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91.
92.
Fernando Bartolomé Úrsula Muñoz Noemí Esteras Carolina Alquezar Andrea Collado Félix Bermejo-Pareja Ángeles Martín-Requero 《Cellular and molecular life sciences : CMLS》2010,67(24):4257-4268
Statins may exert beneficial effects on Alzheimer’s disease (AD) patients. Based on the antineoplastic and apoptotic effects
of statins in a number of cell types, we hypothesized that statins may be able to protect neurons by controlling the regulation
of cell cycle and/or apoptosis. A growing body of evidence indicates that neurodegeneration involves the cell-cycle activation
in postmitotic neurons. Failure of cell-cycle control is not restricted to neurons in AD patients, but occurs in peripheral
cells as well. For these reasons, we studied the role of simvastatin (SIM) on cell survival/death in lymphoblasts from AD
patients. We report here that SIM induces apoptosis in AD lymphoblasts deprived of serum. SIM interacts with PI3K/Akt and
ERK1/2 signaling pathways thereby decreasing the serum withdrawal-enhanced levels of the CDK inhibitor p21Cip1 (p21) and restoring the vulnerability of AD cells to trophic factor deprivation. 相似文献
93.
Darío Acuña-Castroviejo Germaine Escames Carmen Venegas María E. Díaz-Casado Elena Lima-Cabello Luis C. López Sergio Rosales-Corral Dun-Xian Tan Russel J. Reiter 《Cellular and molecular life sciences : CMLS》2014,71(16):2997-3025
Endogenous melatonin is synthesized from tryptophan via 5-hydroxytryptamine. It is considered an indoleamine from a biochemical point of view because the melatonin molecule contains a substituted indolic ring with an amino group. The circadian production of melatonin by the pineal gland explains its chronobiotic influence on organismal activity, including the endocrine and non-endocrine rhythms. Other functions of melatonin, including its antioxidant and anti-inflammatory properties, its genomic effects, and its capacity to modulate mitochondrial homeostasis, are linked to the redox status of cells and tissues. With the aid of specific melatonin antibodies, the presence of melatonin has been detected in multiple extrapineal tissues including the brain, retina, lens, cochlea, Harderian gland, airway epithelium, skin, gastrointestinal tract, liver, kidney, thyroid, pancreas, thymus, spleen, immune system cells, carotid body, reproductive tract, and endothelial cells. In most of these tissues, the melatonin-synthesizing enzymes have been identified. Melatonin is present in essentially all biological fluids including cerebrospinal fluid, saliva, bile, synovial fluid, amniotic fluid, and breast milk. In several of these fluids, melatonin concentrations exceed those in the blood. The importance of the continual availability of melatonin at the cellular level is important for its physiological regulation of cell homeostasis, and may be relevant to its therapeutic applications. Because of this, it is essential to compile information related to its peripheral production and regulation of this ubiquitously acting indoleamine. Thus, this review emphasizes the presence of melatonin in extrapineal organs, tissues, and fluids of mammals including humans. 相似文献
94.
Gerardo?Carranza Raquel?Casta?o Mónica?L.?Fanarraga Juan?Carlos?Villegas Jo?o?Gon?alves Jesus?Avila Marco?Marenchino Ramón?Campos-Olivas Guillermo?Montoya Juan?Carlos?ZabalaEmail author 《Cellular and molecular life sciences : CMLS》2013,70(2):357-371
Tubulin cofactors (TBCs) participate in the folding, dimerization, and dissociation pathways of the tubulin dimer. Among them, TBCB and TBCE are two CAP-Gly domain-containing proteins that together efficiently interact with and dissociate the tubulin dimer. In the study reported here we showed that TBCB localizes at spindle and midzone microtubules during mitosis. Furthermore, the motif DEI/M-COO? present in TBCB, which is similar to the EEY/F-COO? element characteristic of EB proteins, CLIP-170, and α-tubulin, is required for TBCE–TBCB heterodimer formation and thus for tubulin dimer dissociation. This motif is responsible for TBCB autoinhibition, and our analysis suggests that TBCB is a monomer in solution. Mutants of TBCB lacking this motif are derepressed and induce microtubule depolymerization through an interaction with EB1 associated with microtubule tips. TBCB is also able to bind to the chaperonin complex CCT containing α-tubulin, suggesting that it could escort tubulin to facilitate its folding and dimerization, recycling or degradation. 相似文献
95.
Nocentini S Reginensi D Garcia S Carulla P Moreno-Flores MT Wandosell F Trepat X Bribian A del Río JA 《Cellular and molecular life sciences : CMLS》2012,69(10):1689-1703
Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing
cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases.
However, these cells do not present a uniform population, but instead a functionally heterogeneous population that exhibits
a variety of responses including adhesion, repulsion, and crossover during cell–cell and cell–matrix interactions. Some studies
report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients.
Here, we demonstrated that rodent OECs express all the components of the Nogo receptor complex and that their migration is
blocked by myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties
over myelin. Our data relate the decrease of traction force of OEC with lower migratory capacity over myelin, which correlates
with changes in the F-actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity
is enhanced after cell incubation with the Nogo receptor inhibitor NEP1-40. 相似文献
96.
Fourteen freshwater ostracod species collected from 24 springs in Nevada, Idaho, and Oregon between 1991 and 1994 were classified. Their ecology, based on major water parameters (i.e., temperature, dissolved oxygen, pH, conductivity), and biogeographical distribution in North America were studied. Among these 14 species, 9 ( Ilyocypris bradyi , I. gibba , Darwinula stevensoni , Candona candida , Heterocypris incongruens , Herpetocypris reptans , H. chevreuxi , Prionocypris canadensis , P. longiforma ) are new records for Nevadas ostracod fauna, while 3 ( H. reptans , P. canadensis , and Cypria turneri ) are new species for Oregon. H. reptans , P. canadensis , and Heterocypris salina are also recorded as new for the ostracod literature of Idaho. Scottia pseudobrowniana , collected from Nevada, is a new ostracod species for the United States. The occurrence of 5 Holarctic species in the northern Great Basin area may suggest a possible historical relationship with the European ostracod fauna. 相似文献
97.
