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Introduction
The aim of this study was to determine the influence of the far upstream element binding protein 1 gene (FUBP1) on chemotherapy sensitivity in human U251 glioblastoma cells.Material and methods
Real-time polymerase chain reaction (PCR) was used to determine the expression of the FUBP1 gene in 43 cases of human brain gliomas. Western blot analysis was used to determine the inhibitory effect of RNA interference on FUBP1 gene expression. Methyl thiazolyl tetrazolium assay (MTT) and flow cytometry methods were used to determine the growth inhibitory rate and apoptosis rate of the U251 cells with FUBP1 silencing. The growth inhibitory rate and apoptosis rate were further determined after treatment of those U251 cells with cisplatin (DDP).Results
The expression of FUBP1 mRNA was up-regulated significantly in gliomas, 177.65% as much as in peri-cancerous tissues (p < 0.05). The expression of FUBP1 protein was inhibited significantly with siRNA-FUBP1 (p < 0.05). In FUBP1-silenced cells, the growth inhibitory rate increased from 1.4% to 29.5%, and the apoptosis rate increased from 2.68% to 5.84% (p < 0.05 for both). After treating with DDP at various concentrations (1, 3, 5 µg/ml), the growth inhibitory rate of FUBP1-silenced cells increased from 14.42%, 17.46% and 23.55% to 21.69%, 27.51% and 37.57%; the apoptosis rate increased from 8.85%, 14.37% and 18.21% to 13.25%, 18.46% and 26.52%.Conclusions
The up-regulation of FUBP1 relates to the carcinogenesis of gliomas. FUBP1 silencing increases the growth inhibitory rate and apoptosis rate of the U251 cells, and enhances the chemotherapy sensitivity of U251 cells to DDP. 相似文献Material and methods: The diameters of the splenic vein (SV), the left renal vein (LRV), and the vertical interval between them, were measured in computer tomography (CT) images obtained from 30 patients with portal hypertension and in 20 adult cadavers. The magnetic devices used for the splenorenal shunt were then manufactured based on the anatomic parameters measured above. The observation of the anatomical structure showed there were no special structural tissues or any important organs between SV and LRV. Then the magnetic compression splenorenal shunt procedure was performed in three dogs and five cadavers. Seven days later, the necrotic tissue between the two magnets was shed and the magnets were removed with the anchor wire.
Results: The feasibility of splenorenal shunt via MCT was successfully shown in both canine and cadaver, thus providing a theoretical support for future clinical application. 相似文献
Background
Ovarian serous carcinoma (OSC) is the most common ovarian epithelial malignancy. Disregulation of Eph/ephrin signaling has been implicated in oncogenesis and tumor progression. EphA5 receptor is one of large families of Eph tyrosine kinase receptor and is documented in the development of nervous system. Till now, there is no published data about the role of EphA5 in ovarian epithelial neoplasmas.Methods
This study aims to investigate the expression of EphA5 protein in ovarian serous carcinoma, and its relationship to clinical pathological characteristics. Sixty-one cases of ovarian serous carcinoma, 24 cases of benign ovarian serous tumors, 42 cases of serous borderline tumors and 20 cases of normal fallopian tubes were examined using immunohistochemical staining. The relationship between EphA5 expression and pathological parameters was analyzed. Kaplan-Meier survival function was used to analyze prognosis of patients.Results
Immunostaining analysis demonstrated that the EphA5 protein was highly expressed in 100% (20/20) of normal fallopian tube samples, 100% (24/24) of benign epithelial ovarian tumors, 76% (32/42) of ovarian serous borderline tumors, and 31% (19/61) of ovarian serous carcinomas. Loss of EphA5expression was associated with tumor grade (P?<?0.001) and FIGO stage (P?=?0.005). The survival analysis showed that patients with negative or weak expression of EphA5 protein had a poor outcome than those with positive expression (P?=?0.004).Conclusions
Our results show that EphA5 may be a potential biomarker for distinguishing high-and low-grade ovarian serous carcinoma and a potential prognostic marker.目的 探讨人类非嗜肝病毒所致肝炎的病因和临床特征。方法 对176例人类非嗜肝病毒性肝炎患者进行临床研究,用常规方法检测112例甲-戊型肝炎标志物,排除嗜肝病毒感染。检测单纯疱疹病毒(HSV)、EB病毒(EBV)、巨细胞病毒(CMV)、柯萨奇病毒(CoxV)等病毒的IgM、IgG型抗体和自身抗体(线粒体抗体和抗核抗体),随访6个月,并将其临床症状体征、肝功能指标与同期急性病毒性肝炎比较。结果 非嗜肝病毒肝炎患者病原体以CMV感染最多(34.7%),其次分别为EB病毒和轮状病毒感染(24.4%、9.6%),非嗜肝病毒肝炎患者的乏力、纳差、厌油、恶心、肝肿大、皮肤黄染发生率较同期急性病毒性肝炎低,两者比较差异有统计学意义(P <0.01),其脾肿大、淋巴结肿大的发生率较同期急性病毒性肝炎高,两者比较差异有统计学意义(P <0.01);非嗜肝病毒肝炎患者肝功能的ALT、AST、TBIL值较同期的急性病毒性肝炎值低,两者比较差异有统计学意义(P <0.01),清蛋白、凝血酶原时间值与急性病毒性肝炎比较,差异无统计学意义(P > 0.05);非嗜肝病毒肝炎患者的单一感染的ALT、AST、TBIL较复合感染时间短,两者比较差异有统计学意义(P <0.01)。结论 巨细胞病毒、EB病毒等为非嗜肝病毒肝炎的常见病原体,临床表现为急性肝损伤,但较急性病毒性肝炎轻,单一感染较复合感染轻,预后较好。
相似文献目的 Betatrophin是一种新发现的影响糖脂代谢的因子。糖尿病和高脂血症是冠状动脉粥样硬化性心脏病(以下简称冠心病)的重要危险因素。比较冠心病患者与对照组的Betatrophin水平,并探讨其与三酰甘油(TG)和糖化血红蛋白(HbA1c)等因素的相关性。方法 测定冠心病组(107例)及与其年龄、性别、体重指数匹配的对照组(35例)的Betatrophin水平。根据HbA1c水平将冠心病组分为冠心病HbA1c正常组和冠心病HbA1c升高组。获取其他临床指标,并测定TG、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)、高密度脂蛋白胆固醇(HDL)、肌酐(Scr)、尿酸(UA)、超敏C反应蛋白(hsCRP)、脑钠肽(BNP)和同型半胱氨酸(Hcy)水平等,分析Betatrophin与其的相关性。结果 冠心病组Betatrophin水平比对照组降低26.6%(P =0.040)。冠心病HbA1c正常组比对照组降低33.0%(P =0.001),冠心病HbA1c升高组比冠心病HbA1c正常组Betatrophin水平升高22.6%(P =0.020)。冠心病组患者Betatrophin与HbA1c呈正相关(r =0.223,P =0.021)。冠心病组男性患者比女性Betatrophin水平更低[(0.149±0.068) vs (0.181±0.078)ng/ml,P =0.028]。结论 Betatrophin可能是与冠心病患者糖脂代谢调节相关的一个新因子。
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