experimental findings.