Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber‐Initiated Controlled Radical Polymerization

Native tissues are typically heterogeneous and hierarchically organized, and generating scaffolds that can mimic these properties is critical for tissue engineering applications. By uniquely combining controlled radical polymerization (CRP), end‐functionalization of polymers, and advanced electrospinning techniques, a modular and versatile approach is introduced to generate scaffolds with spatially organized functionality. Poly‐ε‐caprolactone is end functionalized with either a polymerization‐initiating group or a cell‐binding peptide motif cyclic Arg‐Gly‐Asp‐Ser (cRGDS), and are each sequentially electrospun to produce zonally discrete bilayers within a continuous fiber scaffold. The polymerization‐initiating group is then used to graft an antifouling polymer bottlebrush based on poly(ethylene glycol) from the fiber surface using CRP exclusively within one bilayer of the scaffold. The ability to include additional multifunctionality during CRP is showcased by integrating a biotinylated monomer unit into the polymerization step allowing postmodification of the scaffold with streptavidin‐coupled moieties. These combined processing techniques result in an effective bilayered and dual‐functionality scaffold with a cell‐adhesive surface and an opposing antifouling non‐cell‐adhesive surface in zonally specific regions across the thickness of the scaffold, demonstrated through fluorescent labelling and cell adhesion studies. This modular and versatile approach combines strategies to produce scaffolds with tailorable properties for many applications in tissue engineering and regenerative medicine.     

The Deliberative Politics of the Consultative Layer: Participation Hopes and Communication as Design Values of Civic Tech Founders

Drawing from communication as design and the spirit of technology, this study investigated the political values embedded in consultative layer companies, with particular attention to the influence of normative deliberative democratic ideals in tech design. Interviews with the founders of consultative layer tech startups explored (a) founders' visions for their technologies and how they were incorporated, (b) the imagined user groups for these technologies, and (c) expected broader outcomes as a result of using their platforms. Six interrelated themes emerged in the analysis that illuminate ideologies, ideals, and pragmatic considerations embedded in the consultative layer, raising new theoretical and practical questions about the role of communication in understanding this emerging industry and its reimagining of government in the online space.     

A simple iterative algorithm for parsimonious binary kernel Fisher discrimination

By applying recent results in optimisation theory variously known as optimisation transfer or majorise/minimise algorithms, an algorithm for binary, kernel, Fisher discriminant analysis is introduced that makes use of a non-smooth penalty on the coefficients to provide a parsimonious solution. The problem is converted into a smooth optimisation that can be solved iteratively with no greater overhead than iteratively re-weighted least-squares. The result is simple, easily programmed and is shown to perform, in terms of both accuracy and parsimony, as well as or better than a number of leading machine learning algorithms on two well-studied and substantial benchmarks.     

Nonparabolicity effects and the spin-split electron dwell time in symmetric III-V double-barrier structures

We start from the fourth-order nonparabolic and anisotropic conduction band bulk dispersion relation to obtain a one-band effective Hamiltonian, which we apply to an AlGaSb symmetric double-barrier structure with resonant energies significantly (more than 200 meV) above the well bottom. The spin-splitting is described by the k³ Dresselhaus spin-orbit coupling term modifying only the effective mass of the spin eigenstates in the investigated structure. Apart from the bulk-like resonant energy shift due to the band nonparabolicity, we obtain a substantial shift depending on the choice of boundary conditions for the envelope functions at interfaces between different materials. The shift of resonant energy levels leads to the change of spin-splitting and the magnitude of the dwell times. We attempt to explain the influence of both the nonparabolicity and boundary conditions choice by introducing various effective masses.     

The role of docosahexaenoic acid in retinal function

An important role for docosahexaenoic acid (DHA) within the retina is suggested by its high levels and active conservation in this tissue. Animals raised on n-3-deficient diets have large reductions in retinal DHA levels that are associated with altered retinal function as assessed by the electroretinogram (ERG). Despite two decades of research in this field, little is known about the mechanisms underlying altered retinal function in n-3-deficient animals. The focus of this review is on recent research that has sought to elucidate the role of DHA in retinal function, particularly within the rod photoreceptor outer segments where DHA is found at its highest concentration. An overview is also given of human infant studies that have examined whether a neonatal dietary supply of DHA is required for the normal development of retinal function.     

Setting performance criteria for building products

As previously reported in our pages, the CIB working group on the performance concept in building, W60, has produced a booklet of six papers, each dealing with setting performance criteria for building products. They present alternative methodologies and are not intended to provide detailed guidance for the values to be specified. The document is summarised and some of the major issues involved are outlined in this article. There is only one fundamental criterion of success, it is concluded, namely the correct matching of the solution to the originally-defined user needs. The different methods discussed are all means to that end.     

Electrospinning formaldehyde‐crosslinked zein solutions

In order to develop zein fibers with improved physical properties and solvent resistance, formaldehyde was used as a crosslinking reagent before spinning. The crosslinking reaction was carried out in either acetic acid or ethanolic HCl where the amount of crosslinking reagent was between 1 and 6%. Reactions were carried out at various times and temperatures. When carried out in acetic acid, the maximum amount of formaldehyde that could be used was 1.5% as gelation occurred at higher levels. In ethanolic HCl, 6% formaldehyde could be incorporated into zein. All solutions were successfully electrospun, producing predominantly ribbon and round fibers; the morphology was strongly dependent on solvent and spinning solids. The formaldehyde‐crosslinked zein fabrics had double the tensile strength of control fabrics. SDS‐PAGE analysis clearly showed crosslinking had occurred. Electrospun fabrics from all formaldehyde‐treated zein solutions required an additional heating step in order to be resistant to dissolution in acetic acid, a known very good solvent for zein. Copyright © 2011 Society of Chemical Industry     

Experimental Characterization and Optimization of Hybrid FRP/RC Bridge Superstructure System

An experimental investigation was performed to assess the performance of a hybrid fiber-reinforced polymer/reinforced concrete bridge system. The full-scale laboratory specimen was representative of an 813?mm (32?in.) wide strip of a completed bridge in San Patricio County, Tex. The specimen was first subjected to static loading prior to casting the reinforced concrete deck. Displacement, strain, and acoustic emission were recorded. After completion of the nondestructive static loading a reinforced concrete deck was cast in the laboratory to represent one unit of the completed bridge. Load was statically applied with several increased load cycles until failure occurred at a load level exceeding 18 times the calculated design load. The results of the static testing indicated that the original design of the hybrid bridge was very conservative. An optimized design of the hybrid bridge was then derived. The static load testing program and the resulting optimized design are described.