Determination of Optimum Burn-In Time: A Composite Criterion

The object of this paper is to present a mathematical model capable of determining the optimum amount of time that semiconductor devices, which have specified life characteristics, must be placed on burn-in to obtain maximum performance versus total cost. To make the model operational and realistic, the traditional assumption of an exponential (more recently, Weibull) distribution of life is omitted in favor of the generalized gamma distribution (GGD). This is done because the GGD includes, as special cases, such distributions as the normal, Rayleigh, Maxwell, chi, chi2, Weibull, exponential, ordinary gamma, etc. The use of the greater representational capability of the GGD is justified in the results of the studies showing that (other things being equal) small changes in parametric values of life characteristics can cause vast differences in the optimum burn-in time and maximum system effectiveness. The physical performance sector of the model incorporates system effectiveness that includes such factors as availability, expected time to repair, mission reliability, system use coefficient, storage survival probability, and operational readiness. The costs considered are those due to burn-in operation, production, and sales. The model has been studied by use of computer runs from the standpoint of critical analysis and parametric sensitivity analysis.     

Implementing Compositional Analysis Using Intersection Types With Expansion Variables

A program analysis is compositional when the analysis result for a particular program fragment is obtained solely from the results for its immediate subfragments via some composition operator. This means the subfragments can be analyzed independently in any order. Many commonly used program analysis techniques (in particular, most abstract interpretations and most uses of the Hindley/Milner type system) are not compositional and require the entire text of a program for sound and complete analysis.System is a recent type system for the pure λ-calculus with intersection types and the new technology of expansion variables. System supports compositional analysis because it has the principal typings property and an algorithm based on the new technology of β-unification has been developed that finds these principal typings. In addition, for each natural number k, typability in the rank-k restriction of System is decidable, so a complete and terminating analysis algorithm exists for the rank-k restriction.This paper presents new understanding that has been gained from working with multiple implementations of System and β-unification-based analysis algorithms. The previous literature on System presented the type system in a way that helped in proving its more important theoretical properties, but was not as easy to follow as it could be. This paper provides a presentation of many aspects of System that should be clearer as well as a discussion of important implementation issues.     

Reproducibility of quantitative proteomic analyses of complex biological mixtures by multidimensional protein identification technology

If quantitative proteomic technologies are to be of widespread use to the biological community, the reproducibility of each method must be investigated and determined. We have analyzed the reproducibility of complex quantitative proteomic analyses of metabolically labeled S. cerevisiae analyzed via multidimensional protein identification technology (MudPIT). Three independent cell growths of S. cerevisiae grown in rich and minimal media and independent MudPIT analyses of each were compared and contrasted. Quantitative MudPIT was found to be intra- and interexperimentally reproducible at both the peptide and protein levels. Proteins of potential low abundance were detected, identified, and quantified by identical peptides from three independent samples. In addition, when multiple peptides were matched to a protein, the relative abundance of each peptide was in agreement across the three samples. Despite the reproducibility, errors in the experimental determination of protein expression levels occurred, but the impact of the variation was minimized by replicate experiments. Last, quantitative MudPIT analyses will likely be improved by increasing the number of peptide hits per protein in a given analysis, which will provide for greater intraexperimental reproducibility.     

On not losing track

Tracking time is used as an explicit objective in designing tracking algorithms that function in clutter. Implications of using time to the first mistake as a measure of tracking time are explored. An optimal tracker is derived for a special circumstance where there is clutter but no measurement error, and is compared to a tracker based on the maximum likelihood principle     

Controlled manipulation of molecular samples with thenanoManipulator

The nanoManipulator system adds a virtual-reality interface to an atomic-force microscope (AFM), thus providing a tool that can be used by scientists to image and manipulate nanometer-sized molecular structures in a controlled manner. As the AFM tip scans the sample, the tip-sample interaction forces are monitored, which, in turn, can yield information about the frictional, mechanical, material, and topological properties of the sample. Computer graphics are used to reconstruct the surface for the user, with color or contours overlaid to indicate additional data sets. Moreover, a force feedback stylus, which is connected to the tip via software, allows the user to directly interact with the macromolecules. This system is being used to investigate carbon nanotubes, DNA, fibrin, adeno- and tobacco mosaic virus. It is now also possible to insert this system into a scanning electron microscope which provides the user with continuous images of the sample, even while the AFM tip is being used for manipulations     

