A 2.5-ns clock access, 250-MHz, 256-Mb SDRAM with synchronousmirror delay

A 256-Mb SDRAM (245.7 mm²) has been developed using (1) a high cell occupation ratio (60.2%) array design for chip size reduction and a high yield, (2) a prefetched pipeline scheme (PPS) using a first-in first-out (FIFO) buffer with parallel serial converter for 250-MHz clock frequency operation, and (3) a synchronous mirror delay (SMD) circuit for 2.5-ns clock access and low standby current     

Cellulose promotes extracellular assembly of Clostridium cellulovorans cellulosomes

Cellulosome synthesis by Clostridium cellulovorans was investigated by growing the cells in media containing different carbon sources. Supernatant from cells grown with cellobiose contained no cellulosomes and only the free forms of cellulosomal major subunits CbpA, P100, and P70 and the minor subunits with enzymatic activity. Supernatant from cells grown on pebble-milled cellulose and Avicel contained cellulosomes capable of degrading crystalline cellulose. Supernatants from cells grown with cellobiose, pebble-milled cellulose, and Avicel contained about the same amount of carboxymethyl cellulase activity. Although the supernatant from the medium containing cellobiose did not initially contain active cellulosomes, the addition of crystalline cellulose to the cell-free supernatant fraction converted the free major forms to cellulosomes with the ability to degrade crystalline cellulose. The binding of P100 and P70 to crystalline cellulose was dependent on their attachment to the endoglucanase-binding domains of CbpA. These data strongly indicate that crystalline cellulose promotes cellulosome assembly.     

Remittances and educational outcomes: a regional investigation for Moldova

This paper looks at the relationship between remittances and higher education in Moldovan regions. The objective is to test whether remittances are associated with an increase in the likelihood of attending higher education institutions by taking into account a possible disruptive effect of migration and looking for regional differences in this relationship. Findings show that, on average, remittances are associated with a 5.4 percentage point increase in the likelihood that young individuals will pursue higher education. Nonetheless, this result is widely heterogeneous across regions.     

Multiple Superconducting Gaps and Anisotropic Spin Fluctuations in Hole-Doped and Electron-Doped Iron-Pnictides: NMR Studies

We present extensive ⁷⁵As-NMR data on the electron-doped pnictides PrFeAsO_0.89F_0.11 (T _c=45 K), LaFeAsO_0.92F_0.08 (T _c=23 K), and the hole-doped Ba_0.72K_0.28Fe₂As₂ (T _c=31.5 K) single crystal. We find that the Fe antiferromagnetic spin fluctuations are anisotropic and are weaker compared to underdoped copper-oxides or cobalt-oxide superconductors. The spin lattice relaxation rate 1/T ₁ decreases below T _c with no coherence peak and shows a step-wise variation at low temperatures. The Knight shift decreases below T _c and shows a step-wise T variation as well. These results indicate spin-singlet superconductivity with multiple gaps.     

Cracking of n-Butane Over Alkaline Earth-Containing HZSM-5 Catalysts

A series of catalysts, NiSO₄/SiO₂–Al₂O₃, for ethylene dimerization were prepared by the impregnation method using aqueous solution of nickel sulfate. Although SiO₂–Al₂O₃ without NiSO₄ was inactive as catalyst for ethylene dimerization, the SiO₂–Al₂O₃ with NiSO₄ exhibited high catalytic activity even at room temperature. The high catalytic activity of NiSO₄/SiO₂–Al₂O₃ was closely correlated with the increase of acidity and acid strength due to the addition of NiSO₄. The sample having 15 wt% of NiSO₄ and calcined at 500 °C for 1.5 h exhibited maxima for catalytic activity and acidity. In view of IR results of CO adsorbed on NiSO₄/SiO₂–Al₂O₃, it is concluded that the active sites responsible for ethylene dimerization consist of a low-valent nickel, Ni⁺, and an acid.     

Elevated Production of Mitochondrial Reactive Oxygen Species via Hyperthermia Enhanced Cytotoxic Effect of Doxorubicin in Human Breast Cancer Cell Lines MDA-MB-453 and MCF-7

