Modeling the conductance and DNA blockade of solid-state nanopores

We present measurements and theoretical modeling of the ionic conductance G of solid-state nanopores with 5-100 nm diameters, with and without DNA inserted into the pore. First, we show that it is essential to include access resistance to describe the conductance, in particular for larger pore diameters. We then present an exact solution for G of an hourglass-shaped pore, which agrees very well with our measurements without any adjustable parameters, and which is an improvement over the cylindrical approximation. Subsequently we discuss the conductance blockade ΔG due to the insertion of a DNA molecule into the pore, which we study experimentally as a function of pore diameter. We find that ΔG decreases with pore diameter, contrary to the predictions of earlier models that forecasted a constant ΔG. We compare three models for ΔG, all of which provide good agreement with our experimental data.     

Electrical properties of conductive Ge nanocrystal thin films fabricated by low temperature in situ growth

Thin films composed of Ge nanocrystals embedded in an amorphous SiO(2) matrix (Ge-NC TFs) were prepared using a low temperature in situ growth method. Unexpected high p-type conductivity was observed in the intrinsic Ge-NC TFs. Unintentional doping from shallow dopants was excluded as a candidate mechanism of hole generation. Instead, the p-type characteristic was attributed to surface state induced hole accumulation in NCs, and the hole conduction was found to be a thermally activated process involving charge hopping from one NC to its nearest neighbor. Theoretical analysis has shown that the density of surface states in Ge-NCs is sufficient to induce adequate holes for measured conductivity. The film conductivity can be improved significantly by post-growth rapid thermal annealing and this effect is explained by a simple thermodynamic model. The impact of impurities on the conduction properties was also studied. Neither compensation nor enhancement in conduction was observed in the Sb- and Ga-doped Ge-NC TFs, respectively. This could be attributed to the fact that these impurities are no longer shallow dopants in NCs and are much less likely to be effectively activated. Finally, the photovoltaic effect of heterojunction diodes employing such Ge-NC TFs was characterized in order to demonstrate its functionality in device implementation.     

MICROBIOLOGICAL PROFILE OF MURCHA STARTERS AND PHYSICO-CHEMICAL CHARACTERISTICS OF POKO, A RICE BASED TRADITIONAL FERMENTED FOOD PRODUCT OF NEPAL

Poko is a traditional rice based fermented food of Nepal prepared using murcha as the starter. The microbiological evaluation of eleven murcha starters showed that the lactic acid bacteria and yeast were dominant at 5×10⁵ to 1.0×10⁹ cfu g^-1 range while fungi were present at 2×10⁵ to 1.0×10⁷ cfu g^-1. Coliforms (1×10² to 1.4×10⁵ cfu g^-1), E. coli (1×10³ cfu g^-1), and. S. aureus (1×10² g^-1) were present in some of the murcha starters. Bacillus cereus was absent in all the starters. The microbial succession during poko fermentation suggested that it was primarily a mixed fermentation of yeasts, molds and lactic acid bacteria. The quality poko product obtained after two days and three days of fermentation at 30°C had a pH, acidity, reducing sugar, total sugar, and alcohol in the range of 3.2-3.0, 1.1-1.3 (% LA), 14.4-15.6 (%), 14.6-18.2 (%) and 1-1.6 (%) respectively. These critical ranges of biochemicals in the poko, due to the fermentation process imparted desirable organoleptic characteristics to the product. Saccharomyces cerevisiae, Candida versatilis, Lactobacillus spp, Pediococcus spp and Rhizopus spp were the dominant microorganisms identified from the poko fermentation. The results suggest that the quality of poko depends upon the microbial flora of the traditional murcha starters.     

Revealing Differential Expression of Phytohormones in Sorghum in Response to Aphid Attack Using the Metabolomics Approach

Sorghum (Sorghum bicolor) is an important multipurpose crop grown worldwide, but like many other crops, it is often threatened by insect pests. Sugarcane aphid (SCA, Melanaphis sacchari Zehntner), for example, is one of the most severe pests in sorghum, which causes plant damage and yield loss. The main objective of this study was to assess the effect of phytohormones on host plant resistance to aphid attack. Two sorghum genotypes, BTx623 (susceptible) and Tx2783 (resistant), were selected for a comparative analysis of differential expression of a group of phytohormones in response to aphid infestation. The quantification of phytohormones through LC-MS demonstrated higher levels of jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and auxins in the resistant genotype infested with SCA. The PCA plot supports the strong differential responses between resistant and susceptible genotypes, indicating a positive correlation between JA and ABA and a negative correlation between SA and auxins. Similarly, RT-PCR results of the phytohormones-related marker genes showed higher expression in the resistant genotype compared to the susceptible one. Furthermore, to corroborate the role of phytohormones in plant defense, the susceptible genotype was treated with SA, JA, and ABA. The exogenous application of SA and JA + ABA significantly reduced plant mortality, aphid number, and damage in the susceptible genotype, suggesting a strong correlation between phytohormones and plant survival. Our findings indicate that phytohormones play positive roles in plant defense against aphids and provide new insights into the molecular mechanisms operating in plants for self-protection. These findings could also stimulate further research into the mystery about the regulation of phytohormone production during plant interaction with aphids.     

Modification of EPDM with Alkylphenol Polysulfide for Use in Tire Sidewalls, 2 – Mechanistic and Morphological Characterizations

It has been shown that the mechanical properties of NR/BR/EPDM blends can be improved by using APPS as a modifier. In this paper, it is demonstrated that modification of EPDM with APPS takes place and saturation occurs when the APPS amount reaches 5 wt.‐%. It is shown that the rubber/carbon black affinity improves by using APPS‐grafted EPDM in the blend. TEM and STEM images clearly show that the NR/BR/APPS‐EPDM blend contains a significantly higher content of homogeneous rubber phase than the unmodified NR/BR/EPDM blend. The much better distribution of the carbon black in the APPS‐EPDM phase is considered the main reason for the improvement in properties of the blend.

     

HOST DEVELOPERS TO OBJECT TECHNICIANS Transition Strategies for OO Development

A company's decision to study and implement object technologies is just the beginning of a challenging and critical new role for software developers. Text-based, transaction processing-oriented programmers and designers can be retrained to take advantage of the opportunities object-oriented (OO) technology offers.     

Comparative studies on electronic transport due to the reduced dimensionality at the heterojunctions of GaAs/A1xGa(1−x)As and GaxIn(1−x)As/InP systems at low temperatures

Comparative studies on ac/dc mobility due to the reduced dimensionality of spatially confined low dimensional systems, at the heterojunctions of GaAs/A1_xGa_(1−x)As and Ga_xIn_(1−x)As/InP forming quasi-two dimensional (Q2D) and quasi-one dimensional (Q1D) systems have been made. The effect of various low temperature nonphonon scattering mechanisms such as ionized impurity, alloy disorder scattering and surface roughness scattering mechanisms; and phonon scattering mechanisms such as acoustic phonon via deformation potential and piezoelectric scattering mechanisms on the systems has been studied. It is found that the surface roughness scattering mechanism dominates in Q2D system whereas acoustic phonon scattering mechanism dominates in Q1D system due to which the nature and magnitude of the temperature dependent dc/ac mobility curves shows significant variation. Whereas, it is observed that the confinement does not change the nature of the frequency dependent real and imaginary parts of ac mobility curves. However, the mobility is found to be enhanced with effective mass and also due to the confinement, i.e. the mobility for Q1D system is higher than that for Q2D system.