The effect of low‐power microwaves on the growth of bacterial populations in a plug flow reactor

A plug flow reactor (PFR) which permits the growth of bacteria in a microwave environment is described. The apparatus is used to analyse the effect of microwave radiation at the frequency of 2.45 GHz and at 37°C on the growth rate of three bacterial species: Bacillus clausii, Pseudomonas aeruginosa, and Staphylococcus aureus. The growth constant is determined for reacting mixtures irradiated with microwaves of different powers ranging from 0 to 400 mW. Analyses show that (a) the apparatus is able to perform the experimental runs maintaining an aseptic environment; (b) under the microwave power levels examined in the present study, no effects are detected for the Bacillus clausii and Pseudomonas aeruginosa species, while for the Staphylococcus aureus species, a few small effects are found (a minimum value of the growth constant at 200 mW and an increase of the constant between 200 and 400 mW). © 2009 American Institute of Chemical Engineers AIChE J, 2010     

Recovery of anthocyanins from eggplant peel

Tartaric and malic acid solutions were tested to extract anthocyanins from eggplant peel by a discontinuous process to obtain a natural red colorant. Extraction optimization was carried out, using different solvents, acid concentration, temperature, time of extraction and solvent-to-solid ratio as independent variables. Tartaric acid was more efficient than malic acid in both extraction yield and rate. Comparative tests were carried out using acidified ethanol as solvent. Delphinidin-3-rutinoside was extracted and identified as the major anthocyanin in eggplant peel. Concentration of different extracts from eggplant peel was carried out using EXA-31, a methacrylic food grade resin, the best performing resin to obtain highly concentrated extracts.     

Vinylene-linked pyridine-pyrrole donor–acceptor conjugated polymers

Vinylene-linked donor–acceptor–donor semiconducting polymers have been prepared by electropolymerization of the new monomers 2,6-bis[(E)-2-(1-methylpyrrol-2-yl)vinyl]pyridine and 2,5-bis[(E)-2-(1-methylpyrrol-2-yl)vinyl]pyridine. The monomers, consisting of two pyrrole donor units and a central pyridine acceptor ring spaced by vinylene links, differ by the substitution pattern around the central pyridine core (2,6 vs. 2,5-substitution). The electropolymerization process is more efficient on the 2,5-derivative. The new polymers show reversible electrochemistry dominated by a strong charge trapping effect. Poly(2,5-bis[(E)-2-(1-methylpyrrol-2-yl)vinyl]pyridine) energy levels closely match those of design rules for optimized polymers in combination with fullerene derivatives in bulk heterojunction solar cells.     

Linux real-time framework for fusion devices

A new framework for the development and execution of real-time codes is currently being developed and commissioned at JET. The foundations of the system are Linux, the Real Time Application Interface (RTAI) and a wise exploitation of the new i386 multi-core processors technology.The driving motivation was the need to find a real-time operating system for the i386 platform able to satisfy JET Vertical Stabilisation Enhancement project requirements: 50 μs cycle time. Even if the initial choice was the VxWorks operating system, it was decided to explore an open source alternative, mostly because of the costs involved in the commercial product.The work started with the definition of a precise set of requirements and milestones to achieve: Linux distribution and kernel versions to be used for the real-time operating system; complete characterization of the Linux/RTAI real-time capabilities; exploitation of the multi-core technology; implementation of all the required and missing features; commissioning of the system.Latency and jitter measurements were compared for Linux and RTAI in both user and kernel-space. The best results were attained using the RTAI kernel solution where the time to reschedule a real-time task after an external interrupt is of 2.35 ± 0.35 μs. In order to run the real-time codes in the kernel-space, a solution to provide user-space functionalities to the kernel modules had to be designed. This novel work provided the most common functions from the standard C library and transparent interaction with files and sockets to the kernel real-time modules. Kernel C++ support was also tested, further developed and integrated in the framework.The work has produced very convincing results so far: complete isolation of the processors assigned to real-time from the Linux non real-time activities, high level of stability over several days of benchmarking operations and values well below 3 μs for task rescheduling after external interrupt. From being the alternative option, RTAI has been finally chosen as the platform for the project. A first stable version of the framework has been integrated on the JET system and is already being commissioned. It will be soon be used on the Vertical Stabilisation Enhancement for the Plasma Control Upgrade (PCU) project at JET.     

Fingerprint Clustering with Bounded Number of Missing Values

The problem of clustering fingerprint vectors with missing values is an interesting problem in Computational Biology that has been proposed in Figueroa et al. (J. Comput. Biol. 11(5):887–901, 2004). In this paper we show some improvements in closing the gaps between the known lower bounds and upper bounds on the approximability of variants of the biological problem. Moreover, we have studied two additional variants of the original problem proposed in Figueroa et al. (Proc. 11th Computing: The Australasian Theory Symposium (CATS), CRPIT, vol. 41, pp. 57–60, 2005). We prove that all such problems are APX-hard even when each fingerprint contains only two unknown positions and we present a greedy algorithm that has constant approximation factors for these variants. Despite the hardness of these restricted versions of the problem, we show that the general clustering problem on an unbounded number of missing values such that they occur for every fixed position of an input vector in at most one fingerprint is polynomial time solvable.     

