Experimental investigation and biomechanical analysis of lower limb movements for clutch pedal operation

Lower limb movements for clutch-pedal operations were investigated and the influence of four parameters (seat height, pedal travel, pedal travel inclination, pedal resistance) was studied using a multi-adjustable experimental seat. Fifteen subjects participated in the experiment: five short females, five average height males and five tall males. A biomechanical model has been proposed to explain how pedal force direction could be controlled. The experimental observations show that the lower limb movement of clutch pedal operation is mainly guided by the geometric constraints imposed by the task and its environment, especially during the depression phase. The results support the hypothesis that movements obey the principle of minimum work and minimum discomfort. Furthermore, it seems that a functional segmentation exists between the distal joint (ankle) and the proximal joints (knee and hip), thus simplifying the control problem, which is due to the redundancy of the human body. It appears that the depression movement is controlled by proprioceptive feedback related to foot displacement and pedal force, from the fact that the deceleration duration during the depression phase increases with the pedal resistance and pedal travel. The minimum pedal resistance and pedal travel are discussed.     

Variations of anatomical elements contributing to subtalar joint stability: intrinsic risk factors for post-traumatic lateral instability of the ankle?

Ankle sprains are frequently followed by chronic lateral instability, often with talar hypermobility. This might be due to subtalar instability. Among intrinsic risk factors, anatomical variants are generally overlooked. In the subtalar region, anatomical variation is particularly frequent. On the talus as well as on the calcaneus, the anterior articular facets may be missing or fused with the medial facets, giving rise to three subtalar joint configurations: a three-joint configuration, a fused configuration with a relatively large anteromedial joint, and a two-joint configuration without anterior joint. Osteometry was performed on these joint facets (134 calcanei, 122 tali), demonstrating significant differences in the surface of these configurations and the existence of a supplementary supporting surface with grossly transverse orientation in the three-joint configuration. There are also several variants of stabilizing ligaments within the sinus tarsi. Some of these configurations might expose to increased risk of associated subtalar lesions, resulting in subtalar instability. A systematic look for these variants is recommended in order to evaluate the associated risk factors, eventually resulting in a better understanding, prevention and cure of sequellae.     

Implications of an adjustable bed height during standard nursing tasks on spinal motion, perceived exertion and muscular activity

Manual handling is a source of occupational stress, particularly for nursing personnel. High levels of biomechanical strain are associated with lifting and transferring patients, especially when the tasks are performed in flexed and twisted positions that induce an increased risk of functional and musculoskeletal problems. The use of adjustable beds in nursing practice has been suggested as a means of influencing working postures and reducing the muscular demands on nurses. The purpose of this study was to investigate the effects on spinal motion, muscular activity and perceived exertion when nurses had the opportunity to adjust bed height. The measures recorded during the conduct of standardized patient handling tasks were the changes in posture (inclination) and in shape (sagittal bending, side bending, axial rotation). Muscular activity was measured using surface electromyography. Perceived exertion was rated using the 15-graded Borg scale. The range of motion was not influenced by the adjustment of bed height, but rather a shift of the time duration histogram was noticed in the direction of the erect, safer position. The time spent in the safe zone of spinal motion near the erect position was significantly increased and was significantly decreased in the potential health-hazardous zones of spinal motion in the extreme positions. No differences in muscular activity or in perceived exertion were found between the two bed height conditions for any of the muscle groups. It was concluded that the quality of spinal motion is enhanced when the opportunity of adjusting the bed height is offered.     

Impact forces following the unexpected removal of a chair while sitting

This report documents the impact forces measured during trials of dropping an anthropometric dummy (80.3 kg) (Hybrid III, First Technologies Corporation) in three different positions onto it's pelvis (gluteal region) from a seated height, which was meant to simulate a chair being pulled out from an individual in the process of sitting. Peak forces on the pelvis were measured by a force plate covered with industrial carpet. These impact forces were translated to the compressive and shear forces on the lumbar spine. The peak impact forces during the different body postures were 20000-29900 N (torso upright); 13000-22200 N (torso-legs 45 degrees to floor); 6000-15200 N (layout position). The impact forces generated from falling onto the pelvis from a seated height, appear to be sufficient to cause injury as the forces well exceed documented injury tolerance levels.     

