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41.
42.
Hayashi KG Matsui M Shimizu T Sudo N Sato A Shirasuna K Tetsuka M Kida K Schams D Miyamoto A 《Reproduction (Cambridge, England)》2008,136(6):787-797
We previously established a bovine experimental model showing that the corpus luteum (CL) does not appear following aspiration of the preovulatory follicle before the onset of LH surge. Using this model, the present study aimed to determine the profile of follicular development and the endocrinological environment in the absence of CL with variable nadir circulating progesterone (P(4)) concentrations during the oestrous cycle in cattle. Luteolysis was induced in heifers and cows and they were assigned either to have the dominant follicle aspirated (CL-absent) or ovulation induced (CL-present). Ultrasound scanning to observe the diameter of each follicle and blood collection was performed from the day of follicular aspiration or ovulation and continued for 6 days. The CL-absent cattle maintained nadir circulating P(4) throughout the experimental period and showed a similar diameter between the largest and second largest follicle, resulting in co-dominant follicles. Oestradiol (E(2)) concentrations were greater in the CL-absent cows than in the CL-present cows at day -1, day 1 and day 2 from follicular deviation. The CL-absent cows had a higher basal concentration, area under the curve (AUC), pulse amplitude and pulse frequency of LH than the CL-present cows. After follicular deviation, the CL-absent cows showed a greater basal concentration, AUC and pulse amplitude of growth hormone (GH) than the CL-present cows. These results suggest that the absence of CL accompanying nadir circulating P(4) induces an enhancement of LH pulses, which involves the growth of the co-dominant follicles. Our results also suggest that circulating levels of P(4) and E(2) affect pulsatile GH secretion in cattle. 相似文献
43.
Natsui Shungo Sudo Takuya Shibuya Ryota Nogami Hiroshi Kikuchi Tatsuya Suzuki Ryosuke O. 《Metallurgical and Materials Transactions B》2020,51(1):11-15
Metallurgical and Materials Transactions B - An in situ observation technique of the TiO2 interfacial behavior in molten LiCl-KCl electrolysis was developed. The variation of the thin TiO2... 相似文献
44.
This paper describes the amplification characteristics of gain-flattened Er3+-doped fiber amplifiers (EDFAs) by using 0.98-μm and 1.48-μm band pumping for a 1.58-μm band WDM signal. Silica-based Er3+-doped fiber (S-EDF) and fluoride-based Er 3+-doped fiber (F-EDF) have gain-flattened wavelength ranges from 1570 to 1600 nm and from 1565 to 1600 nm, respectively, and exhibit uniform gain characteristics with gain excursions of 0.7 and 1.0 dB, and the figure of merit of the gain flatness (gain excursion/average signal gain) of 3 and 4.3%, respectively, for an eight-channel signal in the 1.58-μm band. We show that 1.48-μm band pumping has a better quantum conversion efficiency and gain coefficient, and that 0.98-μm band pumping is effective for improving the noise characteristics. We also show that the EDFAs consisting of two cascaded amplification units pumped in the 0.98-μm and 1.48-μm bands are effective in constructing low-noise and high-gain 1.58-μm band amplifiers 相似文献
45.
Lo KM; Sudo Y; Chen J; Li Y; Lan Y; Kong SM; Chen L; An Q; Gillies SD 《Protein engineering, design & selection : PEDS》1998,11(6):495-500
We have developed a general expression system that enhances the production
and secretion of proteins in mammalian cells. The protein of interest is
expressed as a fusion to a signal peptide and the Fc fragment of
immunoglobulin as the N-terminal fusion partner, which can direct the
cellular processes into expressing and secreting high levels of many
different types of proteins. These include secretory proteins, enzymes and
soluble domains of membrane proteins, as well as nuclear and regulatory
proteins. Typical expression levels of these proteins from stable cell
lines ranged from several to 100 microg/ml in conditioned media. The Fc
domain helps to solubilize hydrophobic proteins and provides a handle for
easy detection and purification of the fusion proteins; and it can be
cleaved off by treatment with protease if desired.
相似文献
46.
Yukio Sudo 《亚洲传热研究》1998,27(7):509-521
The characteristics of critical heat flux (CHF) in existing experiments under high subcooling and high velocity in short heated channels have, for the first time, been systematically and quantitatively investigated to provide a CHF correlation that can properly predict the effect of channel length, especially when the channel length-to-channel diameter ratio L/D is less than about 20. The major test conditions of existing CHF experiments investigated in this study were channel diameter 1 to 4 mm, L/D 1 to 25, 0.1 to 1.2 MPa pressure, 34 to 117°C inlet water subcooling and 500 to 40 700 kg/(m2 · s) mass flux in circular channels, and 3 to 20 mm gap size, 6 to 40 L/De, 0.1 to 3.1 MPa pressure, 4 to 166°C inlet water subcooling, and 940 to 27,000 kg/(m2 · s) mass flux in rectangular channels. The effect of L/D on CHF was evaluated referring to the analytical solution of CHF, which was previously derived by the author for the channel flow at high subcooling and high velocity. As a result, the effect of L/D was quantitatively clarified as an effect of magnitude in heat transfer of single-phase forced-convection flow, giving a larger CHF with a smaller L/D in the case of L/D less than about 20. The proposed correlation predicts CHF to within a ±35 percent error margin. ©1998 Scripta Technica, Heat Trans Jpn Res, 27(7): 509–521, 1998 相似文献
47.
