bFGF促增殖后骨髓间充质干细胞向多巴胺能神经元样细胞的分化

目的探讨碱性成纤维细胞生长因子(Basic fibroblast growth factor,bFGF)体外作用于骨髓间充质干细胞(Mesen-chymal stem cells,MSCs)后,诱导其向神经元样细胞和多巴胺能神经元样细胞定向分化的情况。方法从鼠骨髓中获得MSCs,培养传代,用MTT法检测bFGF对骨髓MSCs生长的影响。10 ng/ml bFGF作用2 d后,通过IBMX、细胞因子bFGF、GDNFI、L-1β、中脑神经胶质细胞条件培养基和中脑神经细胞膜碎片等分组联合诱导骨髓MSCs向神经元样细胞、多巴胺能神经元样细胞分化,免疫细胞化学方法鉴定特异标志NSE、MAP-2a,b和TH的表达。结果在一定范围内,bFGF对骨髓MSCs具有明显的促增殖作用。分化的神经元样细胞表达NSE、MAP-2a,b和TH,联合应用GDNFI、L-1β、中脑条件培养基和中脑神经细胞膜碎片诱导7 d后,NSE阳性率为(27.774±2.747)%,MAP-2a,b为(28.006±3.080)%,TH为(3.098±0.352)%。结论体外骨髓MSCs被诱导分化成神经元样细胞和多巴胺能神经元样细胞,为帕金森等中枢神经系统疾病的细胞移植治疗奠定了基础。     

西气东输三线伊宁-霍尔果斯煤制气支线工程水土保持监测及分析

西气东输三线伊宁-霍尔果斯煤制气支线工程的水土保持监测过程及结果,全面反映了工程建设沿线的水土流失情况,以及工程建设中所引起水土流失的区域,为以后同类型工程建设中水土保持设计和水土保持监测重点监测范围提供了一定的设计及监测依据。     

双碳源在LiFePO4/C微波固相合成中的协同效应

以Li2CO3,FeC2O4·2H2O和NH4H2PO4为前驱体,分别以葡萄糖和葡萄糖/乙炔黑为碳源,利用微波加热合成了LiFePO4/C正极材料.用X射线粉末衍射(XRD)和扫描电镜(SEM)对材料进行了表征,用四探针法测定了材料的电导率.研究了碳源与微波温度对材料微结构和电化学性能的影响,发现由于乙炔黑的协同效应,用双碳源在600℃反应即可得到最佳电化学性能的LiFePO4/C,而仅用葡萄糖作碳源反应需要在较高温度(如700℃)下进行.     

Thermo-plasticity of high-strength and high-ductility 7050 aluminum ingot

Thermo-plasticity of homogenized 7050 aluminum ingot was investigated by instantaneous tensile tests conducted at different temperatures. The results show that, with the increase of testing temperatures, the strength decreases, and the plasticity increases firstly and then decreases in homogenized 7050 ingot. When the studied alloy is deformed between 380 °C and 420 °C, the deformation resistance is lower and plasticity is better. And the actual heating temperature for ingot before hot extrusion should be controlled between 360 °C and 400 °C. At low tensile temperatures, the deformation structure is mainly composed of dislocation substructure. With the increase of testing temperatures, transgranular fracture transforms into intergranular fracture progressively during deformation. At high tensile temperatures, the grain boundaries are weakened, deformation is concentrated at the grain boundaries and the re-orientation of equilibrium phases at grain boundaries appears.     

困境中求生存 变化中谋发展

移民开发中心(以下简称中心)是松辽委直属差额事业单位,成立于1990年,现有编制15人。20多年来,中心曾经有过辉煌,也曾陷于低谷,但是不论处于什么样的环境,中心的全体同志都能按照委党组的部署,团结一致,克服困难,努力工作,圆满完成了各项工作任务。现将22年的工作做以     

无限深土石坝坝基悬挂式垂直防渗体防渗方法初探

筑坝工艺的提高和计算分析手段的发展,解决了在无限深透水地基上建坝所面临的多方面问题,但在某些方面,仍需改进和完善。通过理论计算和物理模型试验,研究和探讨了采用悬挂式垂直防渗体解决水库渗漏的问题,对在无限深透水地基上建坝的问题进行了分析探讨。     

Hot compressive deformation behavior and constitutive relationship of Al-Zn-Mg-Zr alloy with trace amounts of Sc

Abstract： The hot deformation behaviors of AI-Zn-Mg-Sc-Zr alloy were investigated in a temperature range of 340-500℃ and a strain rate range of 0.001-10 s 1 using uniaxial compression test on Gleeble-1500 thermal simulation machine. The results show that the flow stress increases with increasing strain and tends to be constant after a peak value. The flow stress increases with increasing strain rate and decreases with increasing deformation temperature. The phenomenon of dynamic recovery and dynamic recrystallization can be observed by microstructural evolutions. Based on the hyperbolic Arrhenius-type equation, the true stress-true strain data from the tests were employed to establish the constitutive equation considering the effect of the true strain on material constants （α, β, Q, n and A）, which reveals the dependence of the flow stress on strain, strain rate and deformation temperature. The predicted stress-strain curves are in good agreement with experimental results, which confirms that the developed constitutive equations are suitable to research the hot deformation behaviors of Al-Zn-Mg-Sc-Zr alloy.     

内河航道采砂监测系统设计及应用

针对违法违规采砂活动严重影响采砂河段河床的自然变化,对采砂河段的防洪安全、船舶航行安全及生态环境等都带来不良影响的问题,在查阅国内外大量文献的基础上,分析了国内外采砂监管技术发展现状、重点河道的采砂监管现状、长江航道监控信息化技术现状,研究了采砂区域、采砂量、采砂区域航道结构信号的自动采集和识别方法,给出了长江航道采砂实时监测系统设计方案。实际应用表明,根据该设计方案和关键处理技术研制的长江航道采砂远程监控系统应用效果良好,取得了较好的社会和经济效益,对提高内河航道服务品质及内河航道通行能力起到了重要的促进作用。     

等离子体活化烧结法制备多孔钛研究

采用等离子体活化烧结技术制得孔径大小可调、贯通性良好的多孔钛,确立了使用等离子体活化烧结法制备多孔钛的工艺流程,并确定了相关工艺参数,研究了发泡剂添加量和试块成型压力对多孔钛孔隙特征的影响,观察了多孔钛的显微孔隙特征.