Ex vivo expansion with stem cell factor and interleukin-11 augments both short-term recovery posttransplant and the ability to serially transplant marrow

The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-11 (IL-11) produced a 50-fold amplification of clonogenic multipotential progenitors (CFU-A). Following such ex vivo expansion, substantially fewer cells were required to rescue lethally irradiated mice. When transplanted in cell doses above threshold for engraftment, BM cells expanded ex vivo resulted in significantly more rapid hematopoietic recovery. In a serial transplantation model, unmanipulated BM was only able to consistently sustain secondary BMT recipients, but BM expanded ex vivo has sustained quaternary BMT recipients that remain alive and well more than 140 days after 4th degree BMT. These results show augmentation of both short-term recovery posttransplant and the ability to serially transplant marrow by preincubation in culture with SCF and IL-11.     

弹性筛管防砂技术

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引水隧洞煤系地层塌方冒顶施工处理技术

煤系地层是一种较软弱的岩体，易风化膨胀，稳定性极差，遇水迅速坍塌，进行煤系地层的掘进施工难度大。本文结合大花水水电站引水隧洞实例，介绍了其穿越煤系地层塌方冒顶洞段的施工处理技术。     

平面Z-型软磁铁氧体频率特性的研究

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虚拟装配环境中的一种碰撞干涉检测方法

提出了在虚拟装配过程中零件与装配体碰撞干涉检测的一种方法。用投影轴算法检测出装配对象的碰撞,用坐标跟踪检测方法确定干涉余量值,并用连续包围盒对复杂和不规则的模型进行分割,通过这三种方法的配合使用,虚拟装配系统对干涉体进行了有效的、实时的、精确的检测。     

浅谈EPC总承包工程项目质量管理

结合EPC工程项目管理特点，将质量管理的基本要素充分体现在每一个工作环节，确保工程质量符合规程规范和业主质量目标要求，为机组今后正常稳定运行奠定坚实的基础。     

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