L-肉毒碱对阿霉素性心肌损伤保护作用的研究——心肌线粒体膜流动性测定

阿霉素可导致剂量累积性心肌细胞损伤。L-肉毒碱已确认能减轻心肌细胞形态学改变。本实验测定了心肌细胞线粒体膜流动性,目的是从生物化学角度观察L-肉毒碱对阿霉素性心肌损伤的作用。实验结果表明:阿霉素可引起线粒体膜流动性的明显改变。而补充外源肉毒碱可改善这种状态。     

一次性引流袋的妙用

从胃管内注入豆油是临床上常用的治疗肠梗阻的护理操作技术之一.传统方法是用50 mL注射器推注.推注的缺点是:如推注力量稍小,则因油脂黏稠使推注操作困难;用力过大,则使病人出现恶心,甚至呕吐.针对上述情况,我科自2004年1月至今,采用改进后的一次性引流袋代替注射器用于从胃管内注入豆油,临床观察180例肠梗阻病人,效果满意.现介绍如下.……     

粒细胞集落刺激因子治疗大鼠局灶性脑缺血再灌注损伤

OBJECTIVE: To study the therapeutic effects of granulocyte colony-stimulating factor (G-CSF) on focal cerebral ischemia-reperfusion injury in rats. METHODS: Adult male SD rats were subjected to transient (2 h) middle cerebral artery occlusion (MCAO) and scored 48 h later for such neurological functions as spontaneous movement, paresis, forelimb motor function, climbing ability, pain sensation, and position sense. Twenty rats with the total score less than 12 were divided equally into two groups to receive daily subcutaneous injection of 20 microg/kg of G-CSF for 19 days or the same dose of saline injection (control group). The rats in the two groups were also given intraperitoneal injection of Brdu10 mg/kg for 19 days. The neurological functions of the rats in both groups were examined on a weekly basis after MCAO and on day 21, the rats were killed to prepare frozen sections of the brain tissues for double immunohistochemical staining with Brdu and glial fibrillary acidic protein antibody to identify the neural cells newly evolved from the stem cells. RESULTS: The rats in G-CSF group showed better neurological function recovery than the control rats 3 weeks after MCAO (P<0.05). Obvious regeneration of the neural cells was observed around the infarction area in the rats receiving G-CSF treatment. CONCLUSION: G-CSF promotes neurological function recovery and neural cell regeneration after cerebral infarction in rats and can be effective for intervention of focal cerebral ischemia-reperfusion injury.     

胰腺癌细胞的增殖同膜脂质饱和度,膜胰岛素受体的关系

外源性表皮生长因子(或生长抑素)能促进(或抑制)胰腺癌细胞的增殖。胰腺癌细胞增殖速率的高低与细胞膜脂质饱和度的降、升;与膜胰岛素受体数量的增减密切相关。测定细胞膜脂质饱和度和胰岛素受体数量,是判断胰腺癌增殖状态的灵敏指标,有一定临床价值。     

Subclinical hepatic encephalopathy: relationship between neuropsychological deficits and standard laboratory tests assessing hepatic status.

A multivariate analysis of the relationship between biochemical measures of hepatic function and neuropsychological assessments of specific cognitive domains was performed on data obtained from 74 patients with chronic liver disease and subclinical hepatic encephalopathy. Biochemical tests of hepatic protein synthesis correlated significantly with measures of impaired language efficiency, perceptual speed, and psychomotor efficiency. Biochemical indices of impaired processing of nitrogenous compounds correlated with visuopractic deficits. Hepatic blood flow indices correlated with language inefficiency. Interestingly, biochemical measures of hepatic injury did not demonstrate a significant association with any neuropsychological parameter assessed. These results suggest that subclinical hepatic encephalopathy is the consequence of a multifactorial hepatic dysfunction, and that acute hepatic injury, as assessed by elevation of aminotransferases, does not appear to be involved in the pathogenesis of subclinical hepatic encephalopathy.     

