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Optimised methodology for the extraction of protein from quinoa (Chenopodium quinoa Willd.)
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Manuel R. Guerreo‐Ochoa Romina Pedreschi Rosana Chirinos 《International Journal of Food Science & Technology》2015,50(8):1815-1822
Quinoa is a highly appreciated Andean pseudo‐cereal and has sparked attention worldwide due to its excellent nutritional value. The protein extraction parameters for defatted quinoa seed meal (DQSM) were optimised in this study. Initially, a Plackett–Burman design was applied to screen the factors displaying a potential effect on the quinoa protein extraction yield (Y %, g soluble protein/100 g total protein) being the main evaluated factors: pH, NaCl concentration, time, temperature, solvent type, particle size and solvent/meal ratio. Four main factors: temperature, solvent/meal ratio, pH and time selected from the screening step were optimised with a central composed design (CCD). The obtained response surface model (RSM) produced a satisfactory fitting of the results (R2 = 0.9308). Optimal quinoa protein extraction conditions of 36.2 °C, solvent/meal ratio of 19.6/1 (v/w) and 90 min resulted in a protein yield of 62.1% (9.06 g of protein/100 g DQSM) which closely agree with the predicted value of 62.5%.The model was experimentally validated by extracting the quinoa protein using the optimal conditions revealed by RSM. The optimised conditions could be successfully employed in the design process of protein extraction from quinoa seed meal to obtain optimal yields. 相似文献
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Giacomo Lazzarino Romina Pallisco Gabriele Bilotta Ilaria Listorti Renata Mangione Miriam Wissam Saab Giuseppe Caruso Angela Maria Amorini Maria Violetta Brundo Giuseppe Lazzarino Barbara Tavazzi Pasquale Bilotta 《International journal of molecular sciences》2021,22(16)
Nearly 40–50% of infertility problems are estimated to be of female origin. Previous studies dedicated to the analysis of metabolites in follicular fluid (FF) produced contrasting results, although some valuable indexes capable to discriminate control groups (CTRL) from infertile females (IF) and correlate with outcome measures of assisted reproduction techniques were in some instances found. In this study, we analyzed in blind FF of 35 control subjects (CTRL = patients in which inability to obtain pregnancy was exclusively due to a male factor) and 145 IF (affected by: endometriosis, n = 19; polycystic ovary syndrome, n = 14; age-related reduced ovarian reserve, n = 58; reduced ovarian reserve, n = 29; unexplained infertility, n = 14; genetic infertility, n = 11) to determine concentrations of 55 water- and fat-soluble low molecular weight compounds (antioxidants, oxidative/nitrosative stress-related compounds, purines, pyrimidines, energy-related metabolites, and amino acids). Results evidenced that 27/55 of them had significantly different values in IF with respect to those measured in CTRL. The metabolic pattern of these potential biomarkers of infertility was cumulated (in both CTRL and IF) into a Biomarker Score index (incorporating the metabolic anomalies of FF), that fully discriminated CTRL (mean Biomarker Score value = 4.00 ± 2.30) from IF (mean Biomarker Score value = 14.88 ± 3.09, p < 0.001). The Biomarker Score values were significantly higher than those of CTRL in each of the six subgroups of IF. Posterior probability curves and ROC curve indicated that values of the Biomarker Score clustered CTRL and IF into two distinct groups, based on the individual FF metabolic profile. Furthermore, Biomarker Score values correlated with outcome measures of ovarian stimulation, in vitro fertilization, number and quality of blastocysts, clinical pregnancy, and healthy offspring. These results strongly suggest that the biochemical quality of FF deeply influences not only the effectiveness of IVF procedures but also the following embryonic development up to healthy newborns. The targeted metabolomic analysis of FF (using empowered Redox Energy Test) and the subsequent calculation of the Biomarker Score evidenced a set of 27 low molecular weight infertility biomarkers potentially useful in the laboratory managing of female infertility and to predict the success of assisted reproduction techniques. 相似文献
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Cuculayev R Sanyal A Atay NZ Culha M Bucak S 《Journal of nanoscience and nanotechnology》2012,12(3):2761-2766
The self-assembly of magnetic nanoparticles into higher-order organizations upon external magnetic stimulation has critical importance for the fabrication of discrete microstructures. In this study, the tuning of self-assembly behavior of magnetic Fe3O4 nanoparticles (MNPs), with an average size of 6 nm, under the enhanced magnetic force upon changing the applied field strength and direction is explored. Upon evaporation of the solvent where the MNPs are suspended, formation of particular micrometer sized structures is achieved with a surface constructed from sub-micrometer size magnetic beads in between the applied magnetic field and MNPs. In this study, three different surfaces fabricated using sub-micrometer size magnetic beads in between the applied magnetic field and MNPs are used and the effect of the template pattern, applied field strength and direction are explored. 相似文献
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Stability of fructooligosaccharides,sugars and colour of yacon (Smallanthus sonchifolius) roots during blanching and drying
