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61.
62.
Federico Bolognesi Nicola Fazio Filippo Boriani Viscardo Paolo Fabbri Davide Gravina Francesca Alice Pedrini Nicoletta Zini Michelina Greco Michela Paolucci Maria Carla Re Sofia Asioli Maria Pia Foschini Antonietta DErrico Nicola Baldini Claudio Marchetti 《International journal of molecular sciences》2022,23(3)
Defects of the peripheral nervous system are extremely frequent in trauma and surgeries and have high socioeconomic costs. If the direct suture of a lesion is not possible, i.e., nerve gap > 2 cm, it is necessary to use grafts. While the gold standard is the autograft, it has disadvantages related to its harvesting, with an inevitable functional deficit and further morbidity. An alternative to autografting is represented by the acellular nerve allograft (ANA), which avoids disadvantages of autograft harvesting and fresh allograft rejection. In this research, the authors intend to transfer to human nerves a novel technique, previously implemented in animal models, to decellularize nerves. The new method is based on soaking the nerve tissues in decellularizing solutions while associating ultrasounds and freeze–thaw cycles. It is performed without interrupting the sterility chain, so that the new graft may not require post-production γ-ray irradiation, which is suspected to affect the structural and functional quality of tissues. The new method is rapid, safe, and inexpensive if compared with available commercial ANAs. Histology and immunohistochemistry have been adopted to evaluate the new decellularized nerves. The study shows that the new method can be applied to human nerve samples, obtaining similar, and, sometimes better, results compared with the chosen control method, the Hudson technique. 相似文献
63.
Mechanical behavior of cold-water fish gelatin gels crosslinked with 1,4-butanediol diglycidyl ether
Marina Czerner Mariano Prudente Josefa Fabiana Martucci Federico Rueda Laura Alejandra Fasce 《应用聚合物科学杂志》2020,137(34):48985
In this work, chemical crosslinking with 1,4-butanediol diglycidyl ether (BDDGE) is used as strategy to enhance mechanical performance of fish gelatin (FG) gels in order to meet the properties' range of mammalian gelatin physical gels. Joint analysis of free amino groups, swelling ratio, and total soluble material indicates that crosslinking degree increases with increasing FG concentration and it is favored by a 0.2 BDDGE/FG ratio. Increasing crosslinking degree enhances gel indentation strength and shear modulus (μ) while decreases fracture toughness (GIC). Measured μ and GIC values lies within the range exhibited by mammalian gelatin physical gels, but the relationship between these parameters is opposite. This is due to the different fracture mechanisms occurring in chemically crosslinked and physical gels. 相似文献
64.
65.
The effect of dietary lipid sources (animal fat, AF vs. vegetable oil, VO) was investigated on the phospholipid (PL) fraction
of raw chicken meat and pre-cooked chicken patties. PL classes were determined on extracted lipids by high-performance liquid
chromatography (HPLC) connected with an evaporative light-scattering detector (ELSD). The HPLC-ELSD method showed good intraday
repeatability (less than 5% for different PL) and limits of detection ranging from 0.006 mg/mL (phosphatidylethanolamine)
to 0.045 mg/mL (sphingomyelin). Diet did not consistently influence PL in chicken breast. A significant difference was assessed
in meat batter where total PL were at levels of 349.0 and 388.2 mg/100 g of product in batter from broilers fed AF and VO
diet, respectively. Cooking oil absorption caused an important decrease in PL (lower than 300 mg/100 g of product in pre-fried
patties). Diet was an effective means to control the PL fatty acid profile in chicken, particularly the levels of polyunsaturated
fatty acids (PUFA). In minced breast and thigh and meat batter from birds fed a VO-supplemented diet, the relative amount
of PUFA was 46, 81 and 28% higher than that determined in the corresponding samples obtained from broilers fed an AF-based
diet. 相似文献
66.
67.
Corrado Costa Paolo Menesatti Jacopo Aguzzi Stefano D’Andrea Francesca Antonucci Valentina Rimatori Federico Pallottino Marco Mattoccia 《Food and Bioprocess Technology》2010,3(1):43-48
The actual Italian production of clams is chiefly sustained by the native Tapes decussatus and the fortuitously imported Tapes philippinarum. Both species are commercialized as “Vongola verace”, but the commercial value of T. philippinarum is lower. The discrimination of species by sight is usually difficult and it cannot be done by observation based on shell
morphology but only when animals open their valves hence displaying the two siphons. In this study, we propose a new, noninvasive
method to discriminate individuals of both species based on the analysis of the external shape of their shells. Accordingly,
in sympatric populations at two sites of the Po river outlet, we have chosen individuals (63 for T. decussatus and 57 for T. philippinarum) of comparable commercial size for which a certain genetic discrimination was previously done. Pictures of the left side
valve were taken for all specimens. Their profiles were analyzed with the elliptic Fourier analysis. The mean outline for
each species was graphically extracted. The coefficients of the harmonic equations were analyzed by multivariate classification
(partial least squares discriminant analysis [PLSDA]). Results showed a high percentage of correct classification of individuals
of both species (96.6%). Contour analysis reflected the overall shell shape and thus identified morphological aspects that
were difficult to recognize and quantify in sight. The high percentage of correct classifications obtained by combining the
analysis of elliptic Fourier harmonics with PLSDA demonstrated the feasibility of this method to discriminate species with
a high level of resemblance. 相似文献
68.
