树龄对“绿宝”苹果生长及光合特性的影响

以2年生(未结果树)和5年生(结果树)的"绿宝"苹果树为试材,对生长指标(新梢生长量,干径增长量,叶面积)、光合指标(净光合速率(Pn),蒸腾速率(Tr),气孔导度(Gs),胞间二氧化碳浓度(int-CO2))及色素含量(叶绿素、类胡萝卜素)进行了测定分析。结果表明:结果树的新梢生长量、叶面积、Pn、色素含量显著或极显著高于未结果树;蒸腾速率显著低于未结果树;干径增长量、Gs、int-CO2均与未结果树无显著差异;结果树的CO2补偿点和饱和点均高于未结果树。综合分析认为,"绿宝"苹果未结果树的光合能力以及生长量较结果树低,可进一步研究采取相应栽培措施提高未结果树的同化能力。     

Effects of Lactic Acid Bacteria and Fructooligosaccharides on Growth Performance,Nutrient Digestibility and Serum Biochemical Indices of Arctic Foxes (Alopex lagopus) during Early Growth Period北大核心CSCD

This experiment was conducted to study the effects of dietary lactic acid bacteria (LAB) and fructooligosaccharides (FOS) on growth performance, nutrient digestibility and serum biochemical indices of Arctic foxes (Alopex lagopus) during early growth period. Sixty male post-litter Arctic foxes with the average body weight of (1.16±0.15) kg and at the age of (45±3) days were randomly assigned to 4 groups with 3 replicates per group and 5 foxes per replicate. The foxes were fed the following diets, respectively: basal diet (control group), basal diet+3×109 CFU / kg LAB (LAB group), basal diet+5 g / kg FOS (FOS group) and basal diet+3×109 CFU / kg LAB+5 g / kg FOS (LAB+FOS group). The experiment lasted for 30 days. The results showed as follows: 1) on the days 1 to 15, the diarrhea rate of foxes in the LAB group and LAB+FOS group was significantly lower than that in the control group and FOS group (P<0.05). On the days 16 to 30, the average daily gain of foxes in the LAB+FOS group was significantly higher than that in the control group and FOS group (P<0.05), but had no significant difference with LAB group (P>0.05); the diarrhea rate of foxes in the LAB+FOS group was significantly lower than that in the control group and FOS group (P<0.05), but had no significant difference with LAB group (P>0.05); compared with the FOS group, the feed to gain ratio of foxes in the LAB+FOS group was significantly decreased (P<0.05). 2) The dry matter digestibility of foxes in the LAB + FOS group was significantly higher than that in the control group and FOS group (P < 0.05), but had no significant difference with LAB group (P>0.05). There were no significant differences in nitrogen intake, fecal nitrogen, urine nitrogen, nitrogen deposition, net protein utilization and protein biological value among all groups (P>0.05). 3) Serum total protein (TP) content of foxes in the LAB+FOS group was significantly higher than that in the FOS group (P<0.05), but had no significant difference with control group and LAB group (P>0.05). Serum albumin (ALB) content of foxes in the LAB group was significantly higher than that in the control group and FOS group (P<0.05), but had no significant difference with LAB+ FOS group (P>0.05). Serum urea nitrogen (UN) content of foxes in the LAB+FOS group was significantly decreased compared with the control group (P < 0. 05). In conclusion, the combined supplementation of 3 × 109 CFU / kg LAB and 5 g / kg FOS in the diet is beneficial to reduce diarrhea rate, improve dry matter digestibility, improve body immune level, and promote the growth of Arctic foxes during early growth period. © 2023 Chinese Journal of Urology. All rights reserved.     

