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Jiagang Tu Wenjie Chen Xiaozhe Fu Qiang Lin Ouqin Chang Lijuan Zhao Jiangfeng Lan Ningqiu Li Li Lin 《International journal of molecular sciences》2016,17(5)
Nervous necrosis virus (NNV) is the causative agent of viral encephalopathy and retinopathy (VER), a neurological disease responsible for high mortality of fish species worldwide. Taking advantage of our established Chinese perch brain (CPB) cell line derived from brain tissues of Mandarin fish (Siniperca chuatsi), the susceptibility of CPB cell to Red-Spotted Grouper nervous necrosis virus (RGNNV) was evaluated. The results showed that RGNNV replicated well in CPB cells, resulting in cellular apoptosis. Moreover, the susceptibility of Mandarin fish to RGNNV was also evaluated. Abnormal swimming was observed in RGNNV-infected Mandarin fish. In addition, the cellular vacuolation and viral particles were also observed in brain tissues of RGNNV-infected Mandarin fish by Hematoxylin-eosin staining or electronic microscopy. The established RGNNV susceptible brain cell line from freshwater fish will pave a new way for the study of the pathogenicity and replication of NNV in the future. 相似文献
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Xing Fan Shou‐Ze Wang Ai‐Li Zheng Xian‐Yong Wei Yun‐Peng Zhao Zhi‐Min Zong Wei Zhao 《Microscopy research and technique》2013,76(7):744-750
Infrared light from a pulsed optical parametric oscillator laser system was used to irradiate succinic acid (SA), a usual solid matrix used in matrix‐assisted laser desorption ionization, under vacuum. Ablated SA particles were trapped using a silica plate mounted 3.0 mm above and parallel to the sample surface. The morphology and particle size of ablated particles at different laser fluences were investigated using a scanning electron microscope (SEM). The dynamics of plume propagation for SA desorption process was studied with fast photography at atmospheric pressure. Plume expanding at 1.12 J/cm2 laser fluence was recorded using a high‐speed CMOS camera and corresponding propagation distance was measured. The solid matrix desorption was driven by phase explosion according to plume model fitting, which was consistent with the results of SEM. Microsc. Res. Tech. 76:744–750, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
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土石混合体作为土体和碎石的混合体,其宏观力学特性与碎石特性密切相关。采用试验研究和数值模拟相结合的方法,对土石混合体的力学特性进行研究。基于FISH语言开发颗粒流的多边形碎石生成模块,通过室内试验校核颗粒流模型的细观参数,建立土石混合体双轴试验模型。提出以形状因子m作为衡量碎石磨圆度的指标,对碎石形状进行了量化。研究了碎石含量、强度、形状对土石混合体力学特性影响,探讨碎石对土体强度提高的细观原因,揭示土石混合料的强度指标随各主要影响因素的变化趋势。结果表明:碎石含量和强度越高,混合体的强度也越高,当含石量小于40%时,土石混合体的力学特性受土体主导;内摩擦角随着碎石含量增加而增大,而黏聚力却在减小;随着形状因子的增大,强度降低,黏聚力和内摩擦角均减小;土石混合体中应力分布不均,在碎石含量较高时,碎石形成骨架效应,承担了主要荷载。 相似文献
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Zhen‐Zhong Huang Xue‐Liang Pei Ting Wu Shou‐Ri Sheng Shu‐Ying Lin Cai‐Sheng Song 《应用聚合物科学杂志》2011,119(2):702-708
A series of novel fluorinated aromatic polyesters containing trifluoromethylphenoxy pendant groups was synthesized by interfacial polycondensation of 2‐(4‐trifluoromethylphenoxy)terephthalyl chloride with various bisphenols in dichloromethane. The polyesters obtained in good yields had weight‐average molecular weights of 70,600–29,800 g/mol, polydispersities of 1.81–2.08, and were all amorphous. All polyesters were easily soluble in organic solvents such as N,N‐dimethylformamide, tetrahydrofuran, o‐chlorophenol, pyridine, and dichloromethane. These fluorinated polyesters showed glass transition temperature of 133–210°C, and good thermal stability with almost no weight loss up to 378°C, the 10% weight loss temperature of 472–523°C as well as char yield of 32–63% at 600°C in nitrogen. These polyester films cast from chloroform solutions exhibited tensile strengths ranging from 102 to 126 MPa, elongation at break from 6.3% to 11.7%, and tensile moduli from 2.1 to 3.3 GPa. The resulting polyester films also displayed low dielectric constants between 2.18 and 2.49 (1 MHz), high transparency with an ultraviolet‐visible absorption cut‐off wavelengths in the 332–355 nm range, and excellent electric strengths (50.4–65.6 kV/mm) and volume resistivity (2.51–6.03 × 1016 Ω cm). © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献
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