全文获取类型
收费全文 | 6518篇 |
免费 | 325篇 |
国内免费 | 33篇 |
学科分类
医药卫生 | 6876篇 |
出版年
2023年 | 51篇 |
2022年 | 96篇 |
2021年 | 143篇 |
2020年 | 91篇 |
2019年 | 106篇 |
2018年 | 113篇 |
2017年 | 98篇 |
2016年 | 115篇 |
2015年 | 135篇 |
2014年 | 159篇 |
2013年 | 205篇 |
2012年 | 310篇 |
2011年 | 371篇 |
2010年 | 187篇 |
2009年 | 168篇 |
2008年 | 296篇 |
2007年 | 379篇 |
2006年 | 343篇 |
2005年 | 313篇 |
2004年 | 298篇 |
2003年 | 265篇 |
2002年 | 259篇 |
2001年 | 215篇 |
2000年 | 212篇 |
1999年 | 201篇 |
1998年 | 74篇 |
1997年 | 48篇 |
1996年 | 45篇 |
1995年 | 41篇 |
1994年 | 59篇 |
1993年 | 43篇 |
1992年 | 146篇 |
1991年 | 135篇 |
1990年 | 102篇 |
1989年 | 103篇 |
1988年 | 96篇 |
1987年 | 93篇 |
1986年 | 103篇 |
1985年 | 82篇 |
1984年 | 53篇 |
1983年 | 52篇 |
1979年 | 29篇 |
1977年 | 42篇 |
1974年 | 22篇 |
1973年 | 30篇 |
1972年 | 26篇 |
1971年 | 30篇 |
1970年 | 33篇 |
1969年 | 47篇 |
1968年 | 31篇 |
排序方式: 共有6876条查询结果,搜索用时 0 毫秒
91.
Inhibition of autophagy by chloroquine makes chemotherapy in nasopharyngeal carcinoma more efficient
Tomomi Aga Kazuhira Endo Akira Tsuji Mitsuharu Aga Makiko Moriyama-Kita Takayoshi Ueno Yosuke Nakanishi Miyako Hatano Satoru Kondo Hisashi Sugimoto Naohiro Wakisaka Tomokazu Yoshizaki 《Auris, nasus, larynx》2019,46(3):443-450
Objectives
A combination of platinum-based chemotherapy and radiotherapy is the standard treatment for nasopharyngeal carcinoma (NPC). However, the efficacy of chemotherapy has reached a plateau. Many autophagy studies suggest that autophagy can either promote or suppress to cancer progression. Thus, a role of autophagy in the acquisition of chemoradioresistance has recently been a notable event. Therefore, we examined the relationship between autophagy and chemotherapy in NPC.Methods
The expression of Beclin 1 and microtubule-associated protein light chain 3 (LC3), a marker of autophagy, was determined by immunohistochemistry in the biopsy samples of patients with NPC before and after the first course of chemotherapy. Additionally, to investigate in the effect of autophagy suppression in chemotherapy, NPC cell line C666-1 cells were treated with cisplatin and/or chloroquine, an inhibitor of autophagy.Results
The expression of Beclin 1 increased after chemotherapy in all patients. In NPC cell line C666-1, compared to cisplatin alone, combination therapy (cisplatin and chloroquine) reduced cell viability, and promoted cell apoptosis.Conclusions
These results suggest that autophagy, represented by Beclin 1, is upregulated after chemotherapy in both in vitro and in vivo NPC studies. Inhibition of autophagy could therefore be new strategy for NPC treatment. 相似文献92.
Effects of visible-light irradiation on vitreous structure in the presence of a photosensitizer 总被引:2,自引:0,他引:2
Sensitized photo-induced changes of vitreous structure were investigated using both in vivo and in vitro model systems. In the former, rabbit eyes were injected with the photosensitizer riboflavin, and in the latter, calf vitreous samples were treated with riboflavin or Methylene Blue prior to irradiation with white light. The active species of oxygen, i.e. singlet oxygen, superoxide anion, hydroxyl radical and hydrogen peroxide, generated by the photodynamic action of the sensitizer, caused significant liquefaction of the calf vitreous in vitro. There was little liquefaction of the rabbit vitreous in vivo, suggesting the presence of a protective mechanism in vivo. hyaluronidase induced significantly greater liquefaction in vitro than either Methylene Blue or riboflavin. This study suggests that loss of gel vitreous structure can result from extensive depolymerization of hyaluronidase by hyaluronidase and less drastic conformation and molecular weight changes in the photosensitized reactions. Although light-induced liquefaction was less marked than enzyme-induced liquefaction, the mechanism of the former is more pertinent to age-related vitreous synchysis. 相似文献
93.
94.
Hirotomo Dochi Satoru Kondo Takayuki Murata Masaki Fukuyo Asuka Nanbo Kousho Wakae WenPing Jiang Toshihide HamabeHoriike Mariko Tanaka Takumi Nishiuchi Harue Mizokami Makiko MoriyamaKita Eiji Kobayashi Nobuyuki Hirai Takeshi Komori Takayoshi Ueno Yosuke Nakanishi Miyako Hatano Kazuhira Endo Hisashi Sugimoto Naohiro Wakisaka ShinHun Juang Masamichi Muramatsu Atsushi Kaneda Tomokazu Yoshizaki 《Cancer science》2022,113(8):2862
95.
96.
Mai Kikumoto Shuichiro Neshige Takeo Shishido Hiroki Ueno Shiro Aoki Koji Iida Hirofumi Maruyama 《Internal medicine (Tokyo, Japan)》2022,61(8):1237
We evaluated a 39-year-old pregnant woman with right temporal lobe epilepsy. During the second trimester, seizure deterioration was responsive to an increased daily dose of levetiracetam (LEV). However, immediately after delivery, new non-habitual seizures emerged along with a sharply increased LEV concentration. The frequency of habitual seizures also slightly increased. The non-habitual seizures completely disappeared, and the frequency of the habitual seizures improved to the baseline level after the LEV dosage was reduced. Thus, a paradoxical effect of an increased LEV blood concentration was assumed to be a potential cause of these events. Peripartum pharmacokinetic fluctuations in LEV levels should be monitored carefully. 相似文献
97.
Hideto Kajitani Hiroki Nishiwaki Toshiharu Ueno Fumihiko Koiwa Shigeki Iwasaki Satoshi Hirade 《Internal medicine (Tokyo, Japan)》2022,61(8):1201
A 64-year-old Japanese man who worked at a butcher shop was hospitalized for a fever, headache, and deafness. We diagnosed him with sepsis and meningitis caused by Streptococcus suis infection. The patient''s renal function declined rapidly, and hemodialysis was performed temporarily. A renal biopsy was performed, and the renal function tended to improve with antimicrobial therapy. This case seemed rather similar to one of staphylococcal-associated nephritis in that it showed mesangial proliferative nephritis with immunoglobulin A deposition, even though the nephritis was caused by streptococci. Similarly, intramembranous electron-dense deposits were characteristic findings. We present new findings of an in vivo renal biopsy in a case of S. suis-associated glomerulonephritis. 相似文献
98.
99.
100.
Y. Ueno 《International journal of radiation biology》2013,89(4):307-312
SummaryWhen mice are maintained on a light-dark cycle in which the light begins at 06.00 and ends at 18.00 hours, endogenous spleen colony-forming cells are most radiosensitive at 02.00 and most radioresistant at 22.00 hours. There are three cycles of radiosensitivity with a cycle time of 8 hours. The results are discussed on the basis of the cellular kinetics of the haemopoietic cell system. 相似文献