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排序方式: 共有268条查询结果,搜索用时 93 毫秒
71.
Recombinant human factor IX: replacement therapy, prophylaxis, and pharmacokinetics in canine hemophilia B 总被引:1,自引:2,他引:1
Brinkhous KM; Sigman JL; Read MS; Stewart PF; McCarthy KP; Timony GA; Leppanen SD; Rup BJ; Keith JC Jr; Garzone PD; Schaub RG 《Blood》1996,88(7):2603-2610
Recombinant human factor IX (rFIX) has been expressed in transduced cultured cell systems since 1985. Because there has been limited in vivo testing of rFIX in hemophilia B subjects, this study was undertaken using the severe hemophilia B canines of the Chapel Hill strain. Three groups of hemophilic dogs received either 50, 100, or 200 IU/kg of rFIX. As a control, a fourth group of hemophilic dogs received 50 IU/kg of a high purity, plasma-derived human FIX (pdFIX). The coagulant and hemostatic effects of rFIX and pdFIX were similar with all comparative dosing regimens. Based on activity data, the elimination half-life of rFIX was 18.9 +/- 2.3 hours and pdFIX was 17.9 +/- 2.1 hours. A prophylactic regimen administering rFIX daily resulted in a continuous therapeutic level of plasma FIX and was accompanied by a two-fold increase in recovery levels by day 5, compared to that observed with administration of a single bolus. The mechanisms of the high to complete recovery of FIX with the prophylactic regimen could depend not only on the degree of saturation of the vascular endothelial binding sites but also on the altered dynamics of the balance of FIX distribution between the intravascular and extravascular compartments. The pharmacokinetic (PK) parameters for rFIX and pdFIX were similar. However, the relative PK values for V1 and V5s of both products on day 5 differed greatly from day 1 and may reflect the changing equilibrium of FIX between compartments with elevated levels of plasma FIX. Neutralizing antihuman FIX antibodies resulting from human FIX antigen being administered to FIX deficient dogs were observed beginning at 14 days. The antigenicity of rFIX and pdFIX appeared to be comparable. Despite the very different procedures used for production of rFIX and pdFIX products, in vivo testing in hemophilia B dogs showed the functional behavior of these products is similar; they are highly effective for replacement therapy and for prophylaxis. 相似文献
72.
Anti-My-28, an antigranulocyte mouse monoclonal antibody, binds to a sugar sequence in lacto-N-neotetraose 总被引:5,自引:0,他引:5
Spitalnik SL; Schwartz JF; Magnani JL; Roberts DD; Spitalnik PF; Civin CI; Ginsburg V 《Blood》1985,66(2):319-326
Anti-My-28 is an IgM kappa monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocytes and lymphocytes. However, after treating cells with neuraminidase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My- 28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Gal beta 1-4GlcNAc beta 1-3Gal beta 1- 4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins. 相似文献
73.
Karen L Andrews Xiao L Moore Jaye PF Chin‐Dusting 《Clinical and experimental pharmacology & physiology》2010,37(7):736-742
1. The endothelium is critical in the control of vascular haemodynamics and haemostasis. Endothelial dysfunction, typically characterized by decreased nitric oxide bioavailability and response to endothelium‐dependent agonists, is well accepted as a defining characteristic of early atherosclerosis. 2. Numerous epidemiological studies have reported that increased levels of circulating HDL are vasculoprotective and reduce the incidence of adverse cardiovascular events. Traditionally, these effects have been attributed to the ability of HDL to remove cholesterol from cells via reverse cholesterol transport. However, there is increasing evidence that the beneficial effects on the endothelium by HDL encompass its anti‐inflammatory, antithrombotic and anti‐oxidative properties, which include the release of nitric oxide (NO). 3. This review highlights recent findings on the importance of HDL in reducing atherosclerotic risk. We focus on the beneficial effects of HDL‐induced NO release and how this relates to endothelial dysfunction and on the effect of HDL on vascular repair via endothelial progenitor cells. 相似文献
74.
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76.
Vegetti W; Grazia Tibiletti M; Testa G; de Lauretis Yankowski; Alagna F; Castoldi E; Taborelli M; Motta T; Bolis PF; Dalpra L; Crosignani PG 《Human reproduction (Oxford, England)》1998,13(7):1796-1800
Premature ovarian failure is defined as cessation of ovarian function under
the age of 40 years and affects approximately 1% of women in the general
population. The aetiology of this disorder is still unknown in most cases.
