An optimal model for representing the kinetics of growth and product formation by Lactobacillus rhamnosus on multiple substrates

A comprehensive model was developed to simulate Lactobacillus rhamnosus growth on a medium containing multiple limiting carbon sources. The strategy of optimizing specific growth rate to predict growth on multiple substrates was demonstrated. The model predictions were based on parameters obtained from L. rhamnosus growth on individual substrates. The model was able to simulate the growth, substrate consumption, product formation and specific growth rate profiles of L. rhamnosus accurately. The model prediction that co-metabolism of glucose and pyruvate enhances growth rate of and flavor production by the bacterium was experimentally verified.     

Ascorbic acid-mediated synthesis and characterisation of iron oxide/gold core–shell nanoparticles.

The current article reports on providing surface modification of magnetic nanoparticles with gold to provide stability against aggregation. Gold-coated magnetite nanoparticles were synthesised to combine both magnetic as well as surface plasma resonance (SPR) properties in a single moiety. The nanocomposites were produced by reduction (using ascorbic acid) of gold chloride on to the surface of iron oxide nanoparticles. Ascorbic acid not only acts as a reducing agent, but also the oxidised form of ascorbic acid i.e. Dehydro-ascorbic acid acts as a capping agent to impart stability to as synthesised gold-coated iron oxide nanocomposites. The synthesised nanocomposite was monodispersed with a mean particle size of around 16 nm and polydispersity index of 0.190. X-ray diffraction analysis confirms presence of gold on the surface of magnetite nanoparticles. The synthesised nanocomposites had a total organic content of around 3.2% w/w and also showed a shifted SPR peak at 546 nm as compared to gold nanoparticles (528 nm). Both uncoated and gold-coated magnetite exhibited superparamagnetic behaviour at room temperature. Upon coating with gold shell, saturation magnetisation of iron oxide nanoparticles decreases from 42.806 to 3.54 emu/gram.     

Above ground standing biomass and carbon storage in village bamboos in North East India

Bamboo forms an important component in the traditional landscape of North East India. For biomass estimation of village bamboos of Barak Valley, North East India, allometric relationships were developed by harvest method describing leaf, branch and culm biomass with DBH as an independent variable using a log linear model. The culm density of the stand was 8950 culms ha⁻¹ during 2005 of which 67% of growing stock was represented by Bambusa cacharensis, 17.88% by Bambusa vulgaris and 15.12% by Bambusa balcooa. Above ground stand biomass was 121.51 t ha⁻¹ of which 86% was contributed by culm component followed by branch (10%) and leaf (4%). With respect to species, B. cacharensis made up to 46% of total stand biomass followed by B. vulgaris (28%) and B. balcooa (26%). Carbon storage in the above ground biomass was 61.05 t ha⁻¹. Allocation of C was more in culm components (53.05 t ha⁻¹) than in branch (5.81 t ha⁻¹) and leaf (2.19 t ha⁻¹). Carbon storage in the litter floor mass was 2.40 t ha⁻¹, of which leaf litter made up the highest amount (1.37 t ha⁻¹) followed by sheath (0.86 t ha⁻¹) and branch (0.17 t ha⁻¹). Carbon stock in the soil up to 30 cm depth was 57.3 t ha⁻¹. Gross C stock in the plantation was estimated to be 120.75 t ha⁻¹. Carbon storage estimated in the bamboo stand of present study offers insights into the opportunity of village bamboos in the rural landscape for carbon storage through carbon sequestration. Management and utilization of village bamboos as a potential source of carbon sink by smallholder farmers are discussed in the context of their livelihood security and the Millennium Development Goals of the United Nations.     

Ambient pressure dried TEOS-based silica aerogels: good absorbents of organic liquids

The experimental work has been carried out to study the absorption–desorption properties of the hydrophobic silica aerogel for the application of oil spill cleanup. Aerogels were synthesized by sol–gel route prior to ambient pressure drying, by keeping TEOS:MeOH:Acidic H₂O:NH₄F:NH₄OH:HMDZ molar ratio constant at 1:16.5:0.81:0.62:0.63:0.41, respectively. The absorption of organic liquids by as-prepared aerogels has been carried out by adding the aerogel samples in organic liquid, viz. three alkanes: hexane, heptane, octane; the aromatic compounds: benzene, toluene, xylene; the alcohols: methanol, ethanol, propanol, and the three oils: petrol, diesel, and kerosene until it is totally wetted. It was observed that surface-modified TEOS-based aerogel absorbed the organic liquids and oils by nearly 12 times its own mass. After absorption, desorption time of liquids from the aerogel at various temperatures, i.e., at 30, 60, 80, and 100 °C was measured. The 50% volume shrinkage of the aerogel in case of oils and 20% in case of organic liquid was observed, after total desorption of liquids. No significant change in hydrophobicity of the aerogel was observed and it can be reused two times.     

