Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle

The actions of prolactin (PRL) on target cells depend on the type of prolactin receptor (PRLr) predominantly expressed, particularly whether the long PRLr isoform is expressed. The aims of this study were to determine the cellular localization and the changes in expression of long and short PRLr isoforms in sheep ovary throughout the estrous cycle. Long and short PRLrs were localized mostly in the same ovarian cells. Maximum signal intensity, particularly for long PRLrs, was found in stromal cells surrounding primordial and primary follicles, and, for both PRLrs, in granulosa cells of preantral follicles and in luteal cells. Moderate signal intensity for PRLrs was found in theca cells of preantral to ovulatory follicles, and in granulosa cells of antral follicles up to the gonadotropin-dependent stage. Decreasing immunoreactivity to PRLrs was found in granulosa cells of gonadotropin-dependent to ovulatory follicles. For long PRLrs in particular, no signal was found in mural granulosa cells of gonadotropin-dependent follicles; for both isoforms, no signal was found in most granulosa cells of ovulatory follicles. In primordial to gonadotropin-dependent follicles, cellular localization of PRLr was similar on days 0, 10 and 15 of the cycle. Oocytes consistently showed positive immunostaining for PRLrs. Comparative RT-PCR analysis of long and short PRLr expression showed that the short isoform is evenly expressed throughout the estrous cycle, whereas the expression of the long form increases at the time of estrus and decreases at mid-luteal phase and at the onset of the follicular phase. Expression of long PRLrs was greater than that of short PRLrs on day 0 of cycle; expression of both isoforms was similar on day 10 and on day 15, long PRLrs expression was lower than that of short PRLrs. Our results indicate that in sheep ovary, the maximum responsiveness to PRL might occur during the preovulatory phase of the estrous cycle.     

Morphological evaluation of bioartificial hydrogels as potential tissue engineering scaffolds

Poly(vinyl alcohol) hydrogels prepared by freeze-thawing procedure represent synthetic systems widely investigated as non-biodegradable scaffolds for tissue regeneration. In order to improve the biocompatibility properties of pure poly(vinyl alcohol) (PVA) hydrogels, blends of PVA with different biological macromolecules, such hyaluronic acid, dextran, and gelatin were prepared and used to produce bioartificial hydrogels. The porosity characteristics of these hydrogels were investigated by scanning electron microscopy and mercury intrusion porosimetry. The morphology of bioartificial hydrogels was evaluated and compared with that of pure PVA hydrogels. In particular the effect exerted by each biological component on pore size and distribution was investigated. The obtained results indicate that when a natural macromolecule is added to PVA the internal structure of the material changes. A small amount of biopolymer induces the structural elements of PVA matrix to take on a well evident lamellar appearance and an apparent preferential orientation. Comparing the results of SEM and mercury intrusion porosimetry it was concluded that hydrogels containing 20% of biological component have the most regular structure and at the same time the lowest total porosity. On the contrary samples with the highest content of natural polymer (40%) show the less regular structure and the highest total porosity.     

The raison d'être of constitutively active protein kinases: the lesson of CK2

Protein kinases generally are tightly controlled signaling molecules that are switched on only in response to specific stimuli. Exceptionally few protein kinases are constitutively active, the most striking example being provided by CK2 (formerly "casein kinase 2"). Owing to unique structural features, the catalytic activity of CK2 is constantly on, although its targeting can be deeply influenced by the association of its two catalytic subunits (alpha and/or alpha') with a dimeric non catalytic beta subunit. Constitutive activity of CK2 reflects its extraordinary pleiotropy documented by its growing list of >300 protein substrates and is consistent with emerging evidence that CK2 plays an essential role in the cell by counteracting premature and/or unscheduled apoptosis, thus ensuring cell survival under stress conditions.     

An Integrated CMOS Front-End for Optical Absolute Rotary Encoders

This paper presents a front-end circuit for optical rotary encoders. The light pulses modulated by the encoder disc are transduced into current signals, which are pre-processed and converted into digital waveforms related to the disc angular position information. The proposed front-end circuit is compensated against temperature drifts. Digitally programmable calibration is provided to account for spreads in impinging light pulse power. Measurement results on integrated prototypes are shown, demonstrating correct operation of the front-end with an optical input power from 0.5 W to 3 W up to a signal frequency of 500 kHz in a temperature range from 0 °C to 80 °C.     

Optimisation of the throttling system in a CO2 refrigerating machine

This paper is an answer to the need of finding the optimal solution for the throttling system in refrigerating machines using CO₂ as working fluid; such a solution must combine reliability, low installation cost and high energy efficiency. To this purpose, different expansion systems are compared by means of a simulation programme, including a new one, operating with a differential valve, a liquid receiver and a thermostatic valve. The typical compression refrigerating cycle performed by CO₂ involves transcritical operations and therefore the upper pressure needs to be adjusted to the optimal value, that, unlike the traditional cycle, is not determined by heat transfer. The innovative system here proposed shows an intrinsic self-adjusting capability that leads to COP values quite close to the maximum ones when a fixed suitable value of the differential pressure is chosen, even if the temperature of the secondary fluid varies to a large extent.     