Although there have been some reports of familial premature ovarian
failure, very little is known about the incidence and inheritance pattern
of its idiopathic form. The aims of this study were to investigate the
incidence and inheritance pattern of familial premature ovarian failure in
a homogeneous group of patients with premature idiopathic menopause and to
identify possible clinical differences between patients with the familial
and the sporadic form of premature ovarian failure. A total of 71 women
were recruited into the study. Clinical assessments and genetic counselling
showed that 22 (31%) patients had familial premature ovarian failure, this
high incidence strongly suggesting that the disorder is a recognizable
heritable entity. There was a statistically significant (P < 0.05)
difference in the median age of precocious menopause in patients with
sporadic and familial premature ovarian failure (31.0 and 37.5 years of age
in the two groups, respectively). Pedigree analysis strongly suggests the
existence of a familial pattern of premature ovarian failure with a
dominant maternal and/or paternal transmission and incomplete penetrance.
In the presence of familial history of premature ovarian failure,
reproductive counselling is recommended.
相似文献
77.
78.
Miraldi FD; Nelson AD; Kraly C; Ellery S; Landmeier B; Coccia PF; Strandjord SE; Cheung NK 《Radiology》1986,161(2):413-418
In a previous study, the authors showed that iodine-131 labeled monoclonal antibody (Mab 3F8) could be used to image human neuroblastoma xenografts in mice with excellent tumor-to-tissue ratios. In this study they report their experience with six patients scanned with radiolabeled 3F8. There was strong accumulation of the labeled antibody in viable tumor, but no significant uptake was noted in normal brain, liver, spleen, or adrenal glands. Tumor-to-nontumor activity ratios varied but were approximately 10:1-20:1. This ratio yields good contrast for visualization. Time-activity curves show that radioactivity levels in normal tissue have a half-time of about 40 hours, whereas tumor tissues show a half-time of about 60 hours. Significant gastric secretion of free iodine demonstrated that the Mab was being deiodinated. Calculated radiation doses indicate that tumors receive at least ten times the dose to other tissues. The results indicate that Mab 3F8 has clinical potential for both imaging and therapy of human neuroblastomas. 相似文献
79.
Montgomery Rice V; Limback SD; Roby KF; Terranova PF 《Human reproduction (Oxford, England)》1998,13(5):1285-1291
This study determined effects of follicle stimulating hormone (FSH) alone
and in combination with tumour necrosis factor (TNF), on granulosa cells
from small (5-10 mm diameter) and large (>10-25 mm) follicles during
follicular and luteal phases of the cycle and during periods of acyclicity.
Granulosa cells were collected from ovaries of premenopausal women
undergoing oophorectomy. The cells were cultured with human FSH (2 ng/ml)
and testosterone (1 microM) in the presence or absence of human TNF-alpha
(20 ng/ml). Media were removed at 48 and 96 h after culture and
progesterone, oestradiol and cAMP in media were measured by
radioimmunoassays. FSH stimulated the accumulation of oestradiol from
granulosa cells of small follicles during the follicular and luteal phases
but not during acyclicity; and TNF reduced oestradiol accumulation in the
presence of FSH. Interestingly, in granulosa cells from small follicles,
progesterone and cAMP secretion increased in response to FSH and neither
was affected by TNF. Thus, TNF specifically inhibited the conversion of
testosterone to oestradiol in granulosa cells from small follicles. FSH
stimulated oestradiol production by granulosa cells of large follicles
obtained only during the follicular phase of the cycle and TNF inhibited
the FSH-induced oestradiol secretion. Granulosa cells obtained from large
follicles during the luteal phase and during acyclicity did not accumulate
oestradiol in response to FSH. However, FSH increased progesterone and cAMP
secretion by granulosa cells obtained from large follicles during the
follicular and luteal phases. During the luteal phase alone, TNF in
combination with FSH increased progesterone accumulation above that of FSH
alone. FSH did not increase progesterone, oestradiol or cAMP secretion by
granulosa cells obtained from large follicles during acyclicity. Thus, FSH
increases progesterone, oestradiol and cAMP secretion by granulosa cells of
small follicles during the follicular and luteal phases and TNF appears to
inhibit FSH-induced oestradiol secretion specifically in those cells. In
large follicles, FSH- stimulated granulosa cell secretion of oestradiol is
limited to the follicular phase and this effect can be inhibited by TNF. In
addition, when granulosa cells of large follicles do not increase
oestradiol secretion in response to FSH, TNF stimulates progesterone
secretion.
相似文献
80.
Anemone?van den BergEmail author Ruurd?M?van Elburg Jos?WR?Twisk Willem?PF?Fetter 《BMC pediatrics》2004,4(1):17