芹菜NAC转录因子基因AgNAC1的克隆及其对非生物胁迫的响应

通过RT-PCR方法,从芹菜‘六合黄心芹’和‘文图拉’中分别克隆获得编码NAC转录因子的基因AgNAC1。利用生物信息学方法分析其编码氨基酸序列组成、蛋白质理化性质、亲缘关系、空间结构等,采用荧光定量PCR技术检测基因在不同非生物胁迫下的表达水平。结果表明：‘六合黄心芹’和‘文图拉’AgNAC1开放阅读框长度均为957 bp,编码318个氨基酸,其蛋白质相对分子量分别为36.89和36.77 kD,理论等电点分别为5.94和5.78。AgNAC1蛋白与不同植物中NAC家族成员同源性比对和进化树分析表明,其与胡萝卜DcNAC属于同一个分支,进化距离最近。蛋白功能域预测显示,AgNAC1有多个α螺旋和β转角二级结构单元。荧光定量PCR结果表明,AgNAC1在芹菜叶中表达量最高,具有组织特异性,同时对高温、低温、干旱和盐胁迫均有响应。‘六合黄心芹’中AgNAC1的表达水平在高温处理24 h达到最高。‘文图拉’中AgNAC1的表达水平在高温、低温及盐处理后2 h和8 h高于对照,呈现先下降后上升的趋势,在干旱处理4 h时表达水平最高。     

盐碱胁迫对虞美人种子萌发的影响

以虞美人种子为试材,采用培养皿纸上萌发法,利用不同浓度梯度的盐溶液NaCl、碱溶液NaHCO_3以及NaCl与NaHCO_3(1∶1)混合溶液对虞美人种子进行处理,以期研究盐碱胁迫对虞美人种子萌发的影响。结果表明:盐碱溶液浓度的升高,抑制了种子萌发能力,种子的发芽率、相对发芽率、发芽势、苗鲜质量、胚根长、活力指数、发芽指数等指标均呈下降趋势;相对盐害率均呈上升趋势。虞美人种子的萌发对NaCl溶液的耐盐浓度为0.343%,极限浓度为0.935%;对NaHCO_3溶液的耐盐碱浓度为0.389%,极限浓度为0.950%;对NaCl与NaHCO_3(1∶1)混合溶液的耐盐碱浓度为0.425%,极限浓度为0.871%。     

Response of CREG1 promoter to serum starvation in human vascular cells

AIM: In order to explore the regulatory mechanisms of the human cellular repressor of E1A-stimulated genes (hCREG1) in vascular smooth muscle cells (VSMCs)-HITASY and in human umbilical vein endothelial cells (HUVECs), we clone and construct hCREG1 promoter vector to detect its expression in different vascular cells. METHODS: With the results of biological information, the fragments including -3 677 bp, -2 310 bp and -945 bp upstream sequences of hCREG1 were amplified respectively using human genomic DNA template by PCR. The products were inserted into pMD18-T vector, and then were subcloned into pEGFP-1 report vector to obtain the pEGFP-hCREG1-promoter vectors. The pEGFP-hCREG1-P3677, pEGFP-hCREG1-P2310 and pEGFP-hCREG1-P945 vectors were transfected into HITASY cells and HUVECs transiently and the expression of green fluorescent protein (GFP) was detected respectively by Western blotting and fluorescent microscope. RESULTS: It was confirmed that all three PCR fragments inserted into vectors were corrected by sequencing analysis. However, the expression of GFP was different significantly in both types of vascular cells. The expression of GFP in HUVECs was higher than that in HITASY cells. Meanwhile, the expression of GFP in HITASY cells with 0.5% FBS was increased obviously compared to that in HITASY cells with 10% FBS. CONCLUSION: The reporter vectors of hCREG1 promoter are constructed successfully in which the core promoter region might be located in -945 bp-0 bp of 5′ upstream sequences. This study will provide an experimental basis for exploring the regulation of hCREG1 expression.     

