癫痫患儿球结膜微循环的观察

<正> 癫痫是一种突然发生的短暂脑功能异常,且反复发作的神经症状,为小儿神经内科门诊的主要治疗对象。本文对我院30例癫痫患儿进行了球结膜微循环观察,发现大部分患儿有不同程度的微循环异常,与正常组对照有显著差异,现报导如下:     

肝素抑制后发性白内障形成的实验研究

肝素抑制后发性白内障形成的实验研究上海第二医科大学新华医院眼科夏小平，陆道炎，王丽天，陈才根，吴念祖目前有关减少后发性白内障形成的方法涉及二个方面。手术方面包括：尽可能多地清除晶体皮质和前囊膜上皮细胞、术中冷冻后囊、现在中山医科大学第三临床学院眼科尽...     

腹腔镜下胆囊和卵巢良性囊肿联合切除8例分析

目的探讨电视腹腔镜下联合切除胆囊及卵巢囊肿的可行性及优势。方法回顾分析了8例患者的手术过程及治疗结果。结果8例患者均一次完成胆囊及卵巢囊肿切除术,无一例中转开腹。手术时间平均为105min,胆囊切除术平均为45min,卵巢囊肿切除平均为60min。住院日平均为6d。结论腹腔镜下联合切除胆囊、卵巢囊肿不增加手术的危险性及并发症,同时完成两种手术减少了患者的痛苦和手术费用。医患双方均满意。     

ACTIVIT OF HUMAN PLACENTAL GAMMA GLOBULININ BLOCKING IMMUNE FUNCTIONS IN VITRO AND IN ABROGATING THE XENOGENEIC, LOCAL GRAFT-VERSUS-HOST-REEACTEON

In order to understand the mechanism of immunosuppression caused by infusion of placental gamma globulin (PGG) in patients with renal allografts, rheumatoid arthritis, and graft – versus –host disease (GCHD) following bone marrow transplantation (BMT) ,we have examined the effect of PGG in vitro and in a model of the xenogeneic , local graft –ver- sus – host reaction (LGVHR) .PGG inhibited lymphocyte proliferation in mixed lymphocyte cultures (MLC) (P<0.005) and depressed interleukin -2 (IL-2) levels in such cultures at 72 hours (P<0.01) . In contrast phytohemagglutinin (PHA) –and pokeweed mitogen (PWM) –induced T and B lymphocyte blastogenesis was not affected by such PGG treatment .PGG treatment .PGG neither decreased the [3H] TdR pulse incorporation in unstimulated lymphocytes nor affected cell viability .Cell cycle analysis by flow cytometry showed that PGG reduced the percentage of cells in S and G2, M phases during the MLC, but did not alter cell cycling during PWM-stimulated proliferation . An immunosuppressive effect of PGG on the LGCHR was tested in a model of intracutaneous transplantation of PGG –treat human lymphocytes into cyclophosphamide – immunosuppressed rats. Lymphoprep – separated human tonsillar lymphocytes were incubated with RPMI-1640 buffer containing：（1）PGG,4mg/ml,(2) human plasma albumin,4mg/ml,(3)mitomycin-C,25ug/ml, or (4) no additive. Cell of each preparation (3x107cells in 0.1ml) were injected intracutaneously into cyclophosphamide-treated male rats at separate abdominal locations. A fifth site received only the buffer solution. Five days after injection of cells ,each rat received [125 I]IUdR (10uCi) intraperitoneally and was killed after 5 hours. For each site of injection, the diameters of induration were measured and 125 I was counted . There was no difference between buffer – treated and a ibumin – treated groups either in the diameter of the area of induration (t=0.66;P>0.5)or in radioactive counts(t=0.22;P>0.05).In the PGG –treated group, the induration and radioactivity measurements were significantly less than in control groups (t=3.72 and P<0.1;t=2.62 and P<0.02,respectively ) Cytophilic antibodies in PGG were thought to inhibit an early phase of T cell activation, and not to be cytotoxicity .In the LGVHR, the immune response might be abrogated either by immuno- regulatory suppression of T cell function or by toxicity to the infused lymphoid cells. For some clinical purposes, immuno- modulating, human antibodies might be preferred to murine, monoclonal antibodies.     

软性人工晶体和白内障超声乳化小切口植入

作者最近应世界人工晶体基金会的邀请,在美国作了短期参观学习。在此期间,通过交谈、直接观看手术和录像以及近期的文献资料,得到一个深刻的印象,即软性人工晶体作为一种新材料、新类型和新方法正在兴起。软性人工晶体可折叠起来,通过超声乳化术3mm的小切口植入眼内,伤口只需缝合一针。更新的研究表明,今后有     

原位杂交与免疫组化技术在HPV检测中的应用

目的：对两种HPV检测技术DNA原位杂交和免疫组化染色方法进行对比观察。方法：选取本院外阴尖锐湿疣病例95例，组织经固定、脱水、石蜡包埋、切片，分别采用原位杂交和免疫组化技术进行检测。结果：HPV—DNA阳性率为94．7％(90／95)，阳性反应由鳞状上皮表层的空泡细胞核内延伸至棘层中下部近基底层的细胞核内；HPV—Ag阳性率为63．1％(60／95)，阳性反应主要位于鳞状上皮表层的空泡细胞核内及角质层内。个别阳性细胞可在棘层出现。结论：原位杂交技术在敏感度、阳性率及阳性强度、背景染色等方面均优于免疫组化方法，为尖锐湿疣的确诊及临床治疗提供更准确、可靠的依据。     

中南地区正常人血清α_1─抗胰蛋白酶检测结果分析

通过对中南地区１５７７例正常人的血清α_１－抗胰蛋白酶（α_１－ＡＴ）水平的测定，结果２０～４０岁组正常值为１３６．９ｍｇ％，４１～６０岁组正常值为１２９．３ｍｇ％，男女性别间的α_１－ＡＴ水平差异无显著意义。而两个年龄组间差异有显著意义。并对测定结果作了分型。     

我国司法精神病学的历史与发展

司法精神病学是一门边缘学科,既是精神病学的一个分支,又是法医学的一个分支,这种双重属性不仅决定了它在学科上的特殊地位,而且由于我国特有的司法精神病学鉴定机构设置,使得管理体制显得十分复杂,许多问题至今仍未完全解决。在全国人大《关于司法鉴定管理问题的决定》(2005)(