Release of benzimidazole and benzylidene camphor from topical sunscreen formulations

Absorption of two ultraviolet (UV) filters was evaluated through a lipophilic synthetic membrane (Folioxane®) and excised hairless rat skin using a flow-through diffusion cell. Folioxane membrane is an artificial skin used in the treatment of third-degree burns. Diffusion tests were performed with aqueous solutions and galenic formulations (one water-in-oil [W/O] emulsion and two oily gels). Analyses were achieved with high-performance liquid chromatography (HPLC) with UV detection at 295 nm. Diffusion kinetics of 17 β estradiol, a reference compound, through rat skin, human skin, and Folioxane membrane were performed to validate the in vitro model. Phenylbenzimidazole and methylbenzylidene camphor in aqueous solutions were diffused at a regular rate through the Folioxane film. The release of phenylbenzimidazole was very slow, whereas the release of benzylidene camphor was more pronounced: a decrease of the quantity was observed in the donor compartment (30 % at 6 hr and 93% after 72 hr). A significant flow of benzylidene camphor was also measured through excised skin of rat in the first 3 hr. The skin absorption was 38% over 72 hr. The W/O emulsion had low penetration of UV filter: 20% of the initial amount for Folioxane membrane and 0.4% for rat skin. In contrast, the penetration of two oily gels was identical: 28% on Folioxane membrane and 0.6% on rat skin. This study demonstrates the transcutaneous diffusion of two important classes of sunscreens through a lipophilic Folioxane membrane and through excised hairless rat skin. From the results, Folioxane membrane appears to be an alternative model for studying diffusion of topical molecules and as a tool for guiding formulation choices.     

Thin films of calcium phosphate and titanium dioxide by a sol-gel route: a new method for coating medical implants

Titanium is a commonly used biomaterial for dental and orthopaedic applications. To increase its ability to bond with bone, some attempts were made to coat its surface with calcium phosphate (CaP). This paper describes a new type of coating. Instead of a pure CaP layer, a mixing of titanium dioxide (TiO₂) and CaP is fabricated and deposited as a coating. These layers are deposited by a sol-gel route on pure titanium substrates using various pre-treatments. The method consists of mixing a solution of tetrabutyl ortho-titanate or a sol of titanium dioxide with a solution of calcium nitrate and phosphorous esters. This composite is deposited on to commercially pure titanium plates, mechanically polished or blasted with pure crystalline aluminum oxide, using the spin-coating technique. These coatings are then fired at 650 or 850°C for various times. The samples are characterized by X-ray diffraction for their crystallinity, X-ray photoelectron spectroscopy for their surface chemical composition and scanning electron microscopy for their topography. Samples treated at 850°C present a well-pronounced crystallinity, and a high chemical purity at the surface. The topography is strongly related to the viscosity of the precursor and the substrate pre-treatment. Possibilities to structure the outermost layer are presented. © 1999 Kluwer Academic Publishers     

Significance of Z-value statistics of Smith-Waterman scores for protein alignments