An experiment was performed to simulate a water cooling panel system for decay heat removal from a high-temperature gas-cooled reactor (HTGR) and to investigate the performance of decay heat removal and the temperature distribution for components of the system. The experimental apparatus is composed of a pressure vessel 1 m in diameter and 3 m in height, containing heaters with a maximum heating rate of 100 kW which simulates the decay heat of the reactor core and cooling panels surrounding the pressure vessel. The analytical code THANPACST2 was applied to analyze the experimental data and to investigate the validity of the analytical method and model proposed. Under conditions using helium gas at a pressure of 0.73 MPa and temperature of 210°C in the pressure vessel, temperatures of the pressure vessel were well estimated to within differences of −29 to +37°C compared to the experimental data. The analyses indicate that the heat removed by the cooling panel is 11.4% less than the experimental value and the heat transferred by thermal radiation is 74.4% of the total heating value. It was also found that the lower head of the pressure vessel is effectively cooled by natural convection through the flow channels at the upper and the lower edges of the skirt-type support of the pressure vessel. © 1998 Scripta Technica, Heat Trans Jpn Res, 26(3): 159–175, 1997 相似文献
48.
Yukio Sudo 《亚洲传热研究》1997,26(1):16-29
A quantitative analysis of critical heat flux (CHF) under high mass flux with high subcooling at atmospheric pressure was successfully carried out by applying a new transition region model for a macro-water sublayer on heated walls to the existing model of a vapor blanket over the macro-water sublayer. The CHF correlation proposed in this study could predict well the experimental data obtained for water mass flux of 940 to 20,300 kg/m2s using circulate tubes 2 to 4 mm in diameter and 30 to 100 mm in length with inlet subcooling of 30 to 90 °C and rectangular channels heated from one side with gaps of 3 to 20 mm, length of 50 to 305 mm, and inlet subcooling of 30 to 77 °C and revealed a unique feature of CHF, namely, that the effects of wall friction of subcooled boiling flow and the velocity of the steam blanket above the macro-water sublayer at atmospheric pressure become the dominant factors while they were not dominant at higher pressures. © 1997 Scripta Technica, Inc Heat Trans Jpn Res, 26 (1): 16–29, 1997 相似文献
49.
Yasutaka Kamei Atsushi Sudo Haruo Nishida Kiyoshi Kikukawa Takeshi Endo 《Polymer Bulletin》2008,60(5):625-634
N-(4-Nitrophenoxycarbonyl)-γ-benzyl-L-glutamate (1) was heated at 60 °C in N,N-dimethylacetoamide (DMAc) in the presence of p-(tert-butyl)phenylmethylamine (2) to afford poly(γ-benzyl-L-glutamate)
in high yield. 1H NMR analysis of the obtained polymer revealed that the
p-(tert-butyl)phenylmethyl group
was successfully introduced into the polymer chain end. Under the same conditions, polycondensations
of 1 in the presence of poly(ethylene glycol)s having amino terminals
were performed. Both of the components were consumed quantitatively to afford the corresponding block
copolymers having poly(γ-benzyl-L-glutamate) and poly(ethylene glycol) segments. The molecular weights
of the copolymers were controlled by the feed ratio [1]0/[amino
group in the macroinitiator]0.
Electronic Supplementary Material The online version of this article at
contains supplementary
material, which is available to authorized users. 相似文献
50.
Tomiko M. Suzuki Tadashi Nakamura Eiichi Sudo Yusuke Akimoto Kazuhisa Yano 《Microporous and mesoporous materials》2008,111(1-3):350-358
A new type of sulfonic acid-functionalized monodispersed mesoporous silica spheres (MMSS) were synthesized directly by co-condensation and subsequent oxidation. By changing the methanol ratio, sulfonic acid-functionalized MMSS with different particle diameters (390–830 nm) and the same mesopore sizes were successfully synthesized. TEM observations revealed that the mesopores were aligned radially from the center towards the outside of the spheres, even in the sulfonic acid-functionalized MMSS. The catalytic activities of the sulfonic acid-functionalized MMSS were studied in condensation reactions between 2-methylfuran and acetone, and it was found that their catalytic activities are highly dependent on the particle diameters. In addition, the catalytic activity of MMSS was much higher than that of other forms of mesoporous silica due to its radially-aligned mesopores. 相似文献