Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.     

Radioligand binding assay of insulin receptor in rabbit kidney during ischemic and reperfusion

AIM: To observe the change of insulin receptor in rabbit kidney with acute ischemic-reperfusion injury. METHODS: 15 Japanese white rabbits were allocated randomly into control group, ischemic-reperfusion group(IR group). IR group received clamping for 1 h followed by 2 h or 48 h of reperfusion. At 2 h or 48 h after reperfusion, glucose and insulin in serum were determined. Insulin receptor in renal tissue was analyzed by radioligand binging assay(BAD). RESULTS: The level of serum glucose increased after 2 h reperfusion in 2 groups, but in IR group the value increased much more higher than those in control groups(P＜0.05). Plasma insulin of IR group was significantly higher than that in control after 2 h reperfusion(P＜0.05). Scatchard analysis of data resulted in curvilinear profiles, indicating that there are two classes of receptors with different affinity or the presence of a single class of receptors with a negative cooperative hormone-receptor interaction. Data analyzed for a two-site model showed that the values of Bmax₁(high affinity site), Bmax₂(low affinity site) and Kd₁, Kd₂ were significantly lower than that of control (P＜0.05) after 2 h perfusion. 48 h after IR there was no difference of Bmax₁, Bmax₂, and Kd₁ between 2 groups,but Kd₂ of IR group was higher than that of control (P＜0.05). CONCLUSIONS: The results indicate that the effect of intrinsic insulin decreases in the progress of the renal ischemic-reperfusion. The resulting high serum glucose may aggravate renal injury in the progress of ischemic-reperfusion.     

披碱草和野大麦杂种F1与BC1F1代的生物学及农艺特性研究

对披碱草和野大麦及其杂种F1与BC1F1代的生物学及农艺特性进行了分析比较。结果表明,亲本野大麦生长发育节律较快,较早地开花结实,果后营养期较长,生育期74d,生长天数达206d,具有很强的分蘖能力;披碱草生长发育节律较慢,生育期124d,生长天数193d;杂种F1的生长天数介于双亲之间,生长动态偏向亲本披碱草,分蘖能力介于双亲之间;BC1F1代生长发育节律较F1代提早,不同株系生长天数不同,生长动态偏向轮回亲本野大麦,分蘖能力有较大的变异。杂种F1代表现出较强的杂种优势,BC1F1代产草量较F1代有所降低,不同株系间存在明显的差异。总体上,BC1F1代的生产性能倾向于轮回亲本野大麦。     

pH及阳离子浓度对柞蚕蛹蛋白溶解性的影响

对不同pH及阳离子浓度下柞蚕蛹蛋白的溶解性进行了研究。结果表明,柞蚕蛹蛋白的等电点pI为4.2~4.5。测定不同的阳离子对其溶解度的影响顺序为Zn2+>Ca2+>Mg2+>Na+,随着阳离子浓度的增加,蛹蛋白的溶解性增加,当离子浓度超过1.0 mol/L时,其溶解性则降低。     

高速公路边坡防护体系的建植养护

在对广东省普惠高速公路边坡生物防治体系——护坡草皮的建植和养护过程中，就草种选择、建植方式和质量监控等问题进行了讨论并提出建议，旨在提高我国高速公路边坡防护体系草皮的建植和养护水平。     

春季休牧对草原和家畜生产力的影响

从4月上旬到6月下旬,在内蒙古锡林郭勒典型草原,进行春季休牧试验,对休牧和非休牧的草原进行生产力监测.结果表明:春季休牧可以有效地保护草原生态环境,经过两个月休牧后,草原植被状况明显改善;休牧期间采用低投入维持性饲养,可以显著降低饲养成本.并对休牧措施的生态效益、生产实践可行性和经济合理性进行综合讨论和评价.     

山羊生精上皮细胞分离研究

采用四种酶法分离二月龄关中奶山羊生精上皮细胞,A组:胶原酶Ⅳ;B组:胶原酶Ⅳ +透明质酸酶;C组:胶原酶Ⅳ+透明质酸酶+胰蛋白酶与EDTA;D组:胶原酶Ⅳ+透明质酸酶+胰蛋白酶与EDTA+DnaseI。结果二月龄羔羊曲细精管主要包含Sertoli细胞、精原细胞和管周排列的肌样细胞。每 200mg睾丸实质收获生精上皮细胞总数 (×105 )A,B,C,D组分别为 6. 91±0. 68, 6. 67±0. 80, 5. 94±0. 81, 5. 74±0. 82;细胞存活率 (% )分别为 76. 82±3. 80, 75. 96±1. 61, 94. 19±2. 89, 92. 32±1. 97;圆形细胞比率 (% )分别为 17. 54±1. 68, 16. 70±1. 46, 23. 64±1. 72, 22. 90±2. 38;细胞贴壁率(% )分别为 5. 93±0. 36, 5. 81±0. 54, 26. 94±1. 54, 25. 00±1. 74。C,D组不但组织块解离效果及细胞离散程度均较A,B好,且原代培养细胞团块数少。多重比较C,D组显著优于A,B组 (P<0.05);C组与D组差异不显著(P>0.05),但与A,B组比较细胞存活率、圆形细胞比率及贴壁率差异极显著(P<0.01)。结论认为C组是较简单有效的山羊生精上皮细胞分离酶组合。     

动物生物钟基因研究进展

昼夜节律生物钟是一种以近似24小时为周期的自主维持的振荡器,由输人通路、中央振荡器和输出通路三部分组成的。生物钟机制的研究已深入到分子水平。生物钟相关基因相继被分离鉴定,它们及其编码的蛋白质产物构成的自主调节的转录和翻译反馈环是生物钟运转的分子机制。本文介绍了果蝇和小鼠主要生物钟基因的发现以及其突变体对生物昼夜节律的影响,展望了揭示生物钟调节机制在遗传学上的重要意义。     

国内鹌鹑资源及其养殖现状和发展前景

介绍了关于野生鹌鹑遗传资源的种类、分布、迁徙规律以及被驯化后一些生活习性的改变和养殖现状,提出了我国在今后养殖鹌鹑产业中的机遇和挑战.