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41.
Patricio Arce Mauricio Moreno Mónica Gutierrez Marlene Gebauer Paola Dell’Orto Hebert Torres Ivette Acuña Pauline Oliger Alejandro Venegas Xavier Jordana Julio Kalazich Loreto Holuigue 《American Journal of Potato Research》1999,76(3):169-177
Blackleg and soft rot diseases, caused by the bacteriumErwinia carotovora, are among the diseases that cause important losses in culture and storage of potato. In this paper, we introduced bacterial resistance into potato, via genes encoding for proteins with antibacterial activity. For this purpose, potato clones were transformed either with the gene encoding the acidic attacin protein fromHyalophora cecropia, or with the gene encoding the cecropin analog peptide SB37. These clones were evaluated for soft rot and blackleg resistance, after inoculation with the bacterial strainErwinia carotovora subsp.atroseptica T7. Results reported in this paper indicate that a considerable percentage of the potato clones (15–22%) showed increased resistance to bacterial infection, revealed by reduced severity of blackleg or soft rot symptoms. Expression of the transgenes was demonstrated in some of the clones by Northern blot analysis. This is the first report indicating that expression of the gene encoding for an attacin protein and for the cecropin SB-37 peptide in transgenic potato confers increased resistance to bacterial infection. 相似文献
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Marília Hauser Carolina R.C. Doria Roberto V. Santos Aurea García‐Vasquez Marc Pouilly Christophe Pcheyran Emmanuel Ponzevera Gislene Torrente‐Vilara Sylvain Brail Jacques Panfili Audrey Darnaude Jean‐Franois Renno Carmen García‐Dvila Jesus Nuez Franck Ferraton Gladys Vargas Fabrice Duponchelle 《水产资源保护:海洋与淡水生态系统》2019,29(3):397-408
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Hebert D Eschner A 《Veterinary therapeutics : research in applied veterinary medicine》2010,11(3):E1-E9
The in vitro activity of 10 cephalosporin antimicrobial agents against 75 isolates of methicillin-susceptible Staphylococcus pseudintermedius derived from dogs was assessed. The lowest minimal inhibitory concentration for 90% of strains (MIC90) values obtained were for cephalothin, cefovecin, and cefazolin (0.12 ug/mL), followed by ceftiofur and cefoxitin (0.25 ug/mL), cefpodoxime (0.5 ug/mL), and cefaclor and cefadroxil (1 ug/mL). The highest MIC90 values were found for cephalexin and cefixime (2 ug/mL). In this in vitro study, sensitivity to cephalothin was indicative of cephalexin susceptibility, although there were marked differences in MICs. Cephalothin susceptibility was not indicative of susceptibility to all tested cephalosporins, nor was there a clear trend in susceptibility based on cephalosporin generation. 相似文献
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Bioassays were conducted in Mauritania to determine the toxicity of botanical insecticides from the tree Melia volkensii Gürke (Meliaceae) to ladybird predators (Coleoptera: Coccinellidae) of the date palm scale, Parlatoria planchardi Targ. (Homoptera: Diaspididae). M. volkensii seed extract was formulated in neem oil or a mixture of neem and maize oil. Three preparations were tested on Chilocorus bipustulatus L. var. iranensis, an introduced species, and one on the indigenous Pharoscymnus anchorago F., a species already used in previous bioassays. Fourth instar larvae were exposed for 2 days to treated scale-infested date palm leaves. The botanical insecticides were toxic to C. bipustulatus. Median lethal application rates (LR50s) were close to the recommended application rate of 1 l/ha. In contrast, P. anchorago showed no increased mortality at this rate. Hazard quotients (application rate divided by the LR50) were generally less than 2, suggesting a low risk for both species. However, risk mitigation measures are recommended when using oil formulations because the threshold value for C. bipustulatus, the more susceptible of the two ladybird species, would be exceeded at higher dose rates or when conducting multiple applications. Sublethal effects included an extension of the larval stage and morphogenetic defects. These effects were again more pronounced in C. bipustulatus than in P. anchorago. 相似文献
48.
Efficient plant protection is of fundamental importance in order to warrant food security. Here, we present arguments that a coordinate approach in plant protection is urgently required, taking advantage of a broad mix of measures, including modern synthetic chemistries, capable of protecting plants from adverse organisms.
相似文献49.
Field trials carried out between 1982 and 1984 to evaluate potato clones and cultivars for their resistance to potato smut indicated that from a total of 179 accessions tested seven were resistant during the three-year tests. Five accessions were resistant during two consecutive trials but then there were not enough tubers available for a retest for the third time. Accessions that were resistant during the three consecutive field trials included Peruvian cultivars Cuzco, Mariva, Mi Peru, Participación and Revolución, and CIP’s clones 376181.5 and 6956.52. 相似文献
50.
Diego F. Vasquez Anngie Hernandez Diana Torres Felipe Borrero-Echeverry Paola Zuluaga Diego F. Rincon 《Plant pathology》2022,71(6):1282-1292
Plant–virus interactions are affected by environmental conditions that determine plant vulnerability to pathogens and the population dynamics of insect vectors. We hypothesize that drought enhances horizontal transmission by dampening the basal immunity of plants, which triggers symptom expression and vector manipulation. The potato yellow vein virus (PYVV) causes potato yellow vein disease (PYVD), a re-emerging epidemic of potato crops in South America, and is transmitted horizontally by the greenhouse whitefly (GWF), Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), or vertically through infected seed tubers. We investigated the role of drought and temperature as modulators of PYVD symptom expression, plant immune response, and vector survival, development and host preference. We found that drought induced symptom expression, suppressed the salicylic acid pathway and increased PYVV replication. GWF survival was reduced on PYVV-infected hosts and development was slowest when they fed on plants with PYVD symptoms, which also triggered adults’ attraction to PYVV-infected plants. However, adults previously fed on infected plants showed the opposite effect, being more attracted to PYVV-free plants. We propose a theoretical model that explains the role of drought in modulating potato–PYVV–GWF interactions and provides new insights into plant–virus–vector coevolution. 相似文献