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51.
Masamitsu Tsukamoto Hiroshi Kikugawa Souichiro Nagayama Taketo Suganuma Tatsuya Okita Hidenori Miyamoto 《Journal of Pesticide Science》2021,46(2):152
Tolpyralate, a new selective postemergence herbicide developed for the weed control in corn, possesses a unique chemical structure with a 1-alkoxyethyl methyl carbonate group on the N-ethyl pyrazole moiety. This compound shows high herbicidal activity against many weed species, including glyphosate-resistant Amaranthus tuberculatus. Tolpyralate targets 4-hydroxyphenylpyruvate dioxygenase (4-HPPD), which is involved in the tyrosine degradation pathway. Inhibition of the enzyme destroys the chlorophyll, thereby killing the susceptible weeds. Details of tolpyralate discovery, structure optimization, and biological activities are described. 相似文献
52.
Sakae Toyoda Hidenori Mutobe Naohiro Yoshida Yasunori Tanji 《Soil biology & biochemistry》2005,37(8):1535-1545
Isotopomer ratios of N2O, which include intramolecular 15N-site preference in addition to conventional isotope ratios for N and O in NNO (we designate Nα and Nβ for the center and end N atom, respectively, in the asymmetric molecule), reflect production and consumption processes of this greenhouse gas. Therefore, they are useful parameters for deducing global N2O budget. This paper reports the first precise measurement of 15N-site preference in N2O produced by two species of denitrifying bacteria, Pseudomonas fluorescens (ATCC 13525) and Paracoccus denitrificans (ATCC 17741).Cultures were incubated in a batch mode with a liquid medium that contains KNO3 as unique nitrogen supply under acetylene/helium (10% v/v) atmosphere at 27 °C. Enrichment factors for 15N in bulk nitrogen in N2O (average for Nα and Nβ) fluctuated in a few tens permil showing a slight difference between the species. In contrast, 15N-site preference (difference in isotope ratios between Nα and Nβ) showed nearly constant and distinct value for the two species (23.3±4.2 and −5.1±1.8‰ for P. fluorescens and P. denitrificans, respectively). The site preference was also measured for N2O produced by inorganic reactions (nitrite reduction and hydroxylamine oxidation); a unique value (about 30‰ for the both reactions) was obtained. These results and those recently reported for nitrifying bacteria suggest that 15N-site preference in N2O can be used to identify the production processes of N2O on the level of bacterial species or enzymes involved. 相似文献
53.
Kawai T Kawakita H Sugita K Saito K Tamada M Sugo T Kawamoto H 《Journal of agricultural and food chemistry》2002,50(5):1073-1076
This paper describes a cycloisomaltooligosaccharide glucanotransferase (CITase)-multilayer-immobilized porous hollow-fiber membrane used as an enzyme bioreactor. Dextran, a substrate with an average molecular mass of 43000, is converted into seven- to nine-glucose-membered cycloisomaltooligosaccharides, effective as a preventive for dental caries, aided by convective transport of the substrate to the vicinity of the enzyme through the pores. Epoxy-group-containing graft chains were uniformly appended onto the surface of pores throughout a porous hollow-fiber membrane by radiation-induced graft polymerization. Subsequently, a diethylamino group was introduced, as an anion-exchange moiety, to the graft chains, which caused the chains to expand toward the interior of the pores due to mutual electrostatic repulsion. The expanding graft chain provided multilayer binding sites for CITase. Fifty-five milligrams of adsorbed CITase per gram of membrane is equivalent to the degree of multilayer binding of 5. Finally, 80% of the multilayer-adsorbed CITase was immobilized via enzymatic cross-linking with transglutaminase to prevent the leakage of enzymes. CITase, with a degree of multilayer immobilization of 4, produced the target cycloisomaltooligosaccharides at a conversion yield of 55% in weight at 310 K during permeation by the dextran solution at a space velocity defined as the permeation rate divided by membrane volume of 6 per hour. 相似文献
54.
Macrophage activation in Theileria sergenti-infected calves was studied by testing the production of oxygen metabolites in macrophages following specific and non-specific stimulation with T sergenti merozoites or zymosan, respectively. Six calves were inoculated with merozoites and three calves with sporozoites. All showed significant macrophage activation within one month after inoculation (P less than 0.05). Activation of macrophages appeared earlier than parasitaemia or the peak of antibody titre against T sergenti. The highest chemiluminescence response, indicative of macrophage activation, was observed when the merozoites were opsonised with immune sera. 相似文献
55.
Yokoo Y Miyata H Udayanga KG Qi WM Takahara E Mantani Y Yokoyama T Kawano J Hoshi N Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1043-1050
To clarify the regulatory mechanism by bactericidal peptides secretion, the secretion of bactericidal peptides was immunohistochemically and histoplanimetrically compared with the degree of Gram-positive/negative bacterial colonization throughout the rat alimentary tract. In the associated exocrine glands from the oral cavity to the stomach, no comparable differences were observed under the changes of development of indigenous bacterial colonies. In the small intestine, immunopositive granules for lysozyme and secretory phospholipase A2 (sPLA2) were markedly decreased, whereas immunopositive vacuoles in the Paneth cells were more increased at sites with hyper-development of indigenous bacterial colonies in the intervillous spaces than at sites with no or less development. No changes in exocrine glands were observed in the large intestine because of the constant existence of large quantities of bacteria. Gram-positive bacterial colonies on the mucosal surfaces were dominant from the oral cavity to the stomach. Gram-negative bacteria were dominant in the large intestine, and the distributions of both Gram-positive and negative bacteria were intermediate in the small intestine. These findings suggest that lysozyme and sPLA2 secreted from the Paneth cells contribute to the regulation of the proliferation of indigenous bacteria in the intervillous spaces of the small intestine, and that the inversion of distributions of Gram-positive and -negative bacteria in the alimentary tract might be caused by the secretion of lysozyme and sPLA2 in the small intestine. 相似文献
56.