A New Design Paradigm for Smart Windows: Photocurable Polymers for Quasi‐Solid Photoelectrochromic Devices with Excellent Long‐Term Stability under Real Outdoor Operating Conditions 下载免费PDF全文
Federico Bella George Leftheriotis Gianmarco Griffini George Syrrokostas Stefano Turri Michael Grätzel Claudio Gerbaldi 《Advanced functional materials》2016,26(7):1127-1137
A new photoelectrochromic device (PECD) is presented in this work proposing the combination of a WO3‐based electrochromic device (ECD) and a polymer‐based dye‐sensitized solar cell (DSSC). In the newly designed architecture, a photocurable polymeric membrane is employed as quasi‐solid electrolyte for both the ECD and the DSSC. In addition, a photocurable fluoropolymeric system is incorporated as solution‐processable external protective thin coating film with easy‐cleaning and UV‐shielding functionalities. Such new polymer‐based device assembly is characterized by excellent device operation with improved photocoloration efficiency and switching ability compared with analogous PECDs based on standard liquid electrolyte systems. In addition, long‐term (>2100 h) stability tests under continuous exposure to real outdoor conditions reveal the remarkable performance stability of this new quasi‐solid PECD system, attributed to the protective action of the photocurable fluorinated coating that effectively prevents photochemical and physical degradation of the PECD components during operation. This first example of quasi‐solid PECD systems paves the way for a new generation of thermally, electrochemically, and photochemically stable polymer‐based PECDs, and provides for the first time a clear demonstration of their true potential as readily upscalable smart window components for energy‐saving buildings. 相似文献
69.
Tonino Liserra Marco Maglionico Valentina Ciriello Vittorio Di Federico 《Water Resources Management》2014,28(5):1201-1217
Reliability of water distribution networks (WDNs) has received much attention in recent years due to progressive aging of infrastructures and climate change. Several reliability indicators, focusing on hydraulic aspects rather than water quality, have been proposed in literature. Reliability is generally assessed resorting to well established methods coupling hydraulic simulations and stochastic techniques that describe the WDNs hydraulic performance and component availability respectively. Two main algorithms are employed to simulate WDNs: the demand driven approach (DDA) that disregards the physical relationship between actual water demand and nodal pressure, and the pressure driven approach (PDA) that explicitly incorporates it. In this paper, we show how the choice of hydraulic solver may affect reliability indicators. We modify existing quantitative indicators at nodal and network level, and define novel indicators to consider water quality aspects. These indicators are evaluated for three example WDNs; discrepancies between results obtained with the two approaches depend on network size, feeding scheme and skeletonization. Results suggest to use with caution the DDA for reliability assessment at both local and global level. 相似文献
70.
Federico C. Buroni Jhonny E. Ortiz Andrés Sáez 《International journal for numerical methods in engineering》2011,86(9):1125-1143
In this paper we develop an alternative boundary element method (BEM) formulation for the analysis of anisotropic three‐dimensional (3D) elastic solids. Our implementation is based on the derivation of explicit expressions for the fundamental solution displacements and tractions, of general validity for any class of anisotropic materials, by means of Stroh formalism and Cauchy's residue theory. The resulting fundamental solution remains valid for mathematical degenerate cases when Stroh's eigenvalues are coincident, meanwhile it does not exhibit numerical instabilities for quasi‐degenerate cases when Stroh's eigenvalues are nearly equal. A multiple pole residue approach is followed, leading to general explicit expressions to evaluate the traction fundamental solution for poles of m‐multiplicity. Despite the existence of general displacement solutions in the literature, and for the sake of completeness, the same approach as for the traction solution is considered to derive the displacement fundamental solution as well. Based on these solutions, an explicit BEM approach for the numerical solution of 3D linear elastic problems for solids with general anisotropic behavior is presented. The analysis of cracked anisotropic solids is also considered. Details on the numerical implementation and its validation for degenerate cases are discussed. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献