Recent developments in the understanding of antinuclear autoantibodies

Cytological identification of soybean mitotic metaphase chromosomes (2n = 40) has been severely limited by their small size and uniform karyomorphology. We have developed fluorescent in situ hybridization (FISH), PCR-primed in situ labelling (PCR-PRINS) procedures, and molecular probes for routine cytological identification and for the physical mapping of soybean somatic chromosomes. Chromosome preparation has been achieved by modifications of previous protocols and through the preparation of root-tip protoplasts prior to chromosome spreading. Initially our probe selection focused on highly repeated DNAs that provide very intense localized hybridization signals. Repetitive gene probes that have proven valuable include the rDNA loci (5S and 45S) which are chromosome specific. We have also developed satellite DNA probes for two different sequence families: the SB92 and the STR120 satellites. Both of these are tandemly arranged at multiple chromosomal loci. By using different cloned examples of each family, we have been able to selectively label unique subsets of soybean chromosomes. Double hybridization with biotin and digoxigenin labeled probes has allowed us to determine the chromosomal overlap between different probes. In addition, we have joined portions of the metaphase chromosome painting patterns with the genetic map by single-copy FISH and PCR-PRINS detection of the RFLP loci G8.15, G17.3, and A199a and A199b. Total genomic DNA in situ hybridization (GISH) patterns were also used to characterize the soybean chromosomes.     

1－氨基蒽醌的开发

介绍了１－氨基蒽醌生产方法及革新工艺，对１５０ｔ／ａ精馏法生产１－氨基蒽醌的效益进行了计算。并浅谈了其重要衍生产品溴氨酸。     

Compressive and tribological properties of Al2O3 fibre and hexagonal BN particle hybrid reinforced Al-Si alloys

Al₂O₃ fibre-hexagonal BN particle hybrid reinforced aluminium-silicon alloys were fabricated by centrifugal force infiltration route. Hardness test and ultimate compressive test results are reported. The wear and friction properties of hybrid MMCs was investigated by means of a block-on-ring (bearing steel) type wear rig in a dry sliding condition. It is shown that the hardness and ultimate compressive strength of hybrid MMCs was evidently decreased with the addition of hexagonal BN particles, however, the wear rate and coefficient of friction of hybrid MMCs was improved simultaneously with increase of BN particle volume fraction, especially for the higher applied loads in the test.     

Efficient encoding of IEEE 802.11n LDPC codes

Addressed is the issue of LDPC coding for the emerging IEEE 802.11n standard. An efficient encoding algorithm is proposed. The algorithm is simple and easy to implement. The memory requirement is trivial     

利用假单胞菌天冬氨酸－β－脱羧酶高效生产L－丙氨酸

通过诱变筛选得到一株具有高活性Ｌ－Ａｓｐ－β－脱羧酶的菌株ＰｓｅｕｄｏｍｏｎａｓＮＸ－１，对该株酶形成和酶反应的条件进行了详细研究，该株可以高效地转化Ｌ－Ａｓｐ生成Ｌ－ＡＩａ，转化４～５山每Ｌ培养液可转化Ｌ－Ａｓｐ量高达１４００ｇ左右，生成Ｌ－Ａｌａ浓度高达９０％以上，摩尔转化率近１００％。     

浅除盐水技术在合成氨水处理工艺中的应用

在合成氨生产过程中，要根据不同的工序要求对用水进行净化处理，很多合成氨生产厂尤其中小合成氨厂的水处理主要采用仅除去硬度的软化水作为一般蒸气锅炉给水和工艺用水。有自备电站的成氨厂锅炉炉水一般采用一级复床除盐水系统制备除盐水（电导率≤１０μｓ／ｃｍ） 工艺软化水制造成本低但其仅除去原水中的硬度而盐分没有减少，作为锅炉给水造成锅炉排污量大热损失大作为工艺用水常引起合成系统变换催化剂的表面结垢等门题，除盐水的水质好，能完全满足合成氨生产工艺用水的需要，但其制造成本为工艺水的几倍。本文主要介绍浅除盐水技术…     

关于保障鞍山市城市饮水安全的研究及对策

本文在全面阐述鞍山市水资源现状的基础上,系统地分析了鞍山市供水系统存在的问题,并针对鞍山实际情况,提出了保障鞍山市城市饮水安全的相关研究及对策。     

酶促合成生物柴油反应动力学

以Candidasp.99-125脂肪酶为催化剂,甘油三油酸酯和甲醇为底物,采用有序机制模型对酶促合成生物柴油的酯交换反应动力学进行了研究,并与经典乒乓机制模型进行了比较。研究结果表明,反应初速率的实验值与有序机制模型方程的计算值吻合很好。对于固定化Candidasp.99-125脂肪酶催化合成生物柴油的酯交换反应机理进行研究,采用有序机制模型比经典乒乓机制模型更为精确。反应过程中,醇抑制为竞争性抑制,在甘油三油酸酯浓度较小的范围内,醇抑制作用较为显著,醇浓度越低反应初速率越快。该有序机制模型可用来预测生物柴油的生产批次或连续反应器中酯交换反应的速率,确定最佳底物油脂和醇的浓度。