Supamard Panichsakpatana Hidenori Wada Makoto Kimura Yasuo Takai 《Soil Science and Plant Nutrition》2013,59(2):165-171
Several important features of the N. fixation in paddy fields which were reported previously were confirmed and some new additional results regarding the evaluation of the N2 fixation in the rhizosphere were obtained by reinvestigation in the fields. In addition, rice plants were cultivated in the submerged soil in pots and various parts of the soil were analyzed for the N2-fixing activity as well as several other properties. The results of the pot experiments were found to be fairly similar to those observed in the field investigations, indicating the validity of the submerged soil in a pot as a rather simulated model for the actual paddy field. By using this model system, the following facts were ascertained: (1) Water-percolation had almost no effect on the N2-fixing activities of both the rhizosphere and the non-rhizosphere soils. (2) Suppressing effect of washing the root of rice plant on the N2-fixing activity was slight in the seedling stage and marked in the tillering and flowering stages. (3) The N2-fixing activity of a single rice root varied from tip to base. 相似文献
57.
Abstract Extract We succeeded in isolating a γ-HCH (γ-1,2,3,4,5,6-hexachlorocyclohexane)-decomposing aerobic bacterium from an upland field where γ-HCH had been repeatedly applied for more than 10 years. The bacterium was identified as Pseudomonas paucimobilis (Oyaizu and Komagata, 1983; Senoo and Wada 1989; Wada et al. 1989). We assumed that P. paucimobilis was able to thrive in the upland field by acquiring the γ-HCH-decomposing ability (Tu 1975, 1976) based on the following mechanism: 相似文献
58.
For sustainable development of irrigated agriculture in arid regions, improvement of water use efficiency is essentially required to maintain current production levels and meet food and fiber for population growth in future. To achieve high water use efficiency, a key consideration is to reduce unnecessary soil water loss due to evaporation. In this article, regional daily evaporation over Hetao Irrigation District in a typical arid region during the irrigation period of 2009 was determined by a developed maximum surface temperature model combining Moderate Resolution Imaging Spectroradiometer (MODIS) satellite imagery. The results showed that maximum land surface temperature (LST) from MODIS satellite imagery was relatively higher in the western and middle parts than that of the eastern part of the district. At the same time, the mean minimum LST was shown somewhat higher in the eastern part. Mean daily evaporation was relatively higher in the eastern part, which showed water consuming is higher in the eastern part of the district. During the irrigation period of 2009, the total income water (irrigation water and rainfall) amount is 590.3 mm, and the outcome water (drainage discharge and evaporation) amount is 497.5 mm. The surplus of 92.8 mm in the irrigated season is considered to be consumed in winter season. Throughout the irrigated season, income and outcome almost equals each other. The daily evaporation distribution map could specify particular water consuming areas over the district where high daily evaporation may be occurred. 相似文献
59.
The influence of the application rates of cellulase preparation and glucose on silage fermentation at different temperatures was studied with the straw of naked barley (Hordeum vulgare L. emand Lam) and guineagrass (Panicum maximum Jacq.). Addition rate of cellulase and glucose, temperature and their interaction had significant effects on pH value, lactic acid content, butyric acid content and propionic acid content of naked barley straw silage and significant effects on all the parameters of guineagrass silage (P < 0.01). Temperature and interaction had significant effect on acetic acid content (P < 0.05) and no significant effect on NH3‐N content of naked barley straw silage (P > 0.05). Under all the temperatures, the pH values of barley straw and guineagrass silages were reduced by cellulase and glucose addition even at the lowest rate (P < 0.05), compared with their corresponding control. Lactic acid contents of silages were the highest within the same temperature and same additive when glucose and cellulase were added at the highest rates, whereas the effect of cellulase and glucose addition on butyric acid production varied with their application rates and silage storage temperature. The addition rate of restricting butyric acid fermentation was lower at 20°C than that at 30°C, and it was the lowest at 40°C where cellulase and glucose addition restricted butyric acid fermentation even at 0.1 g/kg and 10 g/kg, respectively, when compared to the control. While the addition rate was lower than the above level, cellulase and glucose addition also promoted butyric acid fermentation. 相似文献
60.
The chemical structures of hemicellulose and lignin are different for two distinct types of wood, i.e., softwood and hardwood.
Such differences are expected to affect pyrolysis behavior. In this article, the differences are discussed for Japanese cedar
wood (a softwood) and Japanese beech wood (a hardwood) pyrolyzed in a closed ampoule reactor (N2/600°C/40–600 s). Oven-dried samples were used to eliminate the influence of initial water. Demineralized samples (prepared
by acid washing) were also used to determine the influence of the minerals contained in the wood samples. As a result, some
features were disclosed for secondary char (coke) formation, char reactivity, tar formation, and subsequent decomposition. 相似文献