POROSITY IV: THE USE OF PETROLEUM PRODUCTS AS ABSORPTION LIQUIDS

Advantages of petroleum products .—-Avoidance of: slaking, chemical reaction, adsorption, and solvent action; but longer soaking time is required. Paraffine and vaseline .—-Materials having high fluidity when hot, and comparatively low fluidity when cold, permit surface of saturated test piece to be brought to definite and reproducible condition with all surface pores full. Paraffine may be used but vaseline is preferable because it undergoes no change of phase on cooling. Procedure and results .—-A saturation procedure is described and comparative results given. Density and penetrativity of vaseline .—-Sp. gr.23°/4° 0.8730, 32°/4° 0.8684, 42°/4° 0.8624. Penetrativity between 100° and 200°C = 0.063 (t— 30)² cms./sec. A penetratimeter for determining the penetrativity of liquids is described     

Materials study of silicon-on-lnsulator material by TEM

Silicon-on-insulator (SOI) technology addresses the need for many different device applications, such as radiation tolerant devices, high voltage, and three-dimensional circuitry applications. Isolated silicon epitaxy (ISE) is a commercialised process which results in excellent SOI material quality with proven results, having overcome most of the obstacles of other processes, although only having reduced, not eliminated, threading dislocations. The remaining isolated dislocations have been examined in detail by transmission electron microscopy (TEM). These have been diagnosed as normal lattice dislocations, with no faults or twins in the material. The nature, source, and behavior of the remaining dislocations is discussed.     

POROSITY: V. RECOMMENDED PROCEDURES FOR DETERMINING POROSITY BY METHODS OF ABSORPTION

Detailed Procedure. —For shaped test pieces details of the procedure are given under the headings. “Preparation of Test Piece.”“Determination of Dry Weight,”“The Absorption Apparatus,”“Preparation of Standard Vaseline,”“Saturation Procedure,”“Calculation,” and “True Density.” A Pycnometer Method. —A new and accurate method, which is applicable equally to shaped test pieces or to granular material, is described. Among other advantages it gives the operator control over the max. size opening which he desires to class as part of the pore space. It also can be made to indicate directly the necessary soaking period.     

POROSITY: III. WATER AS AN ABSORPTION LIQUID

Classes of absorption method .—The ordinary immersion method has been shown by previous investigators to be unreliable. Simple immersion in low vacuo without boiling is also shown theoretically to be unreliable. An analysis of the results of previous investigators indicates the presence of unsuspected sources of error. Effect of adsorbed gases upon dry weights of test pieces .—Dry air is shown to be without appreciable influence. Depending upon the humidity of the atmosphere, adsorbed water vapor may cause errors up to 2 per cent in the porosity value. Perfectly dry fired clay will remove water from conc. H₂SO₄, and from fused CaCl₂. Saturation by boiling at atmospheric pressure .—A one hour's (and in one instance a 5 hours') boiling failed to saturate completely. On continued boiling the saturated weight increases linearly with the time and this in spite of the fact that appreciable quantities of dissolved materials are removed from the test piece by the hot water. This result is shown to be due to a gradual and continuous rehydration of the clay by the hot water. The error from this factor may amount to as much as 3% and the error from dissolved materials to as much as 2%, during a 3 hours' boiling. The nature of the dissolved material was determined. Method of cooling the test piece .—It is recommended that the test piece be kept in a closed vessel over 95% sulphuric acid for several hours before its dry weight is taken. Saturation procedure .—A vacuum method is described in which water may be employed as the saturation liquid under conditions where the above sources of error are reduced to a minimum. The method is not however recommended as a primary standard. Necessity of a soaking period .—It is shown from theoretical considerations that a soaking period is necessary and methods are given for calculating and for measuring the minimum soaking period required for a given test piece and a given liquid of known penetrativity.     

DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format

High affinity capture agents against protein targets are essential components for immunoassays, regardless of specific analysis format. Here, we describe the use of DNA-encoded antibodies for rapidly screening the kinetic and equilibrium binding properties of twelve commercial antibodies in a parallel analysis format using a multiplexed array of microring optical resonators. We show that DNA-encoding offers advantages in terms of antigen binding capacity, compared to covalently tethered antibodies; we also demonstrate that this linkage modality facilitates the rapid self-assembly of multiplexed arrays on account of complementarity between the DNA sequences on the antibodies and sensor array, respectively. Furthermore, DNA-encoded antibodies also allow for sensor array regeneration and reprogramming, as chaotropic agents can be used to disrupt the DNA-DNA duplexes that link the capture agents to the sensor without harming the underlying DNA on the surface, which can subsequently be reloaded with antibodies either targeting the same or different antigens.