In vitro synergism of antimutagenic and antioxidant activities of Phoenix dactylifera fruit

The availability of total phenolics of date flesh (Pheonix dactylifera) in aqueous and acidified methanol and in vitro synergism of their antimutagenic and antioxidant activities were investigated. The antimutagenic activities of date flesh extracts tested against Salmonella strains TA-98 and TA-100 using Ames bacterial testing were in the range of 36.47 to 79.74% against the standard mutagens potassium dichromate and sodium azide. The antioxidant activities assessed as maximal inhibitory concentrations (IC₅₀) for DPPH radicals and linoleic acid peroxidation inhibition were 812.90 to 2,076.10 μg/mL and 54.43 to 80.89%, respectively. The total phenolic contents of date flesh extracts (35.76 to 114.09mg/g of gallic acid equivalents) were found to be correlated with the biological activities. Use of 0.5 N acidified methanol was efficient for extraction of phenolic compounds with retained antioxidant and antimutagen activities. Dates are a good candidate as a source for development of chemotherapeutic drugs, nutracueticals, and functional food ingredients.     

Palyno‐morphological characteristics of gymnosperm flora of pakistan and its taxonomic implications with LM and SEM methods

The present study is intended to assess gymnosperms pollen flora of Pakistan using Light Microscope (LM) and Scanning Electron Microscopy (SEM) for its taxonomic significance in identification of gymnosperms. Pollens of 35 gymnosperm species (12 genera and five families) were collected from its various distributional sites of gymnosperms in Pakistan. LM and SEM were used to investigate different palyno‐morphological characteristics. Five pollen types (i.e., Inaperturate, Monolete, Monoporate, Vesiculate‐bisaccate and Polyplicate) were observed. Six In equatorial view seven types of pollens were observed, in which ten species were sub‐angular, nine species were Traingular, six species were Perprolate, three species were Rhomboidal, three species were semi‐angular, two species were rectangular and two species were prolate. While five types of pollen were observed in polar view, in which ten species were Spheroidal, nine species were Angular, eight were Interlobate, six species were Circular, two species were Elliptic. Eighteen species has rugulate and 17 species has faveolate ornamentation. Eighteen species has verrucate and 17 have gemmate type sculpturing. The data was analysed through cluster analysis. The study showed that these palyno‐morphological features have significance value in classification and identification of gymnosperms. Based on these different palyno‐morphological features, a taxonomic key was proposed for the accurate and fast identifications of gymnosperms from Pakistan.     

Wild melon: a novel non-edible feedstock for bioenergy

In the present research work, a non-edible oil source Cucumis melo var. agrestis(wild melon) was systematically identified and studied for biodiesel production and its characterization. The extracted oil was 29.1% of total dry seed weight. The free fatty acid value of the oil was found to be 0.64%, and the single-step alkaline transesterification method was used for conversion of fatty acids into their respective methyl esters. The maximum conversion efficiency of fatty acids was obtained at 0.4 wt% Na OH(used as catalyst), 30%(methanol to oil, v/v) methanol amount, 60 ℃ reaction temperature,600-rpm agitation rate and 60-min reaction time. Under these optimal conditions, the conversion efficiency of fatty acid was 92%. However, in the case of KOH as catalyst, the highest conversion(85%) of fatty acids was obtained at 40%methanol to oil ratio, 1.28 wt% KOH, 60 ℃ reaction temperature, 600-rpm agitation rate and 45 min of reaction time.Qualitatively, biodiesel was characterized through Fourier transform infrared spectroscopy(FTIR) and gas chromatography and mass spectroscopy(GC–MS). FTIR results demonstrated a strong peak at 1742 cm~(-1), showing carbonyl groups(C=O)of methyl esters. However, GC–MS results showed the presence of twelve methyl esters comprised of lauric acid, myristic acid, palmitic acid, non-decanoic acid, hexadecanoic acid, octadecadienoic acid and octadecynoic acid. The fuel properties were found to fall within the range recommended by the international biodiesel standard, i.e., American Society of Testing Materials(ASTM): flash point of 91 ℃, density of 0.873 kg/L, viscosity of 5.35 c St, pour point of-13 ℃, cloud point of-10 ℃, total acid number of 0.242 mg KOH/g and sulfur content of 0.0043 wt%. The present work concluded the potential of wild melon seed oil as excellent non-edible source of bioenergy.     

