正交设计优化青蒿SRAP-PCR反应体系及引物筛选

为了建立青蒿的SRAP最佳扩增体系,并筛选出SRAP多态性引物,本研究以青蒿叶片DNA为模板,采用正交试验设计,以Mg^2+、dNTP Mix、Taq DNA聚合酶、引物和DNA模板5种因素5个水平,对青蒿SRAP反应体系进行研究。结果表明,青蒿SRAP-PCR最佳反应体系为:引物0.6μmol/L、Mg^2+2.0 mmol/L、模板DNA 5.1 ng、Taq DNA聚合酶2.0 U、dNTPs 0.25 mmol/L,总体积为25μL。各因素对扩增反应均有不同影响,其中引物浓度的影响最大,dNTPs的影响最小。运用该体系对不同种质资源的青蒿进行验证,证明该体系稳定可靠,并在30个引物组合中筛选出了25对扩增条带清晰,多态性丰富的引物组合。这一结论为今后利用SRAP标记技术进行青蒿分子遗传学研究提供了科学依据。     

基于遥感指数的草原火后植被恢复研究

草原火是草原生态系统重要的干扰因子，严重影响着系统的结构与功能。基于遥感数据，以2015年“4·16”特大草原火灾为例，利用ENVI和ArcGIS等软件，分别对NDVI和GPP指数及火烧严重度下的植被恢复过程进行定量分析。结果表明，基于NDVI和GPP指数的火后植被恢复过程表现相似，不同年份植被恢复情况存在一定差异。火灾发生当年（2015年）火烧迹地植被恢复状况高于未发生火灾区域，而在火后第1年（2016年）却又稍低于未发生火灾区域，直到火后第2年（2017年），火烧迹地植被基本恢复到火前状态。同时，不同火烧严重度下的植被恢复过程在存在明显差异。草原火灾发生后当年（2015年），中强度火烧下植被恢复最好，其次是轻度，重度表现最差。草原火烧严重度对植被恢复的影响，主要表现在火后第1个植被生长季。在之后的年份里，由于草原更新能力强大，不同火烧严重度对草原植被的影响不明显。本研究可丰富草原火灾及火烧迹地植被恢复等相关研究成果，同时为草原火灾管理和草原生态系统的可持续发展提供科学依据。     

橡胶园酸性、中性土壤交换性钙、镁测定方法研究

为了获得简单、易操作、准确度高的橡胶园酸性、中性土壤交换性钙、镁测定方法,本试验通过响应面优化试验,建立称样量、提取液体积、提取方式与交换性钙测定结果之间的数学模型。结果表明：在25℃条件下,以1 mol/L的乙酸铵为土壤浸提液,称样量为2 g、提取液体积175 mL、振荡36 min,为橡胶园酸性、中性土壤的最佳提取条件。将上述条件下得到的结果与标准方法条件下得到的结果相对比,两结果的相对误差在1.25~3.04之间,均属合理范围。利用标准样品对优化方法进行试验验证,结果与推荐值相符。改进后的方法分析成本低、操作简便、测定结果准确、稳定性好,可用于橡胶园酸性、中性土壤交换性钙、镁的测定。     

Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.     

Getting cosy in freshwater: Assumed to be brackish pike are not so brackish after all

Pike (Esox lucius) occupy coastal streams and rivers of the Baltic Sea, where they attain large sizes (>5 kg). These large sizes are perhaps due to the fact that they can tolerate relatively high salinities and can thus forage in the nearby more productive brackish environments. In an attempt to quantify the extent to which pike utilise brackish environments, and to provide some insight into the underlying causes for brackish water migrations, we tagged 30 pike from a western Baltic river with acoustic transmitters and were able to track 21 individuals for 1 year. Based on experienced from local anglers, this population was assumed to be brackish in nature, where individuals underwent freshwater migrations to spawn. Our findings however suggest that the smallest and most active individuals make short exits into brackish waters and do so on rare occasions. Our results further indicate that neither sex nor size is related to activity level. We suggest that these patterns reflect two distinct behaviours—active and passive—and that large pike can be supported by the food availability in the river, without the need to venture into coastal zones, thus defying the conventional view that Baltic pike are all brackish in nature.     

豚草粗提物对杨树水泡型溃疡病的抑菌作用研究

为解决豚草提取物在林业病虫害防治中的推广与应用问题,以豚草叶片为试验材料,采用温浸-超声波联合提取法提取抑菌活性物质,并开展其对杨树水泡型溃疡病病原菌的室内外抑菌试验。室内抑菌试验表明,粗提物浓度为16 mg/mL时,抑菌率达到73.62%;林间防治试验表明,最小抑菌浓度为 40 mg/mL,抑菌率达到95.14%。通过以上抑菌试验确定豚草粗提物对杨树水泡型溃疡病有较好的抑制效果。     

超高效液相色谱—串联质谱法检测鸡血液样品中喹诺酮类药物残留

旨在建立鸡血液样品中多种喹诺酮类药物残留的超高效液相色谱—串联质谱方法。样品经乙腈提取,经Waters Acquity UPLC BEH C₁₈色谱柱分离,以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,电喷雾正离子（ESI⁺）模式电离,MRM扫描模式检测。经方法学验证,该方法的线性关系、回收率、精密度均符合要求。应用超高效液相色谱—串联质谱法建立了鸡血液样品中多种喹诺酮类药物同时检测的方法,实现了动物血液样品中12种喹诺酮类药物同时定性、定量分析。     

直播稻田无人机全程防治病虫草害初探

针对不同设备设计不同喷液量,飞行参数验证防治效果,从而验证无人机在稻田全程应用的可能性,旨在探索2种小型多旋翼植保无人机低空喷洒在整个水稻生长时期病虫草害防治过程中的防效。结果表明:当喷洒水田封闭式除草剂的喷洒量为10650、15000 mL/hm²,其对杂草的株防效与鲜重防效与人工电动喷雾相近。在病虫害的防治试验中,使用推荐用量且喷洒量为10650、15000 mL/hm²时,以及使用70%推荐用量（减量30%）且喷洒量为15000 mL/hm²时,防治效果均与人工电动喷雾器无显著差异。通过验证,选择合适的药剂,配合科学的喷洒参数,可在保证药效的前提下减少农药使用量。多旋翼植保无人机在水稻田有进行大面积全程飞防植保应用有广阔的前景。     

Effect of chlorogenic acid on intestinal inflammation,antioxidant status,and microbial community of young hens challenged with acute heat stress

Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA₃₀₀ group and HS + CGA₆₀₀ group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA₃₀₀, and HS + CGA₆₀₀ group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.