The Z-value is an attempt to estimate the statistical significance of a Smith-Waterman dynamic alignment score (SW-score) through the use of a Monte-Carlo process. It partly reduces the bias induced by the composition and length of the sequences. This paper is not a theoretical study on the distribution of SW-scores and Z-values. Rather, it presents a statistical analysis of Z-values on large datasets of protein sequences, leading to a law of probability that the experimental Z-values follow. First, we determine the relationships between the computed Z-value, an estimation of its variance and the number of randomizations in the Monte-Carlo process. Then, we illustrate that Z-values are less correlated to sequence lengths than SW-scores. Then we show that pairwise alignments, performed on 'quasi-real' sequences (i.e., randomly shuffled sequences of the same length and amino acid composition as the real ones) lead to Z-value distributions that statistically fit the extreme value distribution, more precisely the Gumbel distribution (global EVD, Extreme Value Distribution). However, for real protein sequences, we observe an over-representation of high Z-values. We determine first a cutoff value which separates these overestimated Z-values from those which follow the global EVD. We then show that the interesting part of the tail of distribution of Z-values can be approximated by another EVD (i.e., an EVD which differs from the global EVD) or by a Pareto law. This has been confirmed for all proteins analysed so far, whether extracted from individual genomes, or from the ensemble of five complete microbial genomes comprising altogether 16956 protein sequences.     

Effects of the presence of the mammary gland on expression of neutrophil adhesion molecules and myeloperoxidase activity in periparturient dairy cows

Neutrophil function is reduced in periparturient dairy cows. Possible factors that reduce neutrophil function include endocrine changes associated with parturition and metabolic stresses associated with lactogenesis. In this study, mastectomized and intact cows were studied to specifically examine the effects of lactogenesis on neutrophil function in periparturient cows. Expression of adhesion molecules on neutrophils (L-selectin, mediating capture and rolling adhesion, and beta 2-integrin, mediating tight adhesion vital to egress) and neutrophil myeloperoxidase activity (an index of bactericidal activity) were assessed in mastectomized and intact cows. Expression of L-selectin decreased at parturition followed by rapid recovery to prepartum values in both intact and mastectomized cows. Expression of beta 2-integrins increased in intact cows at parturition but not in mastectomized cows. Expression of beta 2-integrins was greater in intact cows than in mastectomized cows throughout the study. Neutrophil myeloperoxidase activity decreased from baseline prepartum values as parturition approached in both intact and mastectomized cows, which suggests the endocrine changes associated with the act of parturition are predominant factors causing loss of neutrophil function. Myeloperoxidase activity recovered to prepartum values within a week of parturition in mastectomized cows; however, myeloperoxidase activity remained depressed in neutrophils obtained from intact cows throughout the first 20 d of lactation. The presence of the mammary gland and its attendant metabolic stresses slowed recovery of neutrophil function, which suggests that the metabolic stress of lactation exacerbated periparturient immunosuppression.     

How to bring orphan genes into functional families

In the framework of the B1 Consortium of the EUROFAN-1 project, we set up a series of simple phenotypic tests that can be performed on a large number of strains at a time. This methodological approach was intended to help assign functions of putative genes coding for unknown proteins to several specific aspects of cell biology. The tests were chosen to study phenotypes which should be affected by numerous genes. In this report, we examined the sensitivity/resistance or the adaptation of the cell to physical or chemical stresses (thermotolerance, osmotolerance and ethanol sensitivity), the effects of the alteration of the level of protein phosphorylation (sensitivity or resistance to compounds affecting the activity of protein kinases or phosphatases) and the effects of compounds interfering with synthesis of nucleic acids or proteins. Deletions in 66 genes of unknown function have been tested in 21 different conditions. In many deletant strains, phenotypes were observed and, for the most promising candidates, tetrad analysis was performed in order to verify co-segregation of the deletion marker with the phenotype.     

Excess-noise dependence on intracavity aperture shape

In lasers with nonorthogonal eigenmodes the excess-noise factor K can be large, especially in unstable-cavity lasers with hard-edged intracavity apertures. To the best of our knowledge, we report the first detailed study of the dependence of K on aperture shape. Calculations and measurements of K for unstable-cavity lasers with variable-size apertures of triangular, square, pentagonal, hexagonal, octagonal, and rhomboid symmetries are summarized. It is shown that both the magnitude of K and its resonant behavior strongly depend on aperture shape and that many aspects of this dependence can be explained in terms of one-dimensional resonance lengths.