传染病研究40年（1955～1995年）

本文综述本校传染病学教研室１９５５～１９９５年间所取得的科研成果。内容包括１０余种传染病与寄生虫病的临床和基础研究，其中以华支睾吸虫病、恙虫病等有广东特色的传染病以及伤寒、痢疾、病毒性肝炎等影响人民健康最普遍的传染病为重点。反映了建国以来各个时期本教研室对防治这些传染病所作的贡献。所取得的成果，相当一部分通过多年来的验证，已获得广大传染病工作者所认同或列为常规，部分已获得部委级奖励。现在重温这些成果，可能起承前启后的意义。     

维生素E琥珀酸酯诱导人胃腺癌细胞凋亡途径

目的　探讨维生素E琥珀酸酯 (VES)诱导人胃腺癌SGC -790 1细胞凋亡的死亡受体 (Fas)信号转导途径。方法　人胃腺癌SGC -790 1细胞经不同剂量VES(5,10 ,2 0 μg/ml)处理 ,同时做琥珀酸、维生素E和空白对照 ,采用DAPI(4,6-贰脒基 -2 -苯基吲哚 )荧光染色法观察细胞凋亡情况 ,用WesternBlot法检测Fas、带有死亡结构域的Fas相关蛋白 (FADD)和天冬氨酸特异性半胱氨酸蛋白酶 (caspase -8)蛋白表达情况 ,Fas和FADD反义寡聚核苷分别转染SGC -790 1细胞后 ,用荧光法检测caspase -8活性。 结果　经VES处理后的细胞DAPI染色可见凋亡的形态学改变 ,2 0 μg/mlVES处理 48h后的细胞凋亡率为 89.6% ;VES处理 48h后Fas、FADD和caspase -8蛋白表达明显增加 ,且呈剂量 -效应关系 ;阻断Fas可明显抑制FADD蛋白表达 ,Fas和FADD反义寡聚核苷转染细胞后caspase -8活性明显降低 (P <0 .0 1) ,其中阻断Fas的效果高于阻断FADD。结论　维生素E琥珀酸酯诱导人胃腺癌SGC -790 1细胞凋亡过程中启动了Fas信号转导途径 ,VES启动Fas后 ,FADD将Fas和caspase -8联接起来 ,活化caspases级联反应 ,从而构成Fas/FADD/caspase -8的凋亡信号途径     

低强度超短波对小鼠体质及智力的影响

本实验探讨了低强度超短波对小鼠连续三代辐射,对学习、记忆、拉力、运动耐力和周围血象的影响。用81MHz,10～12V/m 的超短波进行辐射,每天辐射2小时,每周6天。结果表明:经过超短波辐射的各代小鼠与对照组相比,学习能力、记忆能力、拉力及运动耐力有明显的降低,且运动耐力有进行性降低的趋势,而经过辐射的三代小鼠红细胞、血红蛋白含量及白细胞数均明显增高,每个红细胞的血红蛋白含量则低于对照组。     

MRI诊断儿童脑白质病变的初步探讨

目的 :初步探讨 MRI诊断儿童脑白质病变的作用和特异征象。方法 :对 MRI诊断为脑白质病变的病例 4 8例进行观察 ,分析病灶的 MRI征象 ,包括病灶出现的部位、形态、范围及其他合并表现。结果 :1病灶发生的部位 ,多位于双侧侧脑室三角部周围的白质内 ,依次为侧脑室前角周围、半卵圆中心 ,偶见于基底节区等。2形态 :多为小斑片状 ,亦有点线状、小点状。 3范围 :以侧脑室三角部周围白质内发生多见 ,常同时出现于多个部位。 4时常伴有其他合并征象 ,如 ,胼胝体发育不良、畸形或脑室扩大等。结论 :MRI扩展了对儿童脑白质病变的认识 ,是一种较好的手段     

软组织复发及多发性平滑肌肉瘤的临床病理、免疫组织化学及电镜研究

本组报告软组织复发及多发性平滑肌肉瘤22例(多发性2例),进行了组织学、免疫组织化学及电镜观察。本组20例复发性平滑肌肉瘤复发次数为1～5次,间隔时间为2月～12年,病理可分为高分化型、低分化型、中度分化型及临界型平滑肌肉瘤。临界型易被忽略,应进行随访。