Charge carrier transport anisotropy in ultrananocrystalline diamond films

The optoelectronic properties of ultrananocrystalline diamond films (UNCD) grown using N₂ = 0 and 5% in the deposition gas mixture, are investigated by transient photocurrent measurements under nanosecond light pulses, both in planar and sandwich contact arrangements.Independent of contact configuration and N₂% value, very similar characteristic times in the 6-7 ns range are detected in the nanosecond range, reflecting a homogeneous distribution of states responsible for such decay times. On a longer time scale, nitrogen addition appears to slow down carrier transport promoting trapping and detrapping processes responsible for single and two power law photocurrent decays in films deposited using N₂ = 5% for sandwich and planar contact arrangements, respectively. Such a result suggests a nitrogen induced transport anisotropy tentatively related to structural modifications occurring at relatively low N₂%.     

Spatial resolution enhancement of ultrasound images using neural networks

Spatial resolution in modern ultrasound imaging systems is limited by the high cost of large aperture transducer arrays, which require a large number of transducer elements and electronic channels. A new technique to enhance the spatial resolution of pulse-echo imaging systems is presented. The method attempts to build an image that could be obtained with a transducer array aperture larger than that physically available. We consider two images of the same object obtained with two different apertures, the full aperture and a subaperture, of the same transducer. A suitable artificial neural network (ANN) is trained to reproduce the relationship between the image obtained with the transducer full aperture and the image obtained with a subaperture. The inputs of the neural network are portions of the image obtained with the subaperture (low resolution image), and the target outputs are the corresponding portions of the image produced by the full aperture (high resolution image). After the network is trained, it can produce images with almost the same resolution of the full aperture transducer, but using a reduced number of real transducer elements. All computations are carried out on envelope-detected decimated images; for this reason, the computational cost is low and the method is suitable for real-time applications. The proposed method was applied to experimental data obtained with the ultrasound synthetic aperture focusing technique (SAFT), giving quite promising results. Realtime implementation on a modern, full-digital echographic system is currently being developed.     

Using 3D Sound to Improve the Effectiveness of the Advanced Driver Assistance Systems

Future generation cars will be characterized by a wide range of Information Technology (IT) services providing safety and infotainment. This makes the car an information intensive environment where the visual channel is overloaded, putting the safety of drivers and passengers in jeopardy. We propose the use of a 3D auditory display to provide information from the Advanced Driver Assistance Systems. This reduces the eye-off-road time, exploiting the human capability to associate sounds with positions in space. Preliminary lab tests reveal the suitability of this approach. The system still has to be carefully tuned and personalized to achieve usability and reliability, but we think that it provides a complementary channel that is specially useful in low visibility conditions.     

A non-destructive technique for the determination of mortar load capacityin situ

Materials and Structures - The determination of mortar load capacity in masonryin situ is not an established technique. An indirect method is described in this paper and, like the earlier one by...     

Dynamic analyses of information encoding in neural ensembles

Neural spike train decoding algorithms and techniques to compute Shannon mutual information are important methods for analyzing how neural systems represent biological signals. Decoding algorithms are also one of several strategies being used to design controls for brain-machine interfaces. Developing optimal strategies to design decoding algorithms and compute mutual information are therefore important problems in computational neuroscience. We present a general recursive filter decoding algorithm based on a point process model of individual neuron spiking activity and a linear stochastic state-space model of the biological signal. We derive from the algorithm new instantaneous estimates of the entropy, entropy rate, and the mutual information between the signal and the ensemble spiking activity. We assess the accuracy of the algorithm by computing, along with the decoding error, the true coverage probability of the approximate 0.95 confidence regions for the individual signal estimates. We illustrate the new algorithm by reanalyzing the position and ensemble neural spiking activity of CA1 hippocampal neurons from two rats foraging in an open circular environment. We compare the performance of this algorithm with a linear filter constructed by the widely used reverse correlation method. The median decoding error for Animal 1 (2) during 10 minutes of open foraging was 5.9 (5.5) cm, the median entropy was 6.9 (7.0) bits, the median information was 9.4 (9.4) bits, and the true coverage probability for 0.95 confidence regions was 0.67 (0.75) using 34 (32) neurons. These findings improve significantly on our previous results and suggest an integrated approach to dynamically reading neural codes, measuring their properties, and quantifying the accuracy with which encoded information is extracted.