张家港地区不同防虫网覆盖方式下设施环境变化规律

通过使用智能型温湿度记录仪,以露地为对照,对夏季高温季节3种防虫网覆盖方式下(水平棚架覆盖、大棚覆盖、连栋大棚覆盖)设施内的空气温湿度进行连续记录,初步摸索出3种防虫网覆盖方式下设施内温湿度的变化规律。试验结果表明,高温晴天不同处理防虫网内的温度都高于露地,防虫网+连栋大棚模式设施内温湿度随着外界温度变化最大,温度最高时,防虫网+连栋大棚湿度最低,气温降低后湿度又升高得最快;雨天不同处理温度都差不多,防虫网+连栋大棚覆盖下湿度最低;阴天温度下午最高,各处理温度差别不大,防虫网+连栋大棚的湿度最低;雨转晴后防虫网+连栋大棚温度升高最快,湿度降低最快;所以防虫网+连栋大棚覆盖最适合夏季叶菜类栽培,高温低湿可有效防止烂菜,全封闭的生长和管理模式也可起到很好的防虫效果。     

嫁接对西瓜根际微生物种群数量及群落结构的影响

分别采用微生物培养的方法、16S rDNA PCR-RFLP方法和phl D基因PCR-RFLP方法研究了嫁接对西瓜根际微生物种群数量、细菌群落结构及拮抗菌（2, 4-DAPG产生菌）群落结构的影响。结果表明：① 嫁接西瓜根际细菌和真菌数量有所提高,而放线菌数量则有所减少。② 嫁接西瓜与自根西瓜具有1个相同的主要根际细菌基因型;不同砧木嫁接的西瓜与自根西瓜也分别具有不同基因型的细菌。③ 嫁接西瓜具有与自根西瓜不同的主要根际拮抗菌基因型。     

Hemin induces and sustains the phosphorylation of Erk1/2 in human umbilical vascular endothelial cells

AIM: To investigate the relationship between hemin and Erk1/2 activation in human umbilical vascular endothelial cells (HUVECs). METHODS: Cultured HUVECs were separately incubated with hemin or H₂O₂ for different times. Subsequently Erk1/2 phosphorylation and total Erk1/2 were determined by Western blotting assay. Flow cytometry was employed to determine the cell cycle distribution. RESULTS: Hemin at the concentration of 1-10 μmol/L induced the phosphorylation of Erk1/2 in HUVECs, and sustained the phosphorylation of Erk1/2 for three hours. The duration of phospho-Erk1/2 induced by hemin was much longer than that in H₂O₂ control (3 h vs 30 min). CONCLUSION: Hemin induces and sustains the phosphorylation of Erk1/2 in HUVECs, which indicates that the effect of hemin on the Erk1/2 activation may be one of pharmacological target of hemin.     

“新优选蕉柑”的选育研究

新优选蕉柑是从新1号蕉柑芽变单株中选育出来的蕉柑新品种。在潮汕地区经过多年对其后代进行多点区域性试验和品种比较试验,表明新优选蕉柑的枝梢较粗且长,叶片和花较大,果实明显增大,单果增大29 g左右;花量相对较少,着果率较高,丰产稳产性能更好,物候期和结果习性等与新1号蕉柑相近。新优选蕉柑是一个发展潜力较大的柑桔新品种,已经通过广东省农作物新品种审定。     

用雌性系配制的甜瓜新品种‘寿研1号’及其指纹图谱

 厚皮甜瓜‘寿研1号’是以回交转育的雌性系‘白9298gy’为母本, 以‘玉9635’自交系 为父本配制的一代杂交种。中熟, 果实发育期33～36 d。果实高圆形, 单瓜质量115～210 kg, 果皮光滑洁白, 果肉细腻, 白色略带浅绿, 糖度16.5%左右。易坐果, 耐低温, 抗病性强, 适于保护地栽培。用8对引物构建了其重复概率为1 /291 600的SSR分子标记的指纹图谱。     

生根粉在八仙花扦插繁殖中的应用研究简

在八仙花（Hydrangea macrophylla）扦插繁殖中,使用不同浓度的用生根粉配制的溶液,并进行不同施用方式及不同施用时间的处理。试验结果表明,合理使用生根粉,能够有效地提高八仙花扦插繁殖的生根率。     

紫花苜蓿叶片对硒胁迫的生理响应研究

以紫花苜蓿"大叶苜蓿""维多利亚""阿尔冈金"为试材,采用土培法,研究了硒胁迫对3种紫花苜蓿叶片酶促防御系统的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性及丙二醛(MDA)、可溶性蛋白质含量的影响。结果表明:在100μmol·L-1Se时,硒胁迫显著提高了3种紫花苜蓿叶片SOD、APX、POD、CAT活性和可溶性蛋白质含量。Se为900μmol·L-1时,SOD、APX活性和可溶性蛋白质、MDA含量显著增加,而CAT、POD活性显著降低。不同品种间抗氧化酶活性相比,"大叶苜蓿"抗氧化酶SOD、APX、POD、CAT活性显著高于其它2个苜蓿品种,且差异显著。综合评价表明,"大叶苜蓿"的抗氧化能力最强,其次为"阿尔冈金","维多利亚"苜蓿的抗氧化能力最差。