Cloning and characterization of the Inc A/C plasmid RA1 replicon

The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group.     

Aortic valve papillary fibroelastoma. A diagnosis by transthoracic echocardiography

Cardiac papillary fibroelastomas are unusual, frond-like growths typically found on cardiac valves, diagnosed incidentally on autopsy or cardiac surgery, but rarely during life. We report a rare case of an aortic valve papillary fibroelastoma detected by transthoracic echocardiography and confirmed by histologic study.     

Predictive equation for acquisition of hepatitis B in hospital workers in a general hospital

A cross-sectional study was performed to obtain risk factors for hepatitis B disease, HBsAg carriers and immunised personnel, among 2470 workers in a general hospital in Madrid, Spain. The data obtained were analyzed with multiple logistic regression to obtain beta coefficients for variables. The results of the analysis show that being a nurse or being regularly exposed to blood are the most important risk factors for hepatitis B acquisition. The length of time working at the same job activity was also a risk factor. The resulting beta coefficients allow the construction for a hepatitis non-immunised, HBsAg carrier and immunised HBV status, which can select subjects for a hepatitis B vaccination program.     

Methodological issues in outcome studies of school-based interventions for the prevention of adolescent depression.

Several methodological and ethical issues are addressed in the context of 3 related school-based studies of the primary and targeted prevention of depressive symptoms and disorder in high school adolescents. These issues include obtaining S consent and the protection of confidentiality, minimizing attrition over long-term follow-up periods, the "unit of assignment" issue common to most school-based research, and ensuring therapist fidelity to the intervention protocol. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The evolution of custom microarray manufacture

Given the enormous size of the genome and that there are potentially many other types of measurements we need to do to understand it, it has become necessary to pick and choose one's targets to measure because it is still impossible to evaluate the entire genome all at once. What has emerged is a need to have rapidly customizable microarrays. There are two dominant methods to accomplish custom microarray synthesis, Affymetrix-like microarrays manufactured using light projection rather than semiconductor-like masks used by Affymetrix to mass manufacture their GeneChip/sup TM/ arrays now, or the ink-jet printing method employed by Agilent. The manufacture of these custom Affymetrix-like microarrays can now be done on a digital optical chemistry (DOC) machine developed at the University of Texas Southwestern Medical Center, and this method offers much higher feature numbers and feature density than is possible with ink-jet printed arrays. On a microarray, each feature contains a single genetic measurement. The initial DOC prototype has been described in several publications, but that has now led to a second-generation machine. This machine reliably produces a number of arrays daily, has been deployed against a number of biomedical questions, is being used in new ways and has also led to a number of spin-off technologies.     

A comparative analysis of fuzzy logic and PI speed control in high-performance AC drives using experimental approach

One of the frequently discussed applications of artificial intelligence in motion control is the replacement of a standard proportional plus integral (PI) speed controller with a fuzzy logic (FL) speed controller. Regardless of all the work, it appears that a thorough comparison of the drive behavior under PI and FL speed control is still missing. This paper attempts to fill in this gap, by providing an in-depth comparison of operation of a vector-controlled permanent-magnet synchronous motor, using at first an experimental rig. Speed responses, obtained under PI and FL speed control, are recorded and compared for a variety of operating conditions. The transients studied include response to a large step speed command from standstill with nominal inertia and an increased inertia, response to small step speed reference change, and response to step load torque application. The transient behavior is examined for various initial speed settings, so that a thorough comparison is enabled. Experimental results are further supplemented with a set of simulation results, obtained using a different permanent-magnet machine and a different FL controller. Better generalization of the results is enabled in this way. It is shown that superiority of the FL speed control is less pronounced than it is often portrayed in the literature on the basis of limited comparisons. Indeed, in a number of cases, PI speed control provides a superior speed response.     

双塔脱丙烷工艺技术

对扬子乙烯装置双塔脱丙烷系统低压塔液泛、高压塔再沸器堵塞等问题进行了技术分析,提出了工艺流程修改方案,完善了双塔脱丙烷技术,对同类装置有参考价值。