Interactions between phosphorus availability and microbes in a wheat–maize double cropping system: A reduced fertilization scheme

Mechanisms controlling phosphorus(P) availability and the roles of microorganisms in the efficient utilization of soil P in the wheat–maize double cropping system are poorly understood. In the present study, we conducted a pot experiment for four consecutive wheat–maize seasons(2016–2018) using calcareous soils with high(30.36 mg kg^–1) and low(9.78 mg kg^–1) initial Olsen-P content to evaluate the effects of conventional P fertilizer application to both wheat and maize(Pwm) ...     

The peroxisomal matrix shuttling receptor Pex5 plays a role in FB1 production and virulence in Fusarium verticillioides

The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling receptor FvPex5 is involved in the localization of PTS1,utilization of carbon sources and lipids,elimination ROS,cell wall stress,conidiation,fumonisin B₁(FB₁) production,and virulence in maize pathogen Fusarium verticillioides.Significantly,differential expression o...     

Expression of phenylalanine ammonia-lyase cDNA from rice in E. coli BL21DE3

AIM: To study the expression and its kinetics of rice phenylalanine ammonia-lyase gene encoding into E. coli as the basis of treatment for phenylketouria. METHODS: The phenylalanine ammonia-lyase-1-cDNA(rPAL-1-cDNA) from rice was recombined into E. coli high expression vector pET-28c and transformed into E. coli host strain BL21DE3. Engineering bacteria was then inducted by isopropyl-β-D-thiogalactoside (IPTG) for 1, 3, 5, 7 hours, in order to obtain high level expression. RESULTS: After induction, the expression level of fusion protein was 21.40%, 30.60%, 35.40%, 35.43% respectively. The fusion protein exhibited a band of 78.6 kD on SDS-PAGE analysis, but was not found in controls.The target protein was mainly existed in the form of inclusion body. CONCLUSION:Rice PAL gene expressing E. coli was established by gentic engineering technique.     

小麦蚜虫抗药性现状及综合治理策略

综述了麦蚜抗药性的形成和发展、抗药性现状、抗药性机理等,并提出了相应的综合治理策略。     

超级杂交稻国稻1号农艺性状研究

对超级杂交稻国稻1号和杂交稻汕优63的株型特征以及农艺性状进行了比较分析,结果表明:国稻1号的单产之所以显著高于汕优63的,主要因为前者分蘖多且分蘖快,单位面积有效穗数和每穗总粒数多,而且结实率高。     

根际效应下镉在土壤中的吸附与解吸

根际微区是联系植物与土壤之间的纽带,控制着重金属等污染物从土壤向植物的迁移.以黑土为研究对象,采用根际箱装置培养根际土壤,研究了不同强度玉米根际效应下,土壤对Cd的吸附与解吸.结果表明,相比于非根际土壤,根际土壤中的可溶性有机碳（DOC）含量显著增加,不同处理下根际土壤DOC含量与根系的干物质重量二者呈显著正相关（P＜0.001）;根际土壤pH显著提高.根际土壤对Cd的吸附量显著高于非根际土壤,且在较高的Cd浓度下这种差异表现的更明显,不同pH下土壤吸附试验表明,根际pH的升高是造成这种现象的主要原因.可以用Freundrich方程来描述Cd离子在根际效应下黑土中的吸附特征.根际土壤的解吸量显著低于非根际土壤,相关分析表明,平均的解吸百分数与根际土壤DOC含量呈显著负相关（P=0.011）,说明根系分泌的可溶性有机物质与Cd离子的络和作用,是导致根际土壤中Cd较难解吸的重要原因.     

谈图书馆讲座品牌的兴建——以山东省图书馆讲座为例

山东省图书馆于2006年3月开始与《齐鲁晚报》联合举办公益讲座,至今已近一年,其建立的品牌———大众讲坛的社会影响力逐渐增强。就山东省图书馆讲座的举办情况做一总结,以期能给新建立或拟建立自己品牌讲座的图书馆一点启示。     

由熊猫烧香病毒看图书馆网络安全

图书馆网络的安全涉及到计算机系统的硬件与软件的方方面面,针对新型病毒的产生,图书馆网络安全也应当适应新形势,采取必要的安全保护策略,合理构建网络安全系统。     

20份早熟欧美血缘玉米自交系利用潜力分析

以20份早熟欧美血缘自交系为供试材料,9份SS群自交系为母本、11份NSS群自交系为父本,采用不完全双列杂交设计,进行配合力分析评价。结果表明,自交系DNF342、DNLM18、东407、东409和东401产量一般配合力(GCA)优良,东304和东503株高、穗位高一般配合力优良,有利于降低杂交组合株高和穗位高。杂交组合东407×东601、东409×DNF342、东502×DN4206、东409×东305、东407×DN4206和东401×东601产量对照优势较强,可用于新品种试验。     

耐盐性不同的野大豆种子和幼苗对等渗水分和NaCl胁迫的响应

用等渗（-0．53MPa）PEG-6000、NaCl、Na^＋-盐和Cl^--盐溶液分别处理野大豆种群BB52（耐盐性较强）和N23232（耐盐性较弱）的种子和幼苗。结果表明：除PEG处理对两种群种子发芽无明显影响外,其余各等渗盐处理下其相对发芽率均显著下降,其中Na^＋-盐处理下降最明显;两种群所有处理幼苗的叶片相对电解质渗漏率和丙二醛（MDA）含量显著增加,叶绿素含量和叶绿素荧光参数值（PSⅡ最大光化学效率,Fv／Fm;光合电子传递速率,ETR）显著下降。这些处理对N23232的影响大于BB52,其中Na^＋-盐的影响最大,PEG影响最小。可见,NaCl胁迫对野大豆造成的伤害中,离子毒害较渗透胁迫重,其中又以Na^＋毒害大于Cl^-毒害。