杏(Prunus armeniaca)自交不亲和强度及其授粉受精相关特性

为了解杏自交不亲和性强度与授粉受精相关特性的关系,以自交亲和(Self-compatibility:SC)品种凯特(Prunus armeniaca L．cv．Katy)和自交不亲和(Self-incompatibility:SI)品种新世纪(P.armeniaca L.cv．Xinshiji)及凯特×新世纪杂种群体为试材,荧光显微镜观察自交亲和与自交不亲和杏花粉管生长动态。结果表明,授粉后初期,自交亲和性与自交不亲和性的杏花粉都能正常萌发、生长,但是在花粉管生长延伸到花柱1／2以后,自交亲和性的花粉管能顺利进入子房,而自交不亲和性的花粉管多数顶端膨大呈球形,停止向下生长,只有极个别能正常生长到达子房;杂种后代的可溶性蛋白含量和RNA酶比活力,与亲本相比无明显的趋中变异表现,而且在自交亲和与自交不亲和杏之间无显著性差异。     

阿魏菇工厂化栽培及常遇问题解决办法

利用阿魏菇（Pleurotus ferulae Lanzi）A0501菌株进行工厂化袋栽生产工艺试验。主要介绍了阿魏菇的生产工艺流程：母种的制备、原种的制备、茵袋的制备、接种发茵、后熟管理、搔茵与催蕾、修菇及育菇和出菇期管理，以及生产过程中出现茵袋污染和出菇期间发生病虫害的问题和解决办法。在控制污染的过程中，采用高压蒸汽灭菌，无菌过滤单元接种，对发茵棚进行严格的科学管理方法。针对病虫害的防治，采用不同的方法防治不同的病虫害，从而提高阿魏菇生产的经济效益。     

营口市设施果业发展规划

营口市设施果树起步较早,特别是设施桃、葡萄、李子、杏的发展处于全国领先地位。具有栽培品种多,降温早,冬季温室不用升温或升温简便等优势。     

鸡肠神经胆碱乙酰转换酶和多巴胺羟化酶细胞的免疫组织化学

应用SABC法免疫组织化学技术,探索鸡肠神经内胆碱乙酰转换酶（ChAc）和多巴胺羟化酶（DβH）神经元的形态及其分布。结果显示,肠神经内含有52%的ChAc神经元和36%的DβH神经元,2种神经元胞体或为多突起的多边形,或为一端有长突起的椭圆形,它们分布于神经内的神经纤维之间。DβH神经元在节间束也有个别分布。部分神经纤维呈ChAc或DβH阳性。这表明鸡肠神经系混合神经,既含副交感节后神经元,也含交感节后神经元。     

双环蘑菇的遗传学特性及品种选育研究进展

Advances in research on Agaricus bitorquis mating and breeding systems,and the breeding of new strains of this mushroom both in China and overseas,have been reviewed.Progress in our understanding of the genetic basis of homokaryotic fruiting,nuclear migration and mitochondrial inheritance,has been presented.The three main groups of A.bitorquis strains (temperate,bridging and tropical) categorized on the basis of mating behavior,and the distribution of homogenic and heterogenic incompatibility within the groups,have been discussed.Existing problems have been identified,and proposals made for future research.     

小菘菜花药培养获得再生植株

小菘菜,[Brassica campestns ssp.chinensis(L.)Makino]在日本普遍栽培近年我国引进其多个品种。作者拟通过单倍体育种途径选育小菘菜新品种来加快育种进程。以引自日本的小菘菜品种‘蕾用菜’(图版,A)为试材,种子经春化处理后穴盘育苗,盆栽。开花后选取健壮植株花序,选择小孢子发育处于单核靠边期至双核早期的花蕾(长2.5～3.5 mm),先用蒸馏水冲洗30 min,再用     

The latent gene expression of Epstein-Barr virus in nasopharyngeal carcinoma

AIM: To examine the latent membrane protein 1(LMP1)-DNA sequence in nasopharyngeal carcinoma(NPC) and detect mRNA expression of LMP1,EBNA1,EBNA2,and to explore the relationship between EBV infectious status,expression products and NPC carcinogenesis.METHODS: LMP1 DNA was detected in NPC by PCR.Direct sequence was applied to analyze the difference between NPC-LMP1-DNA and B95-8- LMP1-DNA.mRNA expressions of LMP1,EBNA1,EBNA2 in NPC were detected by nested RT-PCR.RESULTS: LMP1 DNA existed in all 47 NPC tissues.Several single nucleotide variations were found between NPC-LMP1-DNA and B95-8- LMP1-DNA.The notable variation was the lost of XhoⅠrestriction site in NPC.Direct sequence showed 30 bp deletion in NPC.The mRNA expressions of LMP1,EBNA1 and EBNA2 in NPC were 76.6%,80.0% and 74.5% respectively by nested RT-PCR.The expression of EBNA1 in NPC was promoted by Q promoter while the expression of EBNA1 in B95-8 was promoted by C promoter.CONCLUSION: The way of EBV involved in NPC is complex.Latent genes such as LMP1,EBNA1 and EBNA2 as well as early lytic gene BARF1 may all play certain roles in NPC carcinogenesis.     

鹅源H5N1亚型禽流感病毒感染SPF雏鸡免疫球蛋白含量的变化研究

以7日龄SPF雏鸡为试验动物,采用常规间接酶联免疫吸附试验(IELISA)法,对鹅源H5N1亚型禽流感病毒(AIV)感染SPF雏鸡后,其泪液、气管液、肠液、胆汁的IgA、IgG、IgM含量的动态变化进行了检测.结果发现,鹅源H5N1亚型中强毒AIV感染SPF雏鸡后,其泪液、气管液、肠液、胆汁的免疫球蛋白IgA、IgM含量均在1～3 d或1～4 d显著低于相应对照雏鸡(P＜0.05),而感染后第5天,上述被检指标开始升高,并于5～8 d或5～14 d,病毒感染雏鸡的IgA和IgM显著高于对照雏鸡(P＜0.05或P＜0.01).表明7日龄SPF雏鸡感染AIV后的早期,其消化道和呼吸道的体液免疫功能降低.