智慧农业 让山西农民挣更多的钱

<正>曾经全靠人工的农活儿,在机械、新技术的助力下,逐渐变得轻松。如今,智慧农业已不再是梦想。机械做记录农产品插上翅膀西红柿不是长在土里,而是在水里培养;稻田里,植保无人机在空中盘旋喷药;温室大棚的光线能自动调整,果蔬需要快速生长时光线直射,需要慢生长时则可斜射;扫二维码就能查到农产品的种子来源、施肥、用药情况……以上这一切不是未来,是山西已经实现了的智     

家蚕核型多角体病毒ie0基因对病毒复制和转录的影响

ie0基因是家蚕核型多角体病毒(BmNPV)的一个晚期基因,该基因编码氨基酸序列的高度保守性及多种结构域提示其在病毒感染过程中的重要性。利用Red同源重组技术,分别对BmNPV ie0基因编码不同结构域的序列以及完整序列进行敲除构建ie0缺失型病毒,以野生型病毒为对照,研究ie0序列完全或部分缺失对病毒转录、复制及对宿主细胞致病能力等方面的影响。结果表明ie0基因完整序列或编码各结构域的序列缺失后均会对病毒产生不同影响:宿主细胞中完全缺失型和部分缺失型病毒滴度均有不同程度降低;完全缺失型和部分缺失型病毒基因组的拷贝数均与野生型病毒无显著差异;部分缺失型病毒的晚期和极晚期基因转录水平变化与ie0基因完全缺失型病毒相一致,均显著低于野生型病毒,并且缺失锌指结构域编码序列的影响最为明显。MTT检测结果也显示ie0基因完全缺失后病毒对宿主细胞的致病能力有所下降。亚细胞定位显示IE0蛋白在细胞质、细胞核中均有分布,但主要位于细胞质。综上所述,ie0基因为BmNPV病毒增殖、基因组复制的非必需基因,但对病毒晚期和极晚期基因的转录具有调控作用,影响最为显著的为锌指结构域的编码序列,且该基因的缺失会导致病毒对宿主细胞的致病能力有所下降。     

偃松播种育苗技术

偃松松籽具有很高的经济价值和广阔的市场前景,是林区企业、职工和群众创收致富的重要经济来源之一。由于多年的掠夺式采摘及森林火灾,资源破坏严重,数量逐年减少,自然条件下偃松球果结实率低、质量差,特别是偃松种子天然散布能力弱,仅靠自然更新根本无法恢复到原来的偃松林状态,培育优质偃松苗是有效恢复偃松林的关键。     

1例肉鸡肝恶性黑色素瘤的病理学诊断

本研究旨在对临床上发现的1例肉鸡肝黑色素瘤进行病理学诊断。1羽25日龄AA肉鸡精神沉郁、极度消瘦,剖检发现其整个肝呈黑色。HE染色结果显示,病变肝内遍布大小不一、细胞核深染、细胞质内有大量黑色素颗粒沉积的异常细胞团。黑色素瘤诊断标志蛋白SOX 10和HMB-45抗体免疫组化染色均呈阳性。结合病理学检测和免疫组化结果,判定该病鸡肝患有恶性黑色素瘤。试验对病变肝进行胆色素染色和普鲁士蓝染色,结果均呈阴性,提示病变肝并非由胆红素或含铁血黄素淤积所致。综上表明,此例病鸡患有严重的肝恶性黑色素瘤,试验结果对禽类黑色素瘤的诊断与研究有参考价值。     

发展种养结合生态农业模式的可行性分析

种养结合生态农业模式巧妙结合种植业和养殖业,实现资源的高效利用,促进可持续发展的同时保护了生态多样性,为建设生态循环农业和乡村振兴提供了良好的发展模板.作者针对种养结合生态模式进行可行性分析,认为发展该模式行之有效:一是与现代化农业对比分析,证明其极具生态价值;二是从我国农业的基本国情以及欧美国家的种养结合情况分析,得到中国迫切需要发展种养结合生态农业模式并且具有良好的发展条件;三是从统计数据分析,种养结合生态模式良好的综合效益,先进的生态理念和时代赋予的优势条件可以为该模式的发展创造无限可能.     

三种猪用动物福利玩具在生产中的试验研究

动物福利问题日益受到人们的重视,在欧洲已经有了针对动物福利的立法。某些国家可能会把畜产品贸易与动物福利挂钩,把动物福利作为一种贸易技术壁垒限制他国的畜产品出口。动物福利将是我国的畜禽等相关产品走向健康化、走向国际市场,必须遵守的国际规则,不断改善畜禽的饲养方式和生存环境,善待畜禽,保证畜禽基本的生存福利,才能保证动物的健康,保证动物性产品的安全,才能促进我国畜牧业的健康持续发展。