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David Campos Ana Aguilar‐Galvez Romina Pedreschi 《International Journal of Food Science & Technology》2016,51(5):1177-1185
Yacon (Smallanthus sonchifolius) root is an important source of fructooligosaccharides (FOS). This study evaluated the influence of the blanching and drying processes on the sugars, FOS and colour of the obtained flour. Blanching in boiling water of 5 mm slices for 6 min allowed to inactivate 95% of polyphenol oxidase and peroxidase activity. Blanching solutions containing ascorbic, citric and lactic acid were detrimental in terms of FOS retention (68.2–87.4%) due to hydrolysis mainly of GF3, GF4 and GF5 FOS, and also important losses of reducing sugars (RS) were observed (69.5–87.4% retention). Blanching treatments that included ascorbic acid/CaCl2 prevented RS and FOS losses and improved colour of the obtained flour. The drying tested temperatures of 50–80 °C did not affect the RS retention and FOS losses associated to hydrolysis and the use of 80 °C rapidly reduced the water content and minimised browning reactions yielding flours with excellent colour characteristics with high FOS content that can be derived to the elaboration of prebiotic containing functional foods or for the extraction and purification of FOS. 相似文献
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Romina Bellingeri Fabrisio Alustiza Natalia Picco Diego Acevedo Maria Alejandra Molina Rebeca Rivero Carolina Grosso Carlos Motta Cesar Barbero Adriana Vivas 《应用聚合物科学杂志》2015,132(5)
Composite materials based on carbon nanotubes (CNT) and polymeric hydrogels have become the subject matter of major interest for use as carriers in drug delivery research. The aim of this study was to evaluate the in vitro cytotoxicity of the hydrogel–carbon nanotube–chitosan (hydrogel–CNT–CH) composites on intestinal cells. Oxidized CNT were wrapped with chitosan (CH), Fourier transform infrared (FT‐IR) analysis suggest that oxidized CNT interact with CH. Transmission electron microscopy (TEM) images show a CH layer lying around CNT. Chitosan wrapped CNT were incorporated to poly (acrylamide‐co‐acrylic acid) hydrogels. Swelling behavior in buffers at different pH were evaluated and revealed a significantly lower swelling when it is exposed to a acid buffer solution (pH 2.2). Mechanical properties were evaluated by measurements of elasticity and the material with CNT showed better mechanical properties. The incorporation and liberation of Egg Yolk Immunoglobulin from hydrogel–CNT–CH were also assessed and it revealed an improved performance. To evaluate the effect of these nanocomposites on cellular redox balance, intestinal cells were exposed to hydrogel–CNT–CH composites and antioxidant enzymes were assessed. Cytotoxicity and apoptosis were also evaluated. Hydrogel–CNT–CH composites induce no oxidative stress and there were no evidence of cytotoxicity or cell death. These preliminary findings suggest that hydrogel–CNT–CH composites show improved properties and good biocompatibility in vitro making these biomaterials promising systems for drug delivery purposes. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41370. 相似文献
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Romina Zappacosta Monica Semeraro Massimo Baroncini Serena Silvi Massimiliano Aschi Alberto Credi Antonella Fontana 《Small (Weinheim an der Bergstrasse, Germany)》2010,6(8):952-959
The effect of the luminescent heteroaromatic electron acceptor N,N′‐dimethyl‐2,7‐diazapyrenium dichloride (DM‐DAP2+) on the stability of 1‐palmitoyl‐2‐oleoylphosphatydilcholine (POPC) liposomes is determined on the basis of the rate of release of different fluorescent probes entrapped within the liposome. The experiments show that DM‐DAP2+ exerts a substantial destabilizing action on the liposomal bilayer, particularly at low concentrations. Molecular dynamics simulations suggest that the activity of DM‐DAP2+ is related to its tendency to surround itself with water molecules, conceivably favoring the formation of transient pores across the bilayer. 相似文献
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Rosana Chirinos Jorge GalarzaIndira Betalleluz-Pallardel Romina Pedreschi David Campos 《Food chemistry》2010
The antioxidant capacities of ascorbic acid and phenolic compounds present in camu camu fruit were screened during ripening. Ascorbic acid decreased, and anthocyanin, flavonol and flavanol contents, and DPPH antioxidant capacity increased during ripening. Antioxidant compounds from camu camu were fractionated in two fractions: an ascorbic acid-rich fraction (F-I) and a phenolics-rich fraction (F-II). F-I was the major contributor to the DPPH antioxidant capacity (67.5–79.3%) and F-II played a minor role (20.7–32.5%). A total of 30 different phenolic compounds were detected by HPLC-PAD. The presence of catechin, delphinidin 3-glucoside, cyanidin 3-glucoside, ellagic acid and rutin was elucidated. Other phenolic compounds, such as flavan-3-ol, flavonol, flavanone and ellagic acid derivatives, were also present. For the three ripening stages the flavan-3-ols and ellagic acid group were the most representative phenolic compounds in this fruit. Acid hydrolysis of F-II revealed the presence mainly of gallic and ellagic acids, suggesting that camu camu fruit possesses important quantities of hydrolysed tannins (gallo- and/or ellagitannins). These results confirm that camu camu fruit is a promising source of antioxidant phenolics. 相似文献