Bass AJ Lawrence MS Brace LE Ramos AH Drier Y Cibulskis K Sougnez C Voet D Saksena G Sivachenko A Jing R Parkin M Pugh T Verhaak RG Stransky N Boutin AT Barretina J Solit DB Vakiani E Shao W Mishina Y Warmuth M Jimenez J Chiang DY Signoretti S Kaelin WG Spardy N Hahn WC Hoshida Y Ogino S Depinho RA Chin L Garraway LA Fuchs CS Baselga J Tabernero J Gabriel S Lander ES Getz G Meyerson M 《Nature genetics》2011,43(10):964-968
Prior studies have identified recurrent oncogenic mutations in colorectal adenocarcinoma and have surveyed exons of protein-coding genes for mutations in 11 affected individuals. Here we report whole-genome sequencing from nine individuals with colorectal cancer, including primary colorectal tumors and matched adjacent non-tumor tissues, at an average of 30.7× and 31.9× coverage, respectively. We identify an average of 75 somatic rearrangements per tumor, including complex networks of translocations between pairs of chromosomes. Eleven rearrangements encode predicted in-frame fusion proteins, including a fusion of VTI1A and TCF7L2 found in 3 out of 97 colorectal cancers. Although TCF7L2 encodes TCF4, which cooperates with β-catenin in colorectal carcinogenesis, the fusion lacks the TCF4 β-catenin-binding domain. We found a colorectal carcinoma cell line harboring the fusion gene to be dependent on VTI1A-TCF7L2 for anchorage-independent growth using RNA interference-mediated knockdown. This study shows previously unidentified levels of genomic rearrangements in colorectal carcinoma that can lead to essential gene fusions and other oncogenic events. 相似文献
98.
Martínez-Salgado C Rodríguez-Peña AB López-Novoa JM 《Cellular and molecular life sciences : CMLS》2008,65(3):477-492
The mechanisms involved in the development of renal fibrosis are poorly understood. Small Ras GTPases control cell proliferation,
differentiation, cellular growth and apoptosis, with cell-specific expression in the kidney. Cytokines, high glucose medium
or advanced glycation end-products activate Ras in different renal cells. Increased Ras activation has been found in experimental
tubulointerstitial fibrosis. Transforming growth factor-β1 (TGF-β1) and Ras signalling pathways are close related: TGF-β1
overcomes Ras mitogenic effects, and Ras counteracts TGF-β signalling. However, Ras activation is also an intracellular signal
transduction point for several molecules (e.g. TGF-β1) involved in kidney damage. Ras isoforms play different roles in regulating extracellular matrix synthesis in fibroblasts
and mesangial cells. These data give evidence for a role for Ras in renal fibrosis, but no reviews are available on the role
of p21 Ras in this process. Thus, our goal is to review the role of Ras activation and signalling in renal fibrosis.
Received 7 June 2007; received after revision 17 September 2007; accepted 1 October 2007 相似文献
99.
Carballar-Lejarazú R Rodríguez MH de la Cruz Hernández-Hernández F Ramos-Castañeda J Possani LD Zurita-Ortega M Reynaud-Garza E Hernández-Rivas R Loukeris T Lycett G Lanz-Mendoza H 《Cellular and molecular life sciences : CMLS》2008,65(19):3081-3092
Scorpine is an antimicrobial peptide whose structure resembles a hybrid between a defensin and a cecropin. It exhibits antibacterial
activity and inhibits the sporogonic development of parasites responsible for murine malaria. In this communication we report
the production of scorpine in a heterelogous system, using a specific vector containing its cloned gene. The recombinantly
expressed scorpine (RScp) in Anopheles gambie cells showed antibacterial activity against Bacillus subtilis and Klebsiella pneumoniae, at 5 and 10 μM, respectively. It also produced 98% mortality in sexual stages of Plasmodium berghei at 15 μM and 100% reduction in Plasmodium falciparum parasitemia at 5 μM. RScp also inhibited virus dengue-2 replication in C6/36 mosquito cells. In addition, we generated viable
and fertile transgenic Drosophila that overexpresses and correctly secretes RScp into the insect hemolymph, suggesting that the generation of transgenic mosquitoes
resistant to different pathogens may be viable.
Received 6 May 2008; received after revision 24 July 2008; accepted 29 July 2008 相似文献
100.
Detecting and Predicting Economic Accelerations,Recessions, and Normal Growth Periods in Real‐Time 下载免费PDF全文
Christian R. Proaño 《Journal of forecasting》2017,36(1):26-42
The dichotomous characterization of the business cycle in recessions and expansions has been central in the literature over the last 50 years. However, there are various reasons to question the adequacy of this dichotomous, recession/expansion approach for our understanding of the business cycle dynamics, as well as for the prediction of future business cycle developments. In this context, the contribution of this paper to the literature is twofold. First, since a positive rate of growth at the level of economic activity can be considered as the normal scenario in modern economies due to both population and technological growth, it proposes a new non‐parametric algorithm for the detection and dating of economic acceleration periods, trend or normal growth periods, and economic recessions. Second, it uses an ordered probit framework for the estimation and forecasting of these three business cycle phases, applying an automatized model selection approach using monthly macroeconomic and financial data on the German economy. The empirical results show that this approach has superior out‐of‐sample properties under real‐time conditions compared to alternative probit models specified individually for the prediction of recessions and/or economic accelerations. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献