Squaring down LTI systems: A geometric approach

In this paper, the problem of reducing a given LTI system into a left or right invertible one is addressed and solved with the standard tools of the geometric control theory. First, it will be shown how an LTI system can be turned into a left invertible system, thus preserving key system properties like stabilizability, phase minimality, right invertibility, relative degree and infinite zero structure. Moreover, the additional invariant zeros introduced in the left invertible system thus obtained can be arbitrarily assigned in the complex plane. By duality, the scheme of a right inverter will be derived straightforwardly. Moreover, the squaring down problem will be addressed. In fact, when the left and right reduction procedures are applied together, a system with an unequal number of inputs and outputs is turned into a square and invertible system. Furthermore, as an example it will be shown how these techniques may be employed to weaken the standard assumption of left invertibility of the plant in many optimization problems.     

Simulation of the St. Francis Dam-Break Flood

A two-dimensional (2D) simulation of flooding from the 1928 failure of St. Francis Dam in southern California is presented. The simulation algorithm solves shallow-water equations using a robust unstructured grid Godunov-type scheme designed for wetting and drying and achieves good results. Flood extent and flood travel time are predicted within 4 and 10% of observations, respectively. Representation of terrain by the mesh is identified as the dominant factor affecting accuracy, and an iterative process of mesh refinement and convergence checks is implemented to minimize errors. The most accurate predictions are achieved with a uniformly distributed Manning n = 0.02. A 50% increase in n increases travel time errors to 25% but has little effect on flood extent predictions. This highlights the challenge of a priori travel time prediction but robustness in flood extent prediction when topography is well resolved. Predictions show a combination of subcritical and supercritical flow regimes. The leading edge of the flood was supercritical in San Francisquito Canyon, but due to channel tortuosity, the wetting front reflected off canyon walls causing a transition to subcritical flow, considerably larger depths, and a standing wave in one particular reach that accounts for a 30% fluctuation in discharge. Elsewhere, oblique shocks locally increased flood depths. The 2D dam-break model is validated by its stability and accuracy, conservation properties, ability to calibrate with a physically realistic and simple resistance parametrization, and modest computational cost. Further, this study highlights the importance of a dynamic momentum balance for dam-break flood simulation.     

Optical and Structural Properties of CdTe Grown by Molecular Beam Epitaxy at Low Temperature for Resonant-Cavity-Enhanced HgCdTe Detectors

Investigation into resonant-cavity-enhanced (RCE) HgCdTe detectors has revealed a discrepancy in the refractive index of the CdTe layers grown by molecular beam epitaxy (MBE) for the detectors, compared with the reported value for crystalline CdTe. The refractive index of the CdTe grown for RCE detectors was measured using ellipsometry and matches that of CdTe with an inclusion of approximately 10% voids. X-ray measurements confirm that the sample is crystalline and strained to match the lattice spacing of the underlying Hg_(1−x)Cd_(x)Te, while electron diffraction patterns observed during growth indicate that the CdTe layers exhibit some three-dimensional structure. Secondary ion mass spectroscopy results further indicate that there is enhanced interdiffusion at the interface between Hg_(1−x)Cd_(x)Te and CdTe when the Hg_(1−x)Cd_(x)Te is grown on CdTe, suggesting that the defects are nucleated within the CdTe layers.     

Skew product cycles with rich dynamics: From totally non-hyperbolic dynamics to fully prevalent hyperbolicity

We introduce a two-parameter family of ‘partially hyperbolic’ skew products (G_{a, t})_{a > 0, t ∈ [ ? ε, ε]} maps with one dimensional centre direction. In this family, the parameter a models the central dynamics and the parameter t the unfolding of cycles (that occurs for t = 0). The parameter a also measures the ‘central distortion’ of the systems: for small a, the distortion of the systems is small and it increases and goes to infinity as a → ∞. The family (G_{a, t}) displays some of the main characteristic properties of the unfolding of heterodimensional cycles as intermingled homoclinic classes of different indices and secondary bifurcations via collision of hyperbolic homoclinic classes. For a ∈ (0, log?2), the dynamics of (G_{a, t}) is always non-hyperbolic after the unfolding of the cycle. However, for a > log?4 intervals of t-parameters corresponding to hyperbolic dynamics appear and turn into totally prevalent as a → ∞ (the density of ‘hyperbolic parameters’ goes to 1 as a → ∞). The dynamics of the maps G_{a, t} is described using a family of iterated function systems modelling the dynamics in the one-dimensional central direction.     

Playing MUSIC — building context‐aware and self‐adaptive mobile applications

Although the idea of context‐awareness was introduced almost two decades ago, few mobile software applications are available today that can sense and adapt to their run‐time environment. The development of context‐aware and self‐adaptive applications is complex and few developers have experience in this area. On the basis of several demonstrators built by the joint European research project MUSIC, this paper describes typical context and adaptation features relevant for the development of context‐aware and self‐adaptive mobile applications. We explain how the demonstrators were realised using the open‐source platform MUSIC and present the feedback of the developers of these demonstrators. The main contribution of this paper is to show how the development complexity of context‐aware and self‐adaptive mobile applications can be mastered by using an adaptation framework such as MUSIC. Copyright © 2012 John Wiley & Sons, Ltd.