Hyperthermia (HT) treatment is a noninvasive cancer therapy, often used with radiation therapy and chemotherapy. Compared with 37 °C, 42 °C is mild heat stress for cells and produces reactive oxygen species (ROS) from mitochondria. To involve subsequent intracellular accumulation of DOX, we have previously reported that the expression of ATP-binding cassette sub-family G member 2 (ABCG2), an exporter of doxorubicin (DOX), was suppressed by a larger amount of intracellular mitochondrial ROS. We then hypothesized that the additive effect of HT and chemotherapy would be induced by the downregulation of ABCG2 expression via intracellular ROS increase. We used human breast cancer cell lines, MCF-7 and MDA-MB-453, incubated at 37 °C or 42 °C for 1 h to clarify this hypothesis. Intracellular ROS production after HT was detected via electron spin resonance (ESR), and DOX cytotoxicity was calculated. Additionally, ABCG2 expression in whole cells was analyzed using Western blotting. We confirmed that the ESR signal peak with HT became higher than that without HT, indicating that the intracellular ROS level was increased by HT. ABCG2 expression was downregulated by HT, and cells were injured after DOX treatment. DOX cytotoxicity enhancement with HT was considered a result of ABCG2 expression downregulation via the increase of ROS production. HT increased intracellular ROS production and downregulated ABCG2 protein expression, leading to cell damage enhancement via DOX.     

Relative abundance of mosquitoes and snails associated with water hyacinth and hippo grass in the Nyanza gulf of Lake Victoria

This study was conducted from September to December 2008 to investigate the relative abundance of malaria vectors and schistosomiasis host snails associated with aquatic weeds in Nyanza Gulf (Lake Victoria). Larval and adult’s stages of mosquitoes, lakeflies and snails were collected and identified with standard entomological and malacological techniques. The relative species composition and abundance of fish associated with macrophytes were also determined. Physico‐chemical parameters were determined with standard analytical methods. Community‐based surveys were also conducted, using standard questionnaires, focused group discussions and direct observations. The results of this study indicated that the abundance of malaria‐causing mosquitoes was low, accounting for only 0.4% of the total number of mosquitoes and lake flies collected from the gulf. Lake flies (Chaoborus and Chironomus spp.) were the most abundant flying insects associated with aquatic macrophytes (84.2%), followed by Culicines Culex spp. (12.2%) and Aedes spp. mosquitoes (3.2%). Biomphalaria sudanica and Bulinus africanus, the two most common hosts for schistosomiasis in the gulf, were detected in both types of macrophytes, but were most significantly attached to water hyacinth (P < 0.0001) and hippo grass (P = 0.0003). There were significantly fewer snails attached to the hippo grass, compared with those unattached in the open water (P < 0.05, GENMOD). Different habitats exhibited low Secchi disc transparency values, but elevated total phosphorous (TP), total nitrogen (TN), chlorophyll‐a concentrations, as well as algal cell counts. Furthermore, Oreochromis niloticus and Haplochromine fishes were more abundant in water hyacinth mats compared with hippo grass mats and open‐water habitats. The low mosquito abundance indicated that the sampled habitats were unsuitable for mosquito breeding, likely attributable to water turbulence and/or predation by larvivorous fish. The strong association between B. sudanica and B. africanus and aquatic macrophytes, and the observation that local communities perform many lakeshore‐related activities that bring them into contact with water, can potentially lead to a higher prevalence of schistosomiasis in the Nyanza Gulf region.     

Systemic and mucosal immune responses of mice to aluminium-adsorbed or aluminium-non-adsorbed tetanus toxoid administered intranasally with recombinant cholera toxin B subunit

For the purpose of changing the immunization procedure of tetanus toxoid from intramuscular or subcutaneous injection, which has been in practice for a long time, to intranasal administration, we examined systemic and mucosal immune responses of mice to aluminium-adsorbed tetanus toxoid (aTT) and aluminium-non-adsorbed tetanus toxoid (nTT) inoculated intranasally with recombinant cholera toxin B subunit (rCTB). Intranasal immunization with aTT induced, at a concentration of 0.5 Lf, high levels of TT-specific serum IgG antibody titres and moderate levels of TT-specific serum IgA antibody titres in the presence and absence of rCTB. Induction of high or moderate levels of mucosal TT-specific IgA antibody responses was observed with and without rCTB in the lung, the nasal cavity, the small and large intestines and the vagina. Generally speaking, the co-administration of aTT and rCTB showed higher mucosal TT-specific IgA antibody titres when compared with the administration of aTT alone. In case of intranasal administration of nTT, the dose of 5 Lf was necessary and stimulated, only in the presence of rCTB (10 micrograms), high levels of tetanus toxoid (TT)-specific serum IgG antibody responses in all mice examined and moderate or slight levels of TT-specific IgA antibody responses in the nasal, pulmonary and small and large intestinal lavages of a few mice. All mice intranasally immunized with aTT alone or nTT and rCTB escaped onset of tetanus. This is the first report concerned with the mucosal adjuvant activity of an aluminium compound. Judging from these results, intranasal administration of aTT with and without rCTB or nTT with rCTB appears to be a very useful means for a vaccination against tetanus with respect to ease, safety, certainty, low cost and no need for an injection needle.