Phonon phase stability,structural, mechanical,electronic, and thermoelectric properties of two new semiconducting quaternary Heusler alloys CoCuZrZ (Z = Ge and Sn)

For meeting the energy demand, the development of new and novel thermoelectric (TE) materials for power generation is very vital. In this draft, we have theoretically investigated two new quaternary CoCuZrZ (Z = Ge and Sn) Heusler alloys for their structural, mechanical, electronic, and TE properties. In the energy minimization process, the alloys are found to be non-magnetic in the ground state. Based on calculated phonon dispersion curves, formation energy, and elastic constants, we propose that both CoCuZrGe and CoCuZrSn are stable. Furthermore, the mechanical properties indicate that CoCuZrGe (CoCuZrSn) has a brittle (ductile) nature. The electronic properties examined in Perdew-Burke-Ernzerhof (PBE), PBEsol, and modified Becke-Johnson (mBJ) potential, all predict that reported systems are narrow-gap semiconductors (SCs). In addition, the temperature dependent TE properties have been studied by calculating the electronic thermal conductivity (κ), Seebeck coefficient (S), power factor (PF) and electrical conductivity (σ/τ). The obtained positive value of S conveys the materials as p-type SCs, with a maximum value of 26.2 μV/K for CoCuZrGe and 28 μV/K for CoCuZrSn. The σ/τ, κ, and PF show increasing trends with rising temperature. The PF is found to be 1.55 × 10¹² WK⁻²m⁻¹s⁻¹ for CoCuZrGe and 1.38 × 10¹² WK⁻²m⁻¹s⁻¹ for CoCuZrSn. The proposed semiconducting Heusler alloys may receive attention for a range of TE and spintronic applications.     

Recent Applications of Polymer Blends in Gas Separation Membranes

Polymeric membranes are extensively used for gas separations but their performance is limited by the upper bound trade‐off discovered by Robeson in 1991. Among the attractive modifications available to increase the performance of polymeric membranes, polymer blending is a unique technique because it offers a time‐ and cost‐effective method of tuning the properties of membranes. A variety of polymer blends has been explored in recent years. The application of polymer blends in gas separation membranes is described by critically analyzing the performance of polymer blend membranes. Polymer blend membranes of different polymer pairs are reviewed and evaluated in terms of phase behavior, permeability, and selectivity.     

Lysine fortification of wheat flour improves selected indices of the nutritional status of predominantly cereal-eating families in Pakistan

Wheat provides more than 50% of the protein and calorie intake of the population of Pakistan. Legumes and animal protein that could complement the amino acid pattern of wheat, in which lysine is the first limiting amino acid for utilization of protein, are not affordable by members of lower socioeconomic groups in developing countries. The purpose of the study was to determine whether lysine fortification of wheat flour would have a positive impact on populations consuming a predominantly wheat-based diet. A double-blind study was carried out for three months on the outskirts of Peshawar, Pakistan. Forty families received wheat flour fortified with lysine, and 40 families received wheat flour without lysine. Wheat provided 59% of the protein for men, 65% for women, and 58% for children. The weight and height of the children in both groups increased during the study, but the increase was significantly greater in the lysine group. Hemoglobin increased significantly in the women receiving lysine-fortified flour. Transferrin levels increased significantly in men, women, and children in the lysine group as compared with those in the control group. Prealbumin increased significantly in adults receiving additional lysine but decreased in children. Men, women, and children in the lysine-supplemented families had significant increases in CD4, CD8, and complement C3 as compared with controls. These results indicate that lysine fortification of wheat flour can significantly improve sensitive indicators of nutritional status in a population consuming a diet in which 58% to 65% of the protein, depending on age and sex, is supplied by wheat.     

Pollen morphological investigations of family Cactaceae and its taxonomic implication by light microscopy and scanning electron microscopy

The family Cactaceae is the diversified group of angiosperm plants whose pollen statistics has been used for taxonomic identification. In this article, we present the pollen morphology of eight species belong to seven taxonomically complex genera of Cactaceae including Astrophytum, Cylindropuntia, Echinocereus, Echinopsis, Mammillaria, Opuntia, and Thelocactus using light and scanning electron microscopy. The pollen grains were acetolyzed, measured, described, and electron photomicrographs were taken. Cactaceae can be characterized by presenting different palynomorphological features including pollen type, sculpturing, polar and equatorial diameter, aperture orientation, exine thickness, P/E ratio, and echini features. Four types of pollen shapes, that is, prolate spheroidal (three species), subprolate (two species), prolate (two species), and oblate spheroidal in Echinocereus reichenbachii were observed. The polar and equatorial diameter observed maximum in O. ficus indica 116.95 and 112.27 μm while minimum in M. compressa 38.42 and 21.05 μm. Pollen of two types, tricolpate in members of subfamily Cactioideae and pantoporate in the Opuntioideae were examined. The fertility percentage has been observed maximum in Opuntia macrocentra (83.84%) and minimum in Opuntia ficus‐indica (57.89%). Exine sculpturing showing great variations such as granulate, reticulate, granulate perforate and micro‐echinate foveolate ornamentation was examined only in Echinopsis eyriesii. A key to species, based on pollen micromorphological attributes, has been constructed for correct identification of complex cactus species.