Contamination of surface and potable water in South Asia by Salmonellae: Culture-independent quantification with molecular beacon real-time PCR

Low numbers (15-100 CFU) of Salmonella in food or water may pose a public health risk. The management of infections caused by Salmonella spp. during outbreaks or forecasting of contamination of aquatic resources largely depends on rapid, sensitive and accurate diagnostic in few hours. In this study, a real-time PCR assay in Molecular-Beacon format was developed and culture-independent quantitative enumeration of Salmonella spp. in surface and potable water is being reported for the first time from northern part of India. Molecular Beacon was designed in highly conserved region of invA gene (present in wide range of Salmonella serotypes including all subspecies) encoding an essential component of the invasion associated specialized type Ø protein secretion apparatus for detection of Salmonella spp. in water. The assay could detect directly 10 and 1 genomic equivalent of Salmonella typhimurium ATCC 14028 per PCR with detection probability of 100 and 20%, respectively. Further, the assay could detect 10 CFU/PCR or more of reference strain (S. typhimurium ATCC 14028) without any enrichment in the presence of 10⁸ CFU ml^− 1 of non-pathogenic E. coli (E. coli DH5α) with 100% detection probability. The assay could enumerate Salmonella spp. in surface (n = 40) and potable waters (n = 10) directly (without enrichment). Results indicate that northern India is at high risk of developing Salmonella borne infections. Further, real-time PCR assay in Molecular Beacon format can be used for identification of critical contamination points in natural water resources and potable water distribution systems, necessary to implement vaccination plan timely for prevention of waterborne outbreaks caused by Salmonella spp.     

Parametric Study of Jatropha Seeds for Biodiesel Production by Reactive Extraction

The purpose of the present study was to reduce the cost and increase the efficiency of biodiesel production by reactive extraction (in situ) of Jatropha seeds. Oil from the seeds was extracted and reacted in a single step. Experimental studies have been carried out to maximize the yield of biodiesel by varying the reaction parameters viz. seed size (<0.85 mm to >2.46 mm), seed/solvent ratio (w/w) (1:2.6–1:7.8) and catalyst concentration (0.05–0.1 M). Under the optimized conditions: seed size (>2.46 mm), seed/solvent ratio (w/w) (1:7.8), catalyst concentration (0.1 M) and reaction time 1 h, approximately 98% conversion to biodiesel was achieved meeting International (ASTM) as well as National (BIS) specifications. The results were supported by HPLC analysis.     

Identification of yeast strains isolated from marcha in Sikkim, a microbial starter for amylolytic fermentation

Marcha or murcha is a traditional amylolytic starter used to produce sweet-sour alcoholic drinks, commonly called jaanr in the Himalayan regions of India, Nepal, Bhutan, and Tibet (China). The aim of this study was to examine the microflora of marcha collected from Sikkim in India, focusing on yeast flora and their roles. Twenty yeast strains were isolated from six samples of marcha and identified by genetic and phenotypic methods. They were first classified into four groups (Group I, II, III, and IV) based on physiological features using an API test. Phylogenetic, morphological, and physiological characterization identified the isolates as Saccharomyces bayanus (Group I); Candida glabrata (Group II); Pichia anomala (Group III); and Saccharomycopsis fibuligera, Saccharomycopsis capsularis, and Pichia burtonii (Group IV). Among them, the Group I, II, and III strains produced ethanol. The isolates of Group IV had high amylolytic activity. Because all marcha samples tested contained both starch degraders and ethanol producers, it was hypothesized that all four groups of yeast (Group I, II, III, and IV) contribute to starch-based alcohol fermentation.     

Effect of support materials on the performance of direct ethanol fuel cell anode catalyst

Pt–Ru catalysts supported on mesoporous carbon nitride (MCN), multiwall carbon nano tubes (MWCNTs), treated MWCNTs (t-MWCNTS) and Vulcan-XC were prepared using co-impregnation reduction method for the oxidation of ethanol in direct ethanol fuel cell (DEFC) to study the effect of support material. The MCN support was prepared using SBA-15 as template and t-MWCNTs were prepared by refluxing in HNO₃ and H₂SO₄ mixture (1:3) using MWCNTs. XRD shows the formation of Pt–Ru bi-metallic catalyst with size ranges from 7 to 17 nm using different supports. The catalyst and its supports were characterized by physically and electrochemically. Linear sweep voltammetry, cyclic voltammetry and chrono amperometry studies of the above systems reveal that MCN supported Pt–Ru catalyst shows higher electro-catalytic activity towards ethanol oxidation compared to Pt–Ru in treated t-MWCNTs, MWCNts and Vulcan-XC supports. The performance of DEFC based on maximum power density is found to be in the order Pt–Ru/MCN > Pt–Ru/t-MWCNTs > Pt–Ru/MWCNTs > Pt–Ru/Vulcan-XC. The Pt–Ru/MCN shows highest power density of 61.1 mW cm⁻² at 100 °C, 1 bar pressure with catalyst loading of 2 mg cm⁻² using 2 M ethanol feed.     

Combined experimental and analytical method for a realistic seismic qualification of equipment

The seismic qualification of equipment/structures are, in general, carried out either exclusively by analysis or exclusively by testing using a shake table. The analytical methods have the risk of the model not being a true reflection of the structure unless very elaborate modelling techniques are used. Even with an elaborate model there are many idealisations made which may not actually be realised in practice. The shake-table testing, avoids the modelling deviations to a large extent, but is also not without drawbacks. The important ones are the cost and the availability of a shake table of the required size and capacity. The shake-table testing is also carried out on the isolated equipment without the piping/structural connections from other components. The present paper suggests a combined experimental and analytical method on the ‘as installed’ equipment as an attractive alternative which overcomes the above drawbacks. In contrast to the existing practice of using the experimental results just to validate the analytical model, the suggested method uses the experimentally obtained dynamic characteristics of the ‘as installed’ equipment to obtain the response to the design seismic load. The paper brings out through an example of a simple storage tank which is too heavy for a shake table, the large deviations in its actual behaviour vis-à-vis an idealised analytical model.