含环碳酸酯基新型聚合物载体的合成及固定化葡萄糖淀粉酶研究

碳酸亚乙烯酯(VCA)分子中的反应性环碳酸酯基可于温和条件下与氨基发生反应形成稳定的氨基甲酸酯.利用这一性质,将含-NH₂基团的酶分子直接以σ键的形式固定于含有环碳酸酯基的聚合物载体上.本文通过反相悬浮聚合,以液体石蜡为介质,VCA为反应性单体,甲基丙烯酸-β-羟乙酯(HEMA)及丙烯酸羟丙酯(HPA)为亲水性共聚单体,合成出一系列交联树脂聚合物.以此聚合物为载体对葡萄糖淀粉酶进行固载实验,表现出良好的固定化性能.同时,固定化酶的稳定性也有所提高.     

核果类水果干物质含量近红外无损检测研究

以桃、李及杏果实为材料,研究其干物质含量的近红外漫反射无损检测模型的建立方法。研究发现,桃、李及杏果实干物质预测模型的决定系数(r_P~2)分别为0.901、0.909及0.923,预测均方根误差(RMSEP)分别为0.365、0.401及0.465,相对分析误差(RPD_P)分别达2.9、3.2及3.1以上。结果表明,近红外漫反射光谱可用于准确、快速、无损定量3种水果中干物质含量。     

蛋白质组学及其在重离子治癌应用中的探讨

综述了蛋白质组学的主要方法和技术——双向凝胶电泳、生物质谱技术、蛋白质芯片技术，及其在肿瘤标志物的筛选和鉴定、肿瘤治疗效果的评价及肿瘤发生机制研究等方面的应用状况，并对蛋白质组学在重离子治疗中的应用进行了展望。With the accomplishment of the human genome project, proteomics becomes a new snbject on the buildup of the whole proteins and their dynamic changes in cell emerges. Cancer is a kind of complex disease involved in multi-genes and proteins. Heavy ion therapy is all arising and potential radiation treatment nowadays. This paper reviews on the main methods and technology in proteomics-- two-dimensional gel electrophoresis （2-DE）,biological mass spectrometry, protein biochips, bioinformatics and its application on identification of cancer biomarkers, evaluation of curative effect on tumour and the mechanisms of turnout formation. This paper also presents some prospects on the application of proteomics in heavv ion therapy.     

RELATIONSHIPS BETWEEN IONIZING RADIATION,BONE MARROW TRANSPLANTATION AND LEUKEMOGENESIS

Studies of the relationships between ionizing radiation, bone marrow transplantation and leukemo-genesis were carried out in 343 LACA mice. The recipients (female) were given whole-body irradiationwith 7 or 8 Cy of Co-60 γ-rays, while the donors (male) were given whole-body irradiation with 3, 1.5,0.5, 0.1, 0.05 and 0 Gy of Co-60 γ-rays. Each recipient was intravenously infused with 1-3 ×10~7 of themixed marrow cells of donors. In the result, the average incidence of surviving over 1 month was 86% inthe recipients. Myelocytic leukemia developed in all the transplanted groups, the average incidence being88.5%. Leukemia was observed in 1.5-2.5 months after transplantation in the recipients receiving marrowcells from donors exposed to 3 Gy, but in 5-8 months in other groups. It was demonstrated by the analysis of Y-chromosome that the leukemic cells were derived from the donor's marrow cells. The resultssuggest that marrow transplantation for the mice irradiated by Co-60 γ-rays of lethal dose can prote     

Characteristics Analysis of Vertical Double Gate Strained Channel Heterostructure Metal-Oxide-Semiconductor-Field-Effect-Transistor

Since device feature size shrinks continuously, there appears various short-channel effects on the fabrication and performance of devices and integrated circuits. We present a vertical double gate （VDG） strained channel heterostrueture metal-oxide-semiconduetor-field-effect-transistor （MOSFET）. The electrical characteristics of the device with the effective gate length scaled down to 60nm are simulated. The results show that the drive current and transconductance are improved by 57.92% and 54.53% respectively, and grid swing is decreased by 36.83% over their unstrained counterparts. VDG MOSFETs exhibit a stronger capability to restrict short-channel-effects over traditional MOSFETs.     

基于物理原理的声源定位实验设计

本文使用信号发生器、直流稳压电源、数字示波器、直流电机、四元麦克风线阵、数显角度尺、扬声器等常规物理实验仪器和部分自制装置搭建了一个室内声源定位系统,利用直流电机带动四元麦克风线阵和数显角度尺同轴旋转,将四元麦克风线阵接收到的声波信号经放大和滤波处理后输入示波器,根据示波器上利萨如图形的形态判断声源方位,由数显角度尺测量线阵的转动角,再利用三角函数计算出声源的位置坐标.实验结果表明,此方法进行声源